Description

The ChromaLink? Biotin Labeling Kit has everything you need and is faster and easier for labeling and measuring the amount of bound biotin than old traditional HABA:Avidin displacement measurement method. The Kit also has simple protocols on a flash drive and water soluble reagents, so it is safe, no organics needed. Highlights:

* Complete Kit, contains sufficient material to biotinylate five samples of 25 micrograms to 1 milligram
* Fast and easy method to biotinylate and determine the amount of labeling
* Label and determine the extent of biotin labeling with one reagent
* Simple calculations based on UV absorbance estimates total biotin incorporation (moles of biotin per mole of protein/peptide)
* Nondestructive assay does not destroy biotinylated sample Although widely used by researchers labeling antibodies and other molecules with biotin, the HABA:avidin method for estimating the extent of biotinylation has limitations.

Because there are not many other methods available for estimating the extent of biotinylation, researchers using the HABA:avidin method have learned to live with some disadvantages. ChromaLink Biotin accurately determines the degree of biotin incorporation without any of these disadvantages. This reagent, also available as a reagent only in both 5 ?1mg and 10mg formats, contains three important features that make it ideal for biotinylation:

1. A chromophore that absorbs strongly at 354 nm when coupled to protein; the chromophore, also covalently attached to the protein, is used to estimate the extent of labeling.
2. A long polyethylene glycol (PEG3) spacer, which reduces the tendency for aggregation of the labeled protein and minimizes steric hindrances related to the binding of biotin to biotin-binding proteins in affinity or detection applications.
3. An aromatic N-hydroxysuccinimide (NHS) ester reactive group, known to efficiently couple to amines, such as the e-amino group of lysine (K) or the a-terminus of the protein in aqueous systems.

Applications include:

* Biotinylate antibodies, protein or peptides
* Quantitate the extent of biotin labeling
* Optimize biotin-avidin complex.

Home

Services

Molecular Biology

Proteins & Peptides

Antibodies

ELISA Kits

Chemicals

Cell Biology

Contact

My Syd Labs

Email Notifications

Biotin Labeling Kit, Flashdrive

Note

Note

Order Offline