Experienced scientists working on protein engineering, expression, and purification, and cell line development services for decades. >90% success rate of stable cell line generation.
Stable cell lines developed with our proprietary protein expression system with multiple selectable markers is very productive in expressing target proteins.
Fast turnaround:
1 week for synthesizing a gene for expressing the target protein.
1 week for molecular construction of the gene.
5-6 weeks for generation of suspension CHO stable cell lines expressing the target protein.
5-6 weeks for additional services included in the premium package.BP004984: Human IL-31 Protein with the N-His Tag
Source: The recombinant Human IL-31 Protein, (amino acids Ser24-Thr164) fused with a His tag at the N-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human IL-31 protein with the N-His tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human IL-31 RA protein with the N-hFc tag with the EC50 of 1.53 ug/ml determined by ELISA (QC Test). The affinity constant of 1.5 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP003830: Human Latent TGF beta 3 Protein with the N-His Tag
Source: The recombinant Human Latent TGF beta 3 Protein, (amino acids Leu24-Ser412) fused with a His tag at the N-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human Latent TGF beta 3 protein with the N-His tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human TGF-beta RII protein with the N-mFc tag with the EC50 of 5.7 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP001128-C164: Recombinant Human Major prion protein (PRP)
Source: E. coli-derived.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 EU/ug).BP001560-PRO-461: Recombinant Human Cadherin-E (CDH1) Protein
Source: E. coli-derived.
Applications: ELISA, Inhibition Assays, WB.