BP005190: Biotinylated Human GPRC5D Protein-VLP

Source: The recombinant Biotinylated Human GPRC5D Protein-VLP, (amino acids Met1-Val345) , was produced in HEK 293 cells.
Activity: Immobilized recombinant Human CD3E&CD3D, hFc tag at 1 ug/ml (100 ul/well) on the plate. Add serial dilutions of Anti-CD3×GPRC5D Bispecific Antibody, hFc tag in 10% Human Serum and then add various dilution ratio of Biotinylated Human GPRC5D VLP. Detection was performed using HRP-conjugated streptavidin with the EC50 of 75.5 ng/ml determined by ELISA. The affinity constant of 0.30 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005653: Biotinylated Human GM-CSF R alpha Protein with the C-His-Avi Tag

Source: The recombinant Biotinylated Human GM-CSF R alpha Protein, (amino acids Glu23-Gly320) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Anti-GM-CSF R alpha Antibody protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human GM-CSF R alpha protein with the C-His tag with the EC50 of 11.6 ng/ml determined by ELISA (QC Test). The affinity constant of 1.68 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP004413: Biotinylated Human GITR/TNFRSF18 Protein with the C-His-Avi Tag

Source: The recombinant Biotinylated Human GITR/TNFRSF18 Protein, (amino acids Gln26-Glu161) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human GITR Ligand Trimer protein with the C-His-Avi tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human GITR protein with the C-His tag with the EC50 of 77.9 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005023: Biotinylated Human GITR Ligand/TNFSF18 Trimer Protein with the N-His-Flag Tag

Source: The recombinant Biotinylated Human GITR Ligand/TNFSF18 Trimer Protein, (amino acids Gln50-Ser177) fused with a His-Flag tag at the N-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human GITR protein with the N-His-Flag tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human GITR Ligand Trimer protein with the N-His tag with the EC50 of 11.4 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005022: Biotinylated Human GFRAL/GFR alpha-like Protein with the C-His-Avi Tag

Source: The recombinant Biotinylated Human GFRAL/GFR alpha-like Protein, (amino acids Ser19-Glu351) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human GDF15 protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human GFRAL protein with the C-His tag with the EC50 of 89.1 ng/ml determined by ELISA (QC Test).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP004144: Biotinylated Human GDF15 Protein with the N-His-Avi Tag

Source: The recombinant Biotinylated Human GDF15 Protein, (amino acids Ala197-Ile308) fused with a His-Avi tag at the N-terminus, was produced in E.coli cells.
Activity: Immobilized recombinant Human GFRAL protein with the N-His-Avi tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Biotinylated Human GDF15 protein with the N-His tag with the EC50 of 24 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005010: Biotinylated Human GDF15 Protein with the N-hFc Tag

Source: The recombinant Biotinylated Human GDF15 Protein, (amino acids Ala197-Ile308) fused with a hFc tag at the N-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human GFRAL protein with the N-hFc tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Biotinylated Human GDF15 protein with the N-hFc tag with the EC50 of 7.0 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP003195: Biotinylated Human GDF15 (H202D) Protein with the N-hFc Tag

Source: The recombinant Biotinylated Human GDF15 (H202D) Protein, (amino acids Ala197-Ile308 (H202D)) fused with a hFc tag at the N-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human GFRAL protein with the N-hFc tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human GDF15 (H202D) protein with the N-hFc tag with the EC50 of 4.4 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005119: Biotinylated Human GARP&Latent TGF beta 1 Complex Protein with the C-His-Avi Tag

Source: The recombinant Biotinylated Human GARP&Latent TGF beta 1 Complex Protein, (amino acids His20-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1)) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Anti-GARP&Latent TGF beta 1 Antibody protein with the C-His-Avi tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human GARP&Latent TGF Beta 1 Complex protein with the C-His tag with the EC50 of 44.0 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005635: Biotinylated Human FOLR1 Protein with the C-His-Avi Tag

Source: The recombinant Biotinylated Human FOLR1 Protein, (amino acids Arg25-Met233) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Anti-FOLR1 Antibody protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Biotinylated Human FOLR1 protein with the C-His tag with the EC50 of 13.9 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP003358: Biotinylated Human FLT3/Flk-2 Protein with the C-His-Avi Tag

Source: The recombinant Biotinylated Human FLT3/Flk-2 Protein, (amino acids Asn27-Asn541) fused with a His-Avi tag at the C-terminus, was produced in CHO cells.
Activity: Immobilized recombinant Human FLT3 Ligand protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human FLT3 protein with the C-His tag with the EC50 of 0.26 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.