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PCR Enhancer

GC-Rich PCR Enhancer

Catalog No. Product Name Size List Price (US$) Quantity
MB040-EE-1 PCR Enhancer 1 ml 17.00

MB040: GC Rich PCR Enhancer

The GC Rich PCR enhancer can improve the efficiency of PCR amplification of GC rich DNA templates, increase the specificity of PCR products, and reduce non-specific and undesirable PCR products. During conventional PCR analysis, it is difficult to amplify DNA fragments with high GC content due to their stable secondary structures. The problem may be solved without modifying the Taq enzyme. The GC rich PCR enhancer is a mixed additives composed of multiple components, which can effectively reduce the melting temperature of high GC templates as well as other templates with complex secondary structures. The GC rich PCR enhancer is compatible with almost all DNA polymerase amplification systems.

The PCR reagent concentrations, reaction conditions, and PCR program parameters should be determined experimentally by the investigator. A typical reaction:

10× Taq buffer (with 15 mM MgCl2 ):  5 µl
dNTP mix (10 mM each):                    1 µl
PCR Enhancer:                                 10 µl
Template DNA:                               100 ng
Primer F (10 µM):                               2 µl
Primer R (10 µM):                               2 µl
Taq DNA polymerase (5 U/µl):         0.4 µl
ddH2O:                                         to 50 µl

Features of the GC rich PCR enhancer:
* High amplification efficiency and stability.
* Reproducible results.
* The cheapest PCR enhancer in the market.

Applications of the GC rich PCR enhancer:
* Routine PCR using genomic, viral and plasmid templates.
* DNA and colony screening.
* Genotyping.
* DNA mutagenesis.

The cheap GC rich PCR enhancer is also available in the cheap 2X PCR PreMix, with dye (catalog No. MB067-EQ2).

Please contact us if you need protocol, datasheet, or SDS of the GC rich PCR enhancer.

PCR enzymes:
* Taq DNA polymerase.
* High yield Taq DNA polymerase.
* Bst DNA polymerase large fragment.
* Hot-start high-fidelity DNA polymerase.
* Hot start Taq DNA polymerase with chemical or antibody inhibitor, used for SYBR green or Taqman probe-based real time PCR (qPCR or quantitative PCR).

2x PCR master mixes:
* 2x PCR preMix with dye (green, red, blue).
* 2x rapid PCR master mix with a green dye.
* 2x long PCR master mix with a blue dye.
* 2x high yield PCR master mix with a blue dye.
* 2x hot-start high-fidelity PCR master mix with a blue dye.
* Blood direct PCR kit
* Cell and tissue direct PCR kit
* RT supermix for qPCR.
* RT-PCR Kit.
* Dye-based one-step qRT-PCR kits.
* Probe-based one-step qRT-PCR kits.
* Probe-based multiplex one-step qRT-PCR kits.
* SYBR Green qPCR master mixes.
* Probe qPCR master mixes.

PCR components and related products:
* dNTP mix, 10 mM each and dNTP mix, 25 mM each.
* Proteinase K powder and Proteinase K solution.
* Reverse transcriptases and First strand cDNA synthesis kits.
* Murine RNase inhibitor.
* Uracil DNA glycosylases.
* PCR enhancer.

Questions and Answers:

Question: Do you provide free sample of your Taq DNA polymerase?
Answer: Sorry that we do not provide free sample. You can place an order and we will hold the invoice until you tell us that you like our product. You do not need to pay us if you do not like the product. It is an alternative to free sample.  

Question: How to make homemade Taq polymerase?
Answer: It is always heard that scientists can put a Taq polymerase gene into an expression vector and produce large quantity of Taq enzyme in their labs with low cost. It is not so easy as one imagines. You need tools for bacterial protein expression and purification in addition to DNA subcloning. Genomic and plasmid DNA has to be removed during protein purification to produce DNA free Taq polymerase. There is residual DNA in most, if not all, of homemade Taq polymerase. The buffer also has to be optimized for activity and stability of the PCR enzyme. With our best deal for bulk order and reliable formulation of Taq DNA polymerase assay kit, it is really unnecessary for scientists to produce even large quantity of homemade Taq enzyme.

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