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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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The cheapest probe-based multiplex one-step qRT-PCR kit (5x qRT-PCR master mixes with UDG) in the market. The low cost probe-based one-step qRT-PCR kits (5x qRT-PCR master mixes with UDG) and SYBR Green dye-based one-step qRT-PCR kit (2x qRT-PCR master mix) are also available.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
The probe-based multiplex one-step qRT-PCR kit is ready-to-use 5x concentrated solution for multiplex, quantitative, reverse transcription-qPCR. Except DNA template and primers, the 5x probe-based multiplex one-step qRT-PCR master mix contains all other components for RT and qPCR, including dNTP/dUTP mix, MgCl2, reaction buffer, 5G Reverse Transcriptases, murine RNase inhibitor, heat-labile UDG, and antibody-based hot start Taq DNA polymerase at optimal concentrations for consistent and efficient PCR amplification. For a 20 ul reaction, simply add 4 ul of 5x probe-based multiplex one-step qRT-PCR master mix to specific primers, DNA template, 50x ROX reference dye 1 and 2, and water.
The probe-based multiplex one-step qRT-PCR kit is specially designed for single-plex or multiplex qPCR detection using RNA (e.g. SARS-COV-2 virus RNA) as template. It has extremely high stability, which can be stored at 37℃ for 14 days and its performance remains unchanged, and remains liquid form at a storage temperature of - 20℃ to avoid the thawing process of reagents. The reaction can be started by directly adding the template, primers and target probes required for detection. Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination. The probe-based multiplex one-step qRT-PCR kit contains a dUTP/UDG system that can prevent the contamination of PCR product. Heat-labile UDG can quickly eliminate the contamination of dU-containing products at room temperature. It can be inactivated at 55℃ without affecting the efficiency and sensitivity of qRT-PCR. Combining the superior performance of 5G Reverse Transcriptase and antibody-based hot-start Taq DNA Polymerase, with an optimized buffering system, the detection sensitivity of the probe-based multiplex one-step qRT-PCR kit can reach 0.1 pg of total RNA or less than 10 copies of RNA templates and is suitable for high-specificity detection systems based on fluorescence labelled probes (e.g. TaqMan).
Optimal template and primer concentrations, and qPCR program parameters should be determined experimentally by the investigator.
Features of the Probe-Based Multiplex One-Step qRT-PCR Kit:
* Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination.
* More sensitive amplification that is suitable for DNA and RNA templates.
* More stable amplification curve with low concentration template.
* Better storage conditions.
* It supports rapid procedures and is suitable for rapid reactions.
Components of the Probe-Based Multiplex One-Step qRT-PCR Kit:
Probe-based multiplex one-step qRT-PCR master mix (5X): Buffer optimized for reverse transcription-qPCR; Mixtures of dNTP/dUTP mix, MgCl2, reaction buffer, 5G Reverse Transcriptases, murine RNase inhibitor, heat-labile UDG, and antibody-based hot start Taq DNA polymerase.
ROX reference dye 1 (50X).
ROX reference dye 2 (50X).
RNase-free H2O.
Storage/Stability: Stable for 1 year at -70°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.
PCR enzymes:
* Taq DNA polymerase.
* High yield Taq DNA polymerase.
* Bst DNA polymerase large fragment.
* Hot-start high-fidelity DNA polymerase.
* Hot start Taq DNA polymerase with chemical or antibody inhibitor, used for SYBR green or Taqman probe-based real time PCR (qPCR or quantitative PCR).
2x PCR master mixes:
* 2x PCR preMix with dye (green, red, blue).
* 2x rapid PCR master mix with a green dye.
* 2x long PCR master mix with a blue dye.
* 2x high yield PCR master mix with a blue dye.
* 2x hot-start high-fidelity PCR master mix with a blue dye.
* Blood direct PCR kit
* Cell and tissue direct PCR kit
* RT supermix for qPCR.
* RT-PCR Kit.
* Dye-based one-step qRT-PCR kits.
* Probe-based one-step qRT-PCR kits.
* Probe-based multiplex one-step qRT-PCR kits.
* SYBR Green qPCR master mixes.
* Probe qPCR master mixes.
PCR components and related products:
* dNTP mix, 10 mM each and dNTP mix, 25 mM each.
* Proteinase K powder and Proteinase K solution.
* Reverse transcriptases and First strand cDNA synthesis kits.
* Murine RNase inhibitor.
* Uracil DNA glycosylases.
* PCR enhancer.
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