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Recombinant anti-NP isotype control antibodies and Fc silenced mutants from clone B1-8 good for in vitro and in vivo studies. Low or no specific binding to human, rat, and mouse samples tested. Low prices for bulk order.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Background
The anti-NP B1-8 antibody (IgG2c, kappa) in nature is suitable for use as an isotype-matched control antibody to estimate the degree of non-specific binding by an antigen-specific antibody. The recombinant B1-8 IgG2c kappa isotype control and mutants (natural and engineered mIgG2c isotype controls) are useful for in vitro and in vivo studies from ELISA, Western blot (WB), to cell-based assay to animal model study. The mouse IgG2c antibody is produced in certain inbred strains such as C57BL/6, C57BL/10, SJL, and NOD, which does express the mouse IgG2a antibody, and vice versa. If one uses any of the mouse strains for animal research, it is better to use the IgG2c antibodies rather than the IgG2a antibodies. There are more and more IgG antibody formats with Fc engineered. The format with the Fc silenced, Fc silent, or Fcs with silenced effector function is the most popular. Thus, the wild type mouse IgG2c isotype control and the Fc silenced mouse IgG2c isotype control mutants should be used in such an application.
The in vivo grade recombinant B1-8 mouse IgG2c isotype control antibody (mIgG2c isotype control) was produced in mammalian cells.
Immunogen: hapten-protein conjugated NP12-chicken gammaglobulin (CG); Hapten: 4-hydroxy-3-nitrophenyl acetyl (NP).
Clone: B1-8.
Isotype: mouse IgG2c, kappa.
Applications: an isotype-matched negative control for anti-NP mouse IgG2c kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Shipping: The in vivo grade recombinant anti-NP mouse IgG2c kappa isotype control antibody (clone of B1-8) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.
In Vivo Grade Recombinant anti-NP Isotype Control Antibodies (Clone B1-8):
In vivo Grade Recombinant B1-8 Mouse IgG2c Isotype Control Antibody Mutants
In vivo Grade Recombinant B1-8 Mouse IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant B1-8 Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant B1-8 Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant B1-8 Mouse IgG3 Isotype Control Antibody and Mutants
In vivo Grade Recombinant B1-8 Mouse Fab Fragment
In vivo Grade Recombinant B1-8 Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant B1-8 Human IgG2 Isotype Control Antibody and Mutants
In vivo Grade Recombinant B1-8 Human IgG3 Isotype Control Antibody and Mutants
In vivo Grade Recombinant B1-8 Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant B1-8 Human Fab Fragment
In vivo Grade Recombinant B1-8 Human IgE Isotype Control Antibody
In vivo Grade Recombinant B1-8 Human IgM Isotype Control Antibody
In vivo Grade Recombinant B1-8 Human IgA1 Isotype Control Antibody
In vivo Grade Recombinant B1-8 Rat IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant B1-8 Rat IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant B1-8 Rat IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant B1-8 Rat IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant B1-8 Rat Fab Fragment
In vivo Grade Recombinant B1-8 Hamster IgG1 Isotype Control Antibody
In vivo Grade Recombinant B1-8 Hamster IgG2 Isotype Control Antibody
In vivo Grade Recombinant B1-8 Rabbit IgG Isotype Control Antibody
In vivo Grade Recombinant B1-8 Ferret IgG1 Isotype Control Antibody
In vivo Grade Recombinant B1-8 Ferret IgM Isotype Control Antibody
In vivo Grade Recombinant B1-8 Ferret IgA Isotype Control Antibody
In vivo Grade Recombinant B1-8 Canine IgG1 Isotype Control Antibody
In vivo Grade Recombinant B1-8 Canine IgG2 Isotype Control Antibody
In vivo Grade Recombinant B1-8 Canine IgG3 Isotype Control Antibody
In vivo Grade Recombinant B1-8 Canine IgG4 Isotype Control Antibody
In vivo Grade Recombinant B1-8 Feline IgG1 Isotype Control Antibody
Other Recombinant IgG Reference Antibodies:
In vivo Grade Recombinant Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Human IgG2 Isotype Control Antibody
In vivo Grade Recombinant Human IgG3 Isotype Control Antibody
In vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG3 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody
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