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HyHEL-10 Isotype Control Antibodies

Anti-HEL Isotype Control, Recombinant HyHEL-10 Isotype Control, HyHEL-10 Negative Control Antibody

Catalog No. Product Name Size List Price (US$) Quantity
PA007154.m1 In vivo Grade Recombinant HyHEL-10 Mouse IgG1 Isotype Control Antibody 1 mg 160.00
PA007154.m2a In vivo Grade Recombinant HyHEL-10 Mouse IgG2a Isotype Control Antibody 1 mg 160.00
PA007154.m2b In vivo Grade Recombinant HyHEL-10 Mouse IgG2b Isotype Control Antibody 1 mg 160.00
PA007154.m2c In vivo Grade Recombinant HyHEL-10 Mouse IgG2c Isotype Control Antibody 1 mg 160.00
PA007154.m3 In vivo Grade Recombinant HyHEL-10 Mouse IgG3 Isotype Control Antibody 1 mg 160.00
PA007154.mf In vivo Grade Recombinant HyHEL-10 Mouse Fab Fragment 1 mg 500.00
PA007154.h1 In vivo Grade Recombinant HyHEL-10 Human IgG1 Isotype Control Antibody 1 mg 160.00
PA007154.h2 In vivo Grade Recombinant HyHEL-10 Human IgG2 Isotype Control Antibody 1 mg 160.00
PA007154.h3 In vivo Grade Recombinant HyHEL-10 Human IgG3 Isotype Control Antibody 1 mg 160.00
PA007154.h4 In vivo Grade Recombinant HyHEL-10 Human IgG4-S228P Isotype Control Antibody 1 mg 160.00
PA007154.hf In vivo Grade Recombinant HyHEL-10 Human Fab Fragment 1 mg 500.00
PA007154.he In vivo Grade Recombinant HyHEL-10 Human IgE Isotype Control Antibody 1 mg 295.00
PA007154.hm In vivo Grade Recombinant HyHEL-10 Human IgM Isotype Control Antibody 1 mg 295.00
PA007154.ha In vivo Grade Recombinant HyHEL-10 Human IgA1 Isotype Control Antibody 1 mg 295.00
PA007154.rt1 In vivo Grade Recombinant HyHEL-10 Rat IgG1 Isotype Control Antibody 1 mg 160.00
PA007154.rt2a In vivo Grade Recombinant HyHEL-10 Rat IgG2a Isotype Control Antibody 1 mg 160.00
PA007154.rt2b In vivo Grade Recombinant HyHEL-10 Rat IgG2b Isotype Control Antibody 1 mg 160.00
PA007154.rt2c In vivo Grade Recombinant HyHEL-10 Rat IgG2c Isotype Control Antibody 1 mg 160.00
PA007154.rf In vivo Grade Recombinant HyHEL-10 Rat Fab Fragment 1 mg 500.00
PA007154.hs1 In vivo Grade Recombinant HyHEL-10 Hamster IgG1 Isotype Control Antibody 1 mg 160.00
PA007154.hs2 In vivo Grade Recombinant HyHEL-10 Hamster IgG2 Isotype Control Antibody 1 mg 160.00
PA007154.r In vivo Grade Recombinant HyHEL-10 Rabbit IgG Isotype Control Antibody 1 mg 160.00
PA007154.f1 In vivo Grade Recombinant HyHEL-10 Ferret IgG1 Isotype Control Antibody 1 mg 295.00
PA007154.fm In vivo Grade Recombinant HyHEL-10 Ferret IgM Isotype Control Antibody 1 mg 295.00
PA007154.fa In vivo Grade Recombinant HyHEL-10 Ferret IgA Isotype Control Antibody 1 mg 295.00
PA007154.c1 In vivo Grade Recombinant HyHEL-10 Canine IgG1 Isotype Control Antibody 1 mg 295.00
PA007154.c2 In vivo Grade Recombinant HyHEL-10 Canine IgG2 Isotype Control Antibody 1 mg 295.00
PA007154.c3 In vivo Grade Recombinant HyHEL-10 Canine IgG3 Isotype Control Antibody 1 mg 295.00
PA007154.c4 In vivo Grade Recombinant HyHEL-10 Canine IgG4 Isotype Control Antibody 1 mg 295.00
PA007154.fg1 In vivo Grade Recombinant HyHEL-10 Feline IgG1 Isotype Control Antibody 1 mg 295.00
Description

Background

The anti-HEL monoclonal antibody (hen egg-white lysozyme) in nature is suitable for use as an isotype-matched control antibody to estimate the degree of non-specific binding by an antigen-specific antibody. The recombinant mouse IgG1 kappa isotype control (clone number: HyHEL-10) and mutants (natural and engineered mIgG1 isotype controls) are useful for in vitro and in vivo studies from ELISA, Western blot (WB), to cell-based assay to animal model study. The isotypes of mouse IgG antibody reagents used in scientific research and clinical diagnostics are mainly mouse IgG1 kappa. Mouse IgG1 antibodies and engineered mutants are also used in therapeutic drug discovery. Mouse IgG1 antibodies are more like human IgG4 antibodies than human IgG1 antibodies, which behave more like mouse IgG2a or IgG2c antibodies. Many antibody drugs especially those targetting immune checkpoint proteins use the human IgG4 constant regions.

As an ideal recombinant mouse IgG1 isotype control,  the recombinant HyHEL-10 mIgG1 kappa isotype control has low or no specific binding to any mouse sample, and has completely mouse variable regions and the mouse IgG1 kappa constant regions. The anti-HEL mouse IgG1 kappa isotype control and mutants can be used in ELISA, Western blot (WB), the cell-based assay or animal model as the antibody negative control.

Please contact us to ask for a quote for the engineered HyHEL-10 isotype control mutants, isotype control and mutants with Avi-, His-, and Flag-tags, and biotinylated isotype control and mutants. A variety of conjugates (such as dyes and fluorophores) with the anti-HEL isotype control and mutants are available.

PA007154.m1: In Vivo Grade Recombinant HyHEL-10 Mouse IgG1 Kappa Isotype Control Antibody (mIgG1 Isotype Control)

The in vivo grade recombinant HyHEL-10 mouse IgG1 isotype control antibody (mIgG1 isotype control) was produced in mammalian cells.
Immunogen: hen egg-white lysozyme (HEL).
Clone: HyHEL-10.
Isotype: mouse IgG1, kappa.
Applications: an isotype-matched negative control for anti-HEL mouse IgG1 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The in vivo grade recombinant anti-HEL mouse IgG1 kappa isotype control antibody (clone of HyHEL-10) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.

In Vivo Grade Recombinant anti-HEL Isotype Control Antibodies (clone HyHEL-10):
In vivo Grade Recombinant HyHEL-10 Mouse IgG1 Isotype Control Antibody Mutants
In vivo Grade Recombinant HyHEL-10 Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant HyHEL-10 Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant HyHEL-10 Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant HyHEL-10 Mouse IgG3 Isotype Control Antibody and Mutants
In vivo Grade Recombinant HyHEL-10 Mouse Fab Fragment
In vivo Grade Recombinant HyHEL-10 Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant HyHEL-10 Human IgG2 Isotype Control Antibody and Mutants
In vivo Grade Recombinant HyHEL-10 Human IgG3 Isotype Control Antibody and Mutants
In vivo Grade Recombinant HyHEL-10 Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant HyHEL-10 Human Fab Fragment
In vivo Grade Recombinant HyHEL-10 Human IgE Isotype Control Antibody
In vivo Grade Recombinant HyHEL-10 Human IgM Isotype Control Antibody
In vivo Grade Recombinant HyHEL-10 Human IgA1 Isotype Control Antibody
In vivo Grade Recombinant HyHEL-10 Rat IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant HyHEL-10 Rat IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant HyHEL-10 Rat IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant HyHEL-10 Rat IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant HyHEL-10 Rat Fab Fragment
In vivo Grade Recombinant HyHEL-10 Hamster IgG1 Isotype Control Antibody
In vivo Grade Recombinant HyHEL-10 Hamster IgG2 Isotype Control Antibody
In vivo Grade Recombinant HyHEL-10 Rabbit IgG Isotype Control Antibody
In vivo Grade Recombinant HyHEL-10 Ferret IgG1 Isotype Control Antibody
In vivo Grade Recombinant HyHEL-10 Ferret IgM Isotype Control Antibody
In vivo Grade Recombinant HyHEL-10 Ferret IgA Isotype Control Antibody
In vivo Grade Recombinant HyHEL-10 Canine IgG1 Isotype Control Antibody
In vivo Grade Recombinant HyHEL-10 Canine IgG2 Isotype Control Antibody
In vivo Grade Recombinant HyHEL-10 Canine IgG3 Isotype Control Antibody
In vivo Grade Recombinant HyHEL-10 Canine IgG4 Isotype Control Antibody
In vivo Grade Recombinant HyHEL-10 Feline IgG1 Isotype Control Antibody

Other Recombinant IgG Reference Antibodies:
In vivo Grade Recombinant Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Human IgG2 Isotype Control Antibody
In vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody

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