- IA000020-1042: Protein G
Recombinant Protein-G used in Protein G beads is a genetically engineered form of Protein-G. Non-essential regions have been removed while leaving the IgG binding sites intact.
Protein G has been extensively used for the isolation of IgG from several species of mammals. Although binding of Protein G to IgG's is not equivalent for all IgG subclasses, Protein-G possesses properties that have made it a popular choice for antibodies isolation. Protein G binds immunoglobulins at the Fc region of leaving the Fab region free to bind the antigen. Hence, Protein G is extremely useful for isolating of immune complexes. BP002404-PRO-402: Recombinant Protein G
Source: E. coli-derived.
Specificity: 1. Binds with greater affinity to most mammalian immunoglobulins than Protein A, including human IgG3 and rat IgG2a. 2. Does not bind to human IgM, IgD and IgA.
Applications: Protein G binds to the constant region of many species of immunoglobulin G. It can be used to detect, quantify and purify IgG antibodies and antibody/antigen complexes.
Purity: > 95% as determined by SDS-PAGE and RP-HPLC.
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