Syd Labs has extensive experience with making DNA constructs for Fc fusion protein production, expressing and purifying a number of Fc fusion proteins from microgram to kilogram scales in mammalian cells, such as CHO and HEK 293 cells. With improved DNA construction system and fully optimized secretion signal and linker sequences, Syd Labs can efficiently complete a project from DNA construction and transient production of Fc fusion proteins in CHO and HEK 293 cells, to Fc fusion protein purification. N-term or C-term of Fc fusions of peptides and proteins in secreted form and multiple Fc backbone are available upon request.
BS050B:
Synthesis of the gene encoding the Fc fusion protein;
Molecular construction of the recombinant Fc fusion protein;
0.04 Liter transient production of the recombinant Fc fusion protein in CHO or HEK293 cells;
Protein A purification.BS046B:
Gene synthesis of the variable regions of the recombinant IgG antibodies;
Molecular construction of the recombinant IgG antibodies;
0.04 Liter transient production of the recombinant IgG antibodies in CHO cells;
Protein A purification.IA021: NMab Protein A Resin
* Cost competitive, high binging capacity alkaline-stable protein A resins.
* High dynamic binding capacity: ≥ 55 mg/mL (human IgG, 4 minute residence time).
* Clean in Place: 0.1-0.5 M NaOH.
IA019: Alkaline-Stabilized Recombinant Protein A
Our alkaline-stabilized recombinant protein A represents the most advanced mutant Protein A ligand, and is used in all of our alkaline-stabilized recombinant protein A resins with Clean in Place: 0.1-0.5 M NaOH.Pre-Packed NMab Protein A Columns
A variety of pre-packed protein A affinity columns are available with NMab protein A resin, NMab Pro protein A resin, and UniMab 50HC Protein A resin in a range of flexible column diameters and bed heights. The pre-packed NMab protein A columns provide solid all-round performance, low Protein A leakage, good binding capacity and useful effective working range - all at an excellent value.
EK106-9000-1: Protein A ELISA kit for detection of natural and recombinant Protein A
* Sensitivity of 0.1 ug/ml (0.8 ppm)
* Accuracy/Recovery: 80-120%
* Precision: Inter-assay 10%, Intra-assay 15%
* Specificity for all variants of natural and recombinant Protein A
* 2 hour assay completion timeEK106-9333-1: Protein A ELISA kit for detection of MabSelect SuReTM ligand
* Sensitivity of 0.1 ug/ml
* Accuracy/Recovery: 80-120%
* Precision: Inter-assay 10%, Intra-assay 15%
* Specificity for MabSelect SuReTM ligand
* 2 hour assay completion timeBP4091: In Vivo Grade Recombinant Human IgG1 Fc protein (hIgG1 Fc)
The recombinant human IgG1 Fc protein was produced in mammalian cells and purified by protein A.
Purity: >95% by SDS-PAGE under reducing conditions
Endotoxin: Less than 1 EU/mg of protein as determined by LAL method.Recombinant human IgG1 Fc protein mutants available including hIgG1 Fc C103S; hIgG1 Fc D265A; hIgG1 Fc R214K; hIgG1 Fc R214K/N297A; hIgG1 Fc L234A/L235A; hIgG1 Fc L234F/L235E/P331S; hIgG1 Fc L235A/G237A. Recombinant human IgG1 isotype control antibody and mutants are available. Biotinylated hIgG1 Fc, and hIgG1 Fc with Avi-, His-, and Flag-tags are also available.
BP4092: In vivo Grade Recombinant Human Fc protein (IgG2)
The recombinant human IgG2 Fc protein was produced in HEK 293 cells and purified by protein A.
Purity: >95% by SDS-PAGE under reducing conditions
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.BP4093: In Vivo Grade Recombinant Human IgG4 Fc protein
The recombinant human IgG4 Fc protein was produced in HEK 293 cells and purified by protein A.
Purity: >95% by SDS-PAGE under reducing conditions
Endotoxin: Less than 1 EU/mg of protein as determined by LAL method.