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Generation of Stable Cell Lines Expressing Proteins

Stable Cell Line Development for Recombinant Protein Expression and Purification
Catalog No. Product Name Size List Price (US$) Quantity
BS067A Generation of Suspension CHO Clonal Stable Cell Lines Expressing Recombinant Proteins (Basic Service) 1 target gene 20000.00
BS067B Generation of Suspension CHO Clonal Stable Cell Lines Expressing Recombinant Proteins (Premium Service) 1 target gene 33000.00
BS067C Generation of Suspension CHO Pool Stable Cell Lines Expressing Recombinant Proteins 1 target gene 12000.00
Description

Stable cell lines expressing recombinant proteins are generated by experienced scientists working on protein engineering, expression, and purification, and cell line development services for decades. Stable cell lines developed with our proprietary protein expression system with multiple selectable markers is very productive in expressing target proteins.

Stable cell line development for protein production using CHO cells:

CHO suspension clonal stable cell lines are generated by electroporation using proprietary mammalian expression vector system with multiple selectable markers to express single gene of interest. Other mammalian stable cell lines, such as HEK 293 cells, can be used if requested.

Transfection into mammalian cells is followed by screening for cell lines permanently expressing specific genes through their incorporation into the cellular genome. The standard method for screening clones is ELISA. Additional fee will be charged for Western blot or cell-based assay of your interest. Our faster and more predictive screening techniques may significantly increase speed of stable cell line development while improving quality and uniformity.

Basic service of clonal stable cell line development for protein production (BS067A) includes:

a. Clone selection up to 24 clones;
b. Detailed characterization of the top clone during a 0.1 liter production run (viability, cell growth, and titer measurements);
c. Banking of 15 vials of the top clone (10 million cells per vial);
d. Backup vial storage for 6 months.

Deliverable:
a. 10 vials of the highest performing clone (10 million cells per vial);
b. All purified protein from 0.1 L production run;
c. Stable cell line development study report.

Timeline: 7-8 weeks.

Premium service of clonal stable cell line development for protein production (BS067B) includes basic service (BS067A) plus:

a. Subcloning the top 3 clones for a second round of selection including screening of up to 72 clones;
b. Top three clones selected from the second round and characterized for viability, cell growth, and titer measurements during a 0.1 liter production run;
c. Improved expression levels, stability, and purity compared to basic service;
d. Detailed characterization of the final banked clone during a 1 liter production run starting from thaw of a frozen vial (viability, cell growth, and titer measurements);
e. Backup vial storage for 1 year.

Deliverable:
a. 10 vials of the highest performing clone (15 million cells per vial);
b. All purified protein from all production runs;
c. Stable cell line development study report.

Timeline: 12-14 weeks.

Service of pool stable cell line development for protein production (BS067C) includes:

Deliverable:
a. 10 vials of the final pool (5 million cells per vial);
b. All purified protein from 0.1 liter production run;
c. Stable cell line development study report.

Fast turnaround: 6 weeks.

Syd Labs also provides the following stable cell line development services:

Stable Cell Line Generation Services for Antibody Production: development of stable cell lines for recombinant antibody expression.
Stable Cell Line Development Services for Biologics Production: generation of stable cell lines for manufacturing recombinant protein or antibody drugs.
Standard Stable Cell Line Development Services: non-viral generation of adhension or suspension stable cell lines with one or two gene insertion.
Lentiviral Based Stable Cell Line Generation Services: development of stable cell lines using lentivirus.

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