BP4200: Recombinant Dr. Nuclease (Benzonase Nuclease Alternative)

Source: E. coli-derived.
Specific activity: ≥1.1 x 10^6 U/mg. One unit of Dr. Nuclease is defined as the amount of enzyme required to produce a change in absorbance at 260 nm of 1.0 in the time of 30 minutes, under optimum conditions with excess substrate.
Purity: ≥95% by SDS-PAGE and ≥99% by SEC-HPLC. It does not contain any antimicrobial preservatives or protein stabilizers except glycerol (of synthetic origin).

BP4200-GMP: Recombinant Dr. Nuclease (Benzonase Nuclease Alternative), GMP Grade

Source: E. coli-derived.
Predicted molecular mass: ~28 kDa.
Specific activity: ≥1.1 x 10^6 U/mg. One unit of Dr. Nuclease is defined as the amount of enzyme required to produce a change in absorbance at 260 nm of 1.0 in the time of 30 minutes, under optimum conditions with excess substrate.
Purity: ≥95% by SDS-PAGE and ≥99% by SEC-HPLC. It does not contain any antimicrobial preservatives or protein stabilizers except glycerol (of synthetic origin).
Formulation: 0.2 μM filtered solution (250 units/ul) of 20 mM Tris-HCl, pH 8.0, 2 mM MgCl2, 2 mM NaCl, 50% Glycerol.
Endotoxin level: ≤0.01 EU/1000 units following 2020 ChP 1143 USP <85>.
Protease activity: No detectable protease activity using casein as substrate.
Residual host cell protein: ≤10.0 ppm by ELISA.
Mycoplasma: negative by qPCR method.
Bioburden: negative following 2020 ChP 1105 USP <61>.
Residual heavy metal: ≤10.0 ppm following 2020 ChP 0821 USP <32>.

BP003388: Mouse PD-L2/B7-DC Protein with the C-His Tag

Source: The recombinant mouse PD-L2/B7-DC protein, the PD-L2 ECD (amino acids Leu20-Trp221) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: The EC50 of the biotinylated recombinant human PD-1 protein with the hFc tag to the immobilized mouse PD-L2/B7-DC protein with the His tag at 5 ug/ml (100 ul/Well) was 0.32 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP004983: Biotinylated Human PD-L2/B7-DC Protein with the C-His-Avi Tag

Source: The recombinant human PD-L2/B7-DC protein, the PD-L2 ECD (amino acids Leu20-Thr220) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: The recombinant human PD-1 protein with the C-hFc Tag captured on CM5 Chip via Protein A can bind to the biotinylated recombinant human PD-L2 protein with the C-His-Avi tag with an affinity constant of 0.344 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005607: Human PD-L2/B7-DC Protein with the C-His-Avi Tag

Source: The recombinant human PD-L2/B7-DC protein, the PD-L2 ECD (amino acids Leu20-Thr220) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: The recombinant human PD-1 protein with the C-hFc Tag captured on CM5 Chip via Protein A can bind to the recombinant human PD-L2 protein with the C-His tag with an affinity constant of 0.3 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP003957: Cynomolgus PD-L2/B7-DC Protein with the C-His Tag

Source: The recombinant cynomolgus PD-L2/B7-DC protein, the PD-L2 ECD (amino acids Leu20-Thr220) fused with a His-tag at the C-terminus, was produced in HEK 293 cells.
Activity: The recombinant cynomolgus PD-1 protein with the C-hFc Tag captured on CM5 Chip via Protein A can bind to the recombinant cynomolgus PD-L2 protein with the C-His tag with an affinity constant of 0.17 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP004752: Biotinylated Mouse PD-L1/B7-H1 Protein with the C-His Tag

Source: The recombinant mouse PD-L1/B7-H1 protein, the PD-L1 ECD (amino acids Phe19-Arg238) fused with a His-tag at the C-terminus, was produced in HEK 293 cells.
Activity: The biotinylated recombinant mouse PD-L1 with the C-His Tag captured on CM5 Chip via the anti-His antibody can bind to the recombinant mouse PD-1 protein with the C-hFc Tag with an affinity constant of 0.31 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005535: Biotinylated Human PD-L1/B7-H1 Protein with the C-His Tag

Source: The recombinant human PD-L1/B7-H1 protein, the PD-L1 ECD (amino acids Phe19-Arg238) fused with a His-tag at the C-terminus, was produced in HEK 293 cells.
Activity: The EC50 of the biotinylated human PD-L1/B7-H1 protein with the His tag to the immobilized anti-human PD-L1 antibody with the hFc tag at 0.5 ug/ml (100 ul/Well) was 9.4 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005275: Mouse PD-L1/B7-H1 Protein with the C-His Tag

Source: The recombinant mouse PD-L1/B7-H1 protein, the PD-L1 ECD (amino acids Phe19-Thr238) fused with a His-tag at the C-terminus, was produced in HEK 293 cells.
Activity: The recombinant mouse PD-L1 with the C-His Tag captured on CM5 Chip via the anti-His antibody can bind to the recombinant mouse PD-1 protein with the C-hFc Tag with an affinity constant of 0.62 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005274: Mouse PD-L1/B7-H1 Protein with the C-hFc Tag

Source: The recombinant mouse PD-L1/B7-H1 protein, the PD-L1 ECD (amino acids Phe19-Arg237) fused with a hFc at the C-terminus, was produced in HEK 293 cells.
Activity: The recombinant human PD-1 with the C-His Tag captured on CM5 Chip via the anti-His antibody can bind to the recombinant mouse PD-L1 protein with the C-hFc Tag with an affinity constant of 92.49 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005345: Human PD-L1/B7-H1 Protein with the C-His Tag

Source: The recombinant human PD-L1/B7-H1 protein, the PD-L1 ECD (amino acids Phe19-Arg238) fused with a His-tag at the C-terminus, was produced in HEK 293 cells.
Activity: The EC50 of the anti-human PD-L1 antibody with the hFc tag to the immobilized human PD-L1/B7-H1 protein with the His tag at 0.5 ug/ml (100 ul/Well) was 6.4 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP003570: Human PD-L1/B7-H1 Protein with the C-His Tag

Source: The recombinant human PD-L1/B7-H1 protein, the PD-L1 ECD (amino acids Phe19-Ile226) fused with a His-tag at the C-terminus, was produced in HEK 293 cells.
Activity: The EC50 of the anti-human PD-L1 antibody with the hFc tag to the immobilized human PD-L1/B7-H1 protein with the His tag at 0.5 ug/ml (100 ul/Well) was 4.3 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP004986: Human PD-L1/B7-H1 Protein with the C-hFc Tag

Source: The recombinant human PD-L1/B7-H1 protein, the PD-L1 ECD (amino acids Phe19-Arg238) fused with a hFc at the C-terminus, was produced in HEK 293 cells.
Activity: The EC50 of the recombinant human PD-L1 protein with the hFc tag to the immobilized human PD-1/PDCD1  with the C-mFc tag at 1 ug/ml (100 ul/Well) was 0.17 ug/ml determined by ELISA. The recombinant human PD-L1 with the C-hFc Tag captured on CM5 Chip via Protein A can bind to the recombinant human PD-1 with the C-His Tag with an affinity constant of 2.20 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005315: Human PD-L1/B7-H1 Protein with the C-mFc Tag

Source: The recombinant human PD-L1/B7-H1 protein, the PD-L1 ECD (amino acids Phe19-Arg238) fused with a mFc at the C-terminus, was produced in HEK 293 cells.
Activity: The EC50 of the anti-human PD-L1 antibody with the hFc tag to the immobilized human PD-L1/B7-H1 protein with the C-mFc tag at 0.5 ug/ml (100 ul/Well) was 6.1 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP004753: Biotinylated Cynomolgus/Rhesus macaque PD-L1/B7-H1 Protein with the C-His Tag

Source: The recombinant cynomolgus/rhesus macaque PD-L1/B7-H1 protein, the PD-L1 ECD (amino acids Phe19-Arg238) fused with a His-tag at the C-terminus, was produced in HEK 293 cells.
Activity: The EC50 of the anti-cynomolgus PD-L1 antibody with the hFc tag to the immobilized biotinylated cynomolgus/rhesus macaque PD-L1/B7-H1 protein with the His tag at 1 ug/ml (100 ul/Well) was 4.0 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.