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Bst DNA Polymerase Large Fragment

Enzymes for LAMP, Loop-Mediated Isothermal Amplification

Catalog No. Product Name Size List Price (US$) Quantity
MB121 Bst 2G DNA Polymerase, Large Fragment 1600 units 49.00
MB121 Bst 2G DNA Polymerase, Large Fragment 8000 units 196.00
MB122 Bst DNA Polymerase, Large Fragment 1600 units 49.00
MB122 Bst DNA Polymerase, Large Fragment 8000 units 196.00
Description

MB121: Bst 2G DNA Polymerase, Large Fragment

The Bst 2G DNA Polymerase, Large Fragment, an engineered or mutated version of a part of Bacillus stearothermophilus DNA polymerase, has the 5’→3’ DNA polymerase activity and the strong strand displacement activity, but lacks the 5’→3’ exonuclease activity. The Bst 2G DNA Polymerase, Large Fragment can be used for loop-mediated isothermal amplification (LAMP), Rolling-circle amplification (RCA), DNA sequencing through high GC regions, and rapid sequencing from nanogram amounts of DNA template. The engineered Bst 2G DNA Polymerase, Large Fragment is optimized for LAMP with better amplification speed and yield, salt tolerance and thermostability than its wild type version.

Unit definition: One unit of the Bst DNA Polymerase, Large Fragment is defined as the amount of enzyme that will incorporate 25 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.

Concentration: 8 units/ul.

The amplification or synthesis reagent concentrations, reaction conditions, and program parameters should be determined experimentally by the investigator.

Features of the Bst 2G DNA Polymerase, Large Fragment:
* Rapid amplification of diverse templates.
* Higher amplification yield.
* Increased tolerance of inhibitors including salts.
* Improved thermostability of the enzyme.
* The cheapest Bst 2.0 DNA Polymerase, Large Fragment in the market.

Applications of the Bst 2G DNA Polymerase, Large Fragment:
* Loop-mediated isothermal DNA amplification (LAMP)
* Rolling-circle amplification (RCA)
* DNA sequencing through problemtic secondary regions such as GC rich part
* Rapid sequencing from nanogram amounts of DNA template.

The cheap Bst 2G DNA Polymerase, Large Fragment is also available in the cheap RT-LAMP kits.

Please contact us if you need protocol, datasheet, or SDS of our Bst DNA Polymerases, Large Fragment.

MB122: Bst DNA Polymerase, Large Fragment

The Bst DNA Polymerase, Large Fragment, the native or wild type version of a part of Bacillus stearothermophilus DNA polymerase, has the 5’→3’ DNA polymerase activity and the strong strand displacement activity, but lacks the 5’→3’ exonuclease activity. The Bst DNA Polymerase, Large Fragment can be used for loop-mediated isothermal DNA amplification (LAMP), Rolling-circle amplification (RCA), DNA sequencing through high GC regions, and rapid sequencing from nanogram amounts of DNA template. 

Unit definition: One unit of the Bst DNA Polymerase, Large Fragment is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.

Concentration: 8 units/ul.

The amplification or synthesis reagent concentrations, reaction conditions, and program parameters should be determined experimentally by the investigator.

Features of the Bst DNA Polymerase, Large Fragment:
* Warm-start reaction system: The reaction process is completed by simply mixing all components at 60 - 65 ℃ for 30 - 60 minutes.
* Multiple options of reading results: The amplification products can be checked with gel electrophoresis, colorimetric methods, and fluorescence intensity methods.
* Potent amplification ability: The upgraded polymerase features strong 5´→3´ DNA polymerase activity and strong strand displacement activity.
* Perfect for assembling RT-LAMP kits: Compatible with various reverse transcriptases from Syd Labs and other commercial suppliers.
* The cheapest Bst 1.0 DNA Polymerase, Large Fragment in the market.

Applications of the Bst DNA Polymerase, Large Fragment:
* Loop-mediated isothermal DNA amplification (LAMP)
* Rolling-circle amplification (RCA)
* DNA sequencing through problemtic secondary regions such as GC rich part
* Rapid sequencing from nanogram amounts of DNA template.

PCR enzymes:
* Taq DNA polymerase.
* High yield Taq DNA polymerase.
* Hot-start high-fidelity DNA polymerase.
* Hot start Taq DNA polymerase with chemical or antibody inhibitor, used for SYBR green or Taqman probe-based real time PCR (qPCR or quantitative PCR).

2x PCR master mixes:
* 2x PCR PreMix with dye (green, red, blue).
* 2x rapid PCR Master Mix with a green dye.
* 2x long PCR Master Mix with a blue dye.
* 2x high yield PCR master mix with a blue dye.
* 2x hot-start high-fidelity PCR master mix with a blue dye.
* Blood direct PCR kit
* Cell and tissue direct PCR kit
* RT-PCR master mixes
* 2x qPCR master mixes
* RT-qPCR master mixes

PCR components and related products:
* dNTP mix, 10 mM each.
* dNTP mix, 25 mM each.
* Proteinase K powder.
* Proteinase K solution.
* Reverse Transcriptases.
* First strand cDNA synthesis kits.
* Murine RNase inhibitor.
* PCR enhancer.

Questions and Answers:

Question: Do you provide free sample of your hot start Taq DNA polymerase?
Answer: Sorry that we do not provide free sample. You can place an order and we will hold the invoice until you tell us that you like our product. You do not need to pay us if you do not like the product. It is an alternative to free sample. 

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