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Conditions of optimal recombinant Biotinylated Human GARP&Latent TGF beta 1 Complex Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
BP005119: Biotinylated Human GARP&Latent TGF beta 1 Complex Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human GARP&Latent TGF beta 1 Complex Protein, (amino acids His20-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1)) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Biotinylated Human GARP&Latent TGF beta 1 Complex protein with the His-Avi tag at the C-terminus has a predicted MW of 70.3 kDa (GARP)&41.4 kDa (Latent TGF beta 1). Due to glycosylation, the protein migrates to 73-78 kDa (GARP)&13 kDa&42-45 kDa (Latent TGF beta 1) based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Anti-GARP&Latent TGF beta 1 Antibody protein with the C-His-Avi tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human GARP&Latent TGF Beta 1 Complex protein with the C-His tag with the EC50 of 44.0 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Biotinylated Human GARP&Latent TGF beta 1 Complex Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
Shipping: The recombinant Biotinylated Human GARP&Latent TGF beta 1 Complex Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.
Syd Labs provides the following GARP&Latent TGF beta 1 Complex Protein :
Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex Protein
Biotinylated Human GARP&Latent TGF beta 1 Complex Protein
Biotinylated Mouse GARP&Latent TGF beta 1 Complex Protein
Biotinylated Rat GARP&Latent TGF Beta 1 Complex Protein
Cynomolgus GARP&Latent TGF beta 1 Complex Protein
Human GARP&Latent TGF Beta 1 Complex Protein
Mouse GARP&Latent TGF beta 1 Complex Protein
Rat GARP&Latent TGF Beta 1 Complex Protein
Syd Labs provides the following GARP&Latent TGF beta 2 Complex Protein :
Human GARP&Latent TGF Beta 2 Complex Protein
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