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B1-8 Isotype Control Antibodies

Recombinant B1-8 Isotype Control, B1-8 Negative Control Antibody

Catalog No.	Product Name	Size	List Price (US$)
PA007151.m1	In vivo Grade Recombinant B1-8 Mouse IgG1 Isotype Control Antibody	5 mg	595.00
PA007151.m2a	In vivo Grade Recombinant B1-8 Mouse IgG2a Isotype Control Antibody	5 mg	595.00
PA007151.m2b	In vivo Grade Recombinant B1-8 Mouse IgG2b Isotype Control Antibody	5 mg	595.00
PA007151.m2c	In vivo Grade Recombinant B1-8 Mouse IgG2c Isotype Control Antibody	5 mg	595.00
PA007151.m3	In vivo Grade Recombinant B1-8 Mouse IgG3 Isotype Control Antibody	5 mg	595.00
PA007151.mf	In vivo Grade Recombinant B1-8 Mouse Fab Fragment	1 mg	900.00
PA007151.h1	In vivo Grade Recombinant B1-8 Human IgG1 Isotype Control Antibody	5 mg	595.00
PA007151.h2	In vivo Grade Recombinant B1-8 Human IgG2 Isotype Control Antibody	5 mg	595.00
PA007151.h3	In vivo Grade Recombinant B1-8 Human IgG3 Isotype Control Antibody	5 mg	595.00
PA007151.h4	In vivo Grade Recombinant B1-8 Human IgG4-S228P Isotype Control Antibody	5 mg	595.00
PA007151.hf	In vivo Grade Recombinant B1-8 Human Fab Fragment	1 mg	900.00
PA007151.he	In vivo Grade Recombinant B1-8 Human IgE Isotype Control Antibody	1 mg	650.00
PA007151.hm	In vivo Grade Recombinant B1-8 Human IgM Isotype Control Antibody	1 mg	650.00
PA007151.ha	In vivo Grade Recombinant B1-8 Human IgA1 Isotype Control Antibody	1 mg	650.00
PA007151.rt1	In vivo Grade Recombinant B1-8 Rat IgG1 Isotype Control Antibody	5 mg	595.00
PA007151.rt2a	In vivo Grade Recombinant B1-8 Rat IgG2a Isotype Control Antibody	5 mg	595.00
PA007151.rt2b	In vivo Grade Recombinant B1-8 Rat IgG2b Isotype Control Antibody	5 mg	595.00
PA007151.rt2c	In vivo Grade Recombinant B1-8 Rat IgG2c Isotype Control Antibody	5 mg	595.00
PA007151.rf	In vivo Grade Recombinant B1-8 Rat Fab Fragment	1 mg	900.00
PA007151.hs1	In vivo Grade Recombinant B1-8 Hamster IgG1 Isotype Control Antibody	5 mg	595.00
PA007151.hs2	In vivo Grade Recombinant B1-8 Hamster IgG2 Isotype Control Antibody	5 mg	595.00
PA007151.r	In vivo Grade Recombinant B1-8 Rabbit IgG Isotype Control Antibody	5 mg	595.00
PA007151.f1	In vivo Grade Recombinant B1-8 Ferret IgG1 Isotype Control Antibody	1 mg	650.00
PA007151.fm	In vivo Grade Recombinant B1-8 Ferret IgM Isotype Control Antibody	1 mg	650.00
PA007151.fa	In vivo Grade Recombinant B1-8 Ferret IgA Isotype Control Antibody	1 mg	650.00
PA007151.c1	In vivo Grade Recombinant B1-8 Canine IgG1 Isotype Control Antibody	1 mg	650.00
PA007151.c2	In vivo Grade Recombinant B1-8 Canine IgG2 Isotype Control Antibody	1 mg	650.00
PA007151.c3	In vivo Grade Recombinant B1-8 Canine IgG3 Isotype Control Antibody	1 mg	650.00
PA007151.c4	In vivo Grade Recombinant B1-8 Canine IgG4 Isotype Control Antibody	1 mg	650.00
PA007151.fg1	In vivo Grade Recombinant B1-8 Feline IgG1 Isotype Control Antibody	1 mg	650.00

Note

Recombinant anti-NP isotype control antibodies and Fc silenced mutants from clone B1-8 good for in vitro and in vivo studies. Low or no specific binding to human, rat, and mouse samples tested. Low prices for bulk order.
Note

Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
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Description

Background

The anti-NP B1-8 antibody (IgG2c, kappa) in nature is suitable for use as an isotype-matched control antibody to estimate the degree of non-specific binding by an antigen-specific antibody. The recombinant B1-8 IgG2c kappa isotype control and mutants (natural and engineered mIgG2c isotype controls) are useful for in vitro and in vivo studies from ELISA, Western blot (WB), to cell-based assay to animal model study. The mouse IgG2c antibody is produced in certain inbred strains such as C57BL/6, C57BL/10, SJL, and NOD, which does express the mouse IgG2a antibody, and vice versa. If one uses any of the mouse strains for animal research, it is better to use the IgG2c antibodies rather than the IgG2a antibodies. There are more and more IgG antibody formats with Fc engineered. The format with the Fc silenced, Fc silent, or Fcs with silenced effector function is the most popular. Thus, the wild type mouse IgG2c isotype control and the Fc silenced mouse IgG2c isotype control mutants should be used in such an application.

PA007151.m2c: In Vivo Grade Recombinant B1-8 Mouse IgG2c Kappa Isotype Control Antibody (mIgG2c Isotype Control)

The in vivo grade recombinant B1-8 mouse IgG2c isotype control antibody (mIgG2c isotype control) was produced in mammalian cells.
Immunogen: hapten-protein conjugated NP12-chicken gammaglobulin (CG); Hapten: 4-hydroxy-3-nitrophenyl acetyl (NP).
Clone: B1-8.
Isotype: mouse IgG2c, kappa.
Applications: an isotype-matched negative control for anti-NP mouse IgG2c kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The in vivo grade recombinant anti-NP mouse IgG2c kappa isotype control antibody (clone of B1-8) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.

References of B1-8 Isotype Control Antibodies:

Mouse IgG1, κ Isotype Ctrl (clone B1-8).
Not specified. (2025). BioLegend Product Datasheet. doi: Not specified. PMID: Not specified
The clone B1-8 recognizes the DNP hapten and serves as an isotype control for mouse IgG1, κ antibodies in various applications, including flow cytometry, immunoprecipitation, Western blotting, and immunohistochemical staining. The suggested concentration for flow cytometric staining is ≤ 0.5 µg per 10^6 cells in 100 µL volume. C57BL/6 mouse splenocytes were stained with Mouse IgG1, κ Isotype Ctrl (clone B1-8) FITC. The antibody has been quality-tested by immunofluorescent staining with flow cytometric analysis.
Tags: activity of B1-8 isotype control; B1-8 isotype control antibody

Mouse IgG1, κ Isotype Ctrl (clone B1-8) - Purified.
Not specified. (2025). BioLegend Product Datasheet. doi: Not specified. PMID: Not specified
The clone B1-8, specific for the DNP hapten, is used as an isotype control for mouse IgG1, κ antibodies. This purified antibody is suitable for flow cytometry, immunofluorescence microscopy, immunohistochemistry (paraffin or frozen), and Western blotting. Each lot is tested by immunofluorescent staining with flow cytometric analysis. C57BL/6 mouse splenocytes were stained with Mouse IgG1, κ Isotype Ctrl (clone B1-8) Purified followed by anti-mouse IgG FITC. The recommended concentration for flow cytometric staining is ≤ 0.5 µg per 10^6 cells in 100 µL volume.
Tags: B1-8 isotype control antibody for animal model; B1-8 isotype control antibody in vivo

Anti-DNP Antibody (clone B1-8) - Mouse IgG1, κ Isotype Control.
Not specified. (2025). BioLegend Product Datasheet. doi: Not specified. PMID: Not specified
The B1-8 isotype control antibody (clone B1-8) is specific for DNP (2,4-dinitrophenol) hapten and serves as a negative control for mouse IgG1, κ antibodies. It is quality-tested for flow cytometry with no detectable binding to mouse antigens. The recommended concentration for flow cytometric staining is ≤ 0.5 µg per million cells in 100 µL volume. BALB/c splenocytes were stained with B1-8 isotype control (clone B1-8) followed by anti-mouse IgG1 FITC. The antibody should be stored at 2–8°C and protected from prolonged light exposure.
Tags: B1-8 isotype control for cancer research; B1-8 isotype control for flow cytometry

Mouse IgG1 kappa Isotype Control (14-4714-82).
Not specified. (2025). Thermo Fisher Scientific Product Datasheet. doi: Not specified. PMID: Not specified
The monoclonal mouse IgG1 kappa isotype control (clone B1-8) is used as an isotype control immunoglobulin. It has been reported for use in surface and intracellular flow cytometric analysis, immunohistochemistry, immunocytochemistry, immunoprecipitation, and immunoblotting (WB). The antibody has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse spleen cells, and it should be used at the same concentration as the experimental antibody. Purity is greater than 90%, as determined by SDS-PAGE.
Tags: B1-8 isotype control for mouse tumor model; B1-8 isotype control dose

Purified Mouse IgG1 kappa Isotype Control (14-4714-81).
Not specified. (2025). Thermo Fisher Scientific Product Datasheet. doi: Not specified. PMID: Not specified
The monoclonal mouse IgG1 kappa isotype control (clone B1-8) serves as an isotype control immunoglobulin. It is reported for use in surface and intracellular flow cytometric analysis, immunohistochemistry, immunocytochemistry, immunoprecipitation, and immunoblotting (WB). The antibody has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse spleen cells, and it should be used at the same concentration as the experimental antibody. Mouse IgG1 is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.
Tags: in vivo study of B1-8 isotype control antibody; B1-8 isotype control in vivo antibody

For more references about B1-8 isotype control antibodies please contact our scientific support team with message@sydlabs.com.

In Vivo Grade Recombinant anti-NP Isotype Control Antibodies (Clone B1-8):
In vivo Grade Recombinant B1-8 Mouse IgG2c Isotype Control Antibody Mutants
In vivo Grade Recombinant B1-8 Mouse IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant B1-8 Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant B1-8 Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant B1-8 Mouse IgG3 Isotype Control Antibody and Mutants
In vivo Grade Recombinant B1-8 Mouse Fab Fragment
In vivo Grade Recombinant B1-8 Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant B1-8 Human IgG2 Isotype Control Antibody and Mutants
In vivo Grade Recombinant B1-8 Human IgG3 Isotype Control Antibody and Mutants
In vivo Grade Recombinant B1-8 Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant B1-8 Human Fab Fragment
In vivo Grade Recombinant B1-8 Human IgE Isotype Control Antibody
In vivo Grade Recombinant B1-8 Human IgM Isotype Control Antibody
In vivo Grade Recombinant B1-8 Human IgA1 Isotype Control Antibody
In vivo Grade Recombinant B1-8 Rat IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant B1-8 Rat IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant B1-8 Rat IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant B1-8 Rat IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant B1-8 Rat Fab Fragment
In vivo Grade Recombinant B1-8 Hamster IgG1 Isotype Control Antibody
In vivo Grade Recombinant B1-8 Hamster IgG2 Isotype Control Antibody
In vivo Grade Recombinant B1-8 Rabbit IgG Isotype Control Antibody
In vivo Grade Recombinant B1-8 Ferret IgG1 Isotype Control Antibody
In vivo Grade Recombinant B1-8 Ferret IgM Isotype Control Antibody
In vivo Grade Recombinant B1-8 Ferret IgA Isotype Control Antibody
In vivo Grade Recombinant B1-8 Canine IgG1 Isotype Control Antibody
In vivo Grade Recombinant B1-8 Canine IgG2 Isotype Control Antibody
In vivo Grade Recombinant B1-8 Canine IgG3 Isotype Control Antibody
In vivo Grade Recombinant B1-8 Canine IgG4 Isotype Control Antibody
In vivo Grade Recombinant B1-8 Feline IgG1 Isotype Control Antibody

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B1-8 Isotype Control Antibodies

Recombinant B1-8 Isotype Control, B1-8 Negative Control Antibody

Note

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PA007151.m2c: In Vivo Grade Recombinant B1-8 Mouse IgG2c Kappa Isotype Control Antibody (mIgG2c Isotype Control)

References of B1-8 Isotype Control Antibodies: