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Conditions of optimal recombinant human PD-L1/B7-H1 protein performance should be determined experimentally by the investigator. A promotion of 20% discount (coupon code PDL1protein24A20) is effective until June 30, 2024.
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BP005345: Human PD-L1/B7-H1 Protein with the C-His Tag
Source: The recombinant human PD-L1/B7-H1 protein, the PD-L1 ECD (amino acids Phe19-Arg238) fused with a His-tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant human PD-L1/B7-H1 protein with the C-His tag has a predicted MW of 26 kDa. Due to glycosylation, the protein migrates to 35-42 kDa based on Tris-Bis PAGE result.
Activity: The EC50 of the anti-human PD-L1 antibody with the hFc tag to the immobilized human PD-L1/B7-H1 protein with the His tag at 0.5 ug/ml (100 ul/Well) was 6.4 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant human PD-L1/B7-H1 protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
BP003570: Human PD-L1/B7-H1 Protein with the C-His Tag
Source: The recombinant human PD-L1/B7-H1 protein, the PD-L1 ECD (amino acids Phe19-Ile226) fused with a His-tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant human PD-L1/B7-H1 protein with the C-His tag has a predicted MW of 24.9 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
Activity: The EC50 of the anti-human PD-L1 antibody with the hFc tag to the immobilized human PD-L1/B7-H1 protein with the His tag at 0.5 ug/ml (100 ul/Well) was 4.3 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant human PD-L1/B7-H1 protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
BP004986: Human PD-L1/B7-H1 Protein with the C-hFc Tag
Source: The recombinant human PD-L1/B7-H1 protein, the PD-L1 ECD (amino acids Phe19-Arg238) fused with a hFc at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant human PD-L1/B7-H1 protein with the C-hFc tag has a predicted MW of 52 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result.
Activity: The EC50 of the recombinant human PD-L1 protein with the hFc tag to the immobilized human PD-1/PDCD1 with the C-mFc tag at 1 ug/ml (100 ul/Well) was 0.17 ug/ml determined by ELISA. The recombinant human PD-L1 with the C-hFc Tag captured on CM5 Chip via Protein A can bind to the recombinant human PD-1 with the C-His Tag with an affinity constant of 2.20 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant human PD-L1/B7-H1 protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
BP005315: Human PD-L1/B7-H1 Protein with the C-mFc Tag
Source: The recombinant human PD-L1/B7-H1 protein, the PD-L1 ECD (amino acids Phe19-Arg238) fused with a mFc at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant human PD-L1/B7-H1 protein with the C-mFc tag has a predicted MW of 50.9 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Tris-Bis PAGE result.
Activity: The EC50 of the anti-human PD-L1 antibody with the hFc tag to the immobilized human PD-L1/B7-H1 protein with the C-mFc tag at 0.5 ug/ml (100 ul/Well) was 6.1 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant human PD-L1/B7-H1 protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
Shipping: The recombinant human PD-L1/B7-H1 protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.
Recombinant PD1 and PDL1 Proteins:
Recombinant Human PD-1/PDCD1 Proteins
Biotinylated Recombinant Human PD-1/PDCD1 Proteins
Recombinant Mouse PD-1/PDCD1 Proteins
Biotinylated Recombinant Mouse PD-1/PDCD1 Proteins
Recombinant Cynomolgus PD-1/PDCD1 Proteins
Recombinant Canine PD-1/PDCD1 Proteins
PD1 and PDL1 Antibodies:
In vivo grade recombinant anti-mouse PD-1 monoclonal antibodies (Clone RMP1-14.1)
In vivo grade recombinant anti-mouse PD1 monoclonal antibodies (Clone 29F.1A12.1)
In vivo grade recombinant anti-mouse PD-L1 monoclonal antibodies (Clone 10F.9G2.1)
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