C1.18.4 Isotype Control Antibodies

Background

The C1.18.4 antibody (IgG2a, kappa) in nature is suitable for use as an isotype-matched control antibody to estimate the degree of non-specific binding by an antigen-specific antibody. The recombinant C1.18.4 IgG2a kappa isotype control and mutants (natural and engineered mIgG2a isotype controls) are useful for in vitro and in vivo studies from ELISA, Western blot (WB), to cell-based assay to animal model study. Mouse IgG2a antibodies are similar to human IgG1 antibodies in many aspects, thus researchers like to use the isotype of mouse IgG2a in mouse studies. Most of the therapeutic antibody drugs carry the natural human IgG1 constant regions. More and more engineered mutants in the human IgG1 Fc fragment have been tested during therapeutic antibody drug development. The corresponding mutations have been introduced to mouse IgG2a to study enhancement and abrogation effects of Fc binding. We generated recombinant C1.18.4 mIgG2a isotype control mutants, the same mutations as those in the therapeutic antibodies and candidates because such mouse IgG2a isotype control mutants are needed when clients test their engineered antibody candidates. For example, P329G substitution combined with L234A L235A (mIgG2a LALAPG, Fc silenced) was shown to decrease effector functions by disrupting the Fc/Fcγ receptor interface.

As an ideal recombinant mouse IgG2a isotype control,  the recombinant C1.18.4 mIgG2a kappa isotype control has low or no specific binding to any mouse sample, and completely mouse variable regions and the mouse IgG2a kappa constant regions. The mouse IgG2a kappa isotype control and mutants can be used in ELISA, Western blot (WB), the cell-based assay or animal model as the antibody negative control.

Please contact us to ask for a quote for the engineered C1.18.4 mouse IgG2a isotype control mutants, mIgG2a isotype control and mutants with Avi-, His-, and DYKDDDDK-tags, and biotinylated mIgG2a isotype control and mutants. A variety of conjugates (such as dyes and fluorophores) with the C1.18.4 mouse IgG2a isotype control and mutants are available.

PA007152.m2a: In Vivo Grade Recombinant C1.18.4 Mouse IgG2a Kappa Isotype Control Antibody (mIgG2a Isotype Control)

The in vivo grade recombinant C1.18.4 mouse IgG2a isotype control antibody (mIgG2a isotype control) was produced in mammalian cells.
Immunogen: N/A.
Clone: C1.18.4.
Isotype: mouse IgG2a, kappa.
Applications: an isotype-matched negative control for mouse IgG2a kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The in vivo grade recombinant C1.18.4 mouse IgG2a kappa isotype control antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.

References of C1.18.4 Isotype Control Antibodies:

Rat IgG2b, ? Isotype Ctrl (C1.18.4).
Not specified. (2025). BioLegend Product Datasheet. doi: Not specified. PMID: Not specified
The clone C1.18.4 recognizes the DNP hapten and serves as an isotype control for rat IgG2b, ? antibodies in various applications, including flow cytometry, immunoprecipitation, Western blotting, and immunohistochemical staining. The suggested concentration for flow cytometric staining is ? 0.25 ?g per 10^6 cells in 100 ?L volume. C57BL/6 mouse splenocytes were stained with Rat IgG2b, ? Isotype Ctrl (clone C1.18.4) FITC. The antibody has been quality-tested by immunofluorescent staining with flow cytometric analysis.
Tags: activity of C1.18.4 isotype control antibody; C1.18.4 isotype control

Rat IgG2b, ? Isotype Ctrl (C1.18.4).
Not specified. (2025). BioLegend Product Datasheet. doi: Not specified. PMID: Not specified
The clone C1.18.4, specific for the DNP hapten, is used as an isotype control for rat IgG2b, ? antibodies. This antibody is suitable for flow cytometry, immunofluorescence microscopy, immunohistochemistry (paraffin or frozen), and Western blotting applications. Each lot is tested by immunofluorescent staining with flow cytometric analysis. C57BL/6 mouse splenocytes were stained with Rat IgG2b, ? Isotype Ctrl (clone C1.18.4) PE. The recommended concentration for flow cytometric staining is ? 0.25 ?g per 10^6 cells in 100 ?L volume.
Tags: C1.18.4 isotype control mAb; C1.18.4 recombinant isotype control

Rat IgG2b Isotype Control (C1.18.4).
Not specified. (2025). Thermo Fisher Scientific Product Datasheet. doi: Not specified. PMID: Not specified
The monoclonal rat IgG2b, kappa (clone C1.18.4) serves as an isotype control immunoglobulin. It has been reported for use in immunohistochemistry, immunocytochemistry, flow cytometric analysis, and immunoglobulin ELISA. The antibody has been tested by flow cytometric analysis of mouse splenocyte suspension and should be used at the same concentration as the experimental antibody. Rat IgG2b is a subclass of immunoglobulin G commonly used in research and diagnostics, often as a negative control due to its lack of binding to many mammalian antigens.
Tags: C1.18.4 isotype control antibody for cancer research; C1.18.4 isotype control antibody for mouse tumor model

Purified Rat IgG2b Isotype Control (C1.18.4).
Not specified. (2025). Thermo Fisher Scientific Product Datasheet. doi: Not specified. PMID: Not specified
The monoclonal rat IgG2b, kappa (clone C1.18.4) is used as an isotype control immunoglobulin. It is reported for use in immunohistochemistry, immunocytochemistry, flow cytometric analysis, and immunoglobulin ELISA. The antibody has been tested by flow cytometric analysis of mouse splenocyte suspension and should be used at the same concentration as the experimental antibody. Rat IgG2b is a subclass of immunoglobulin G widely used in research and diagnostics, often as a negative control due to its lack of binding to many mammalian antigens.
Tags: C1.18.4 isotype control for cancer research; C1.18.4 isotype control for flow cytometry

Rat IgG2b Isotype Control (clone C1.18.4).
Not specified. (2025). Sigma-Aldrich Product Datasheet. doi: Not specified. PMID: Not specified
The Rat IgG2b Isotype Control (clone C1.18.4) is a non-reactive IgG2b monoclonal antibody used as an isotype-matched negative control for rat IgG2b antibodies. It is suitable for flow cytometry, immunohistochemistry, immunocytochemistry, and ELISA. The antibody was purified by protein G affinity chromatography from tissue culture supernatant and does not react with any known antigen. It is stable for 1 year at 2?8°C from the date of receipt.
Tags: C1.18.4 isotype control for mouse tumor model; function of C1.18.4 isotype control antibody

For more references about C1.18.4 isotype control antibodies please contact our scientific support team with message@sydlabs.com.

In Vivo Grade Recombinant Isotype Control Antibodies (Clone C1.18.4):
In vivo Grade Recombinant C1.18.4 Mouse IgG2a Isotype Control Antibody Mutants
In vivo Grade Recombinant C1.18.4 Mouse IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant C1.18.4 Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant C1.18.4 Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant C1.18.4 Mouse IgG3 Isotype Control Antibody and Mutants
In vivo Grade Recombinant C1.18.4 Mouse Fab Fragment
In vivo Grade Recombinant C1.18.4 Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant C1.18.4 Human IgG2 Isotype Control Antibody and Mutants
In vivo Grade Recombinant C1.18.4 Human IgG3 Isotype Control Antibody and Mutants
In vivo Grade Recombinant C1.18.4 Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant C1.18.4 Human Fab Fragment
In vivo Grade Recombinant C1.18.4 Human IgE Isotype Control Antibody
In vivo Grade Recombinant C1.18.4 Human IgM Isotype Control Antibody
In vivo Grade Recombinant C1.18.4 Human IgA1 Isotype Control Antibody
In vivo Grade Recombinant C1.18.4 Rat IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant C1.18.4 Rat IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant C1.18.4 Rat IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant C1.18.4 Rat IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant C1.18.4 Rat Fab Fragment
In vivo Grade Recombinant C1.18.4 Hamster IgG1 Isotype Control Antibody
In vivo Grade Recombinant C1.18.4 Hamster IgG2 Isotype Control Antibody
In vivo Grade Recombinant C1.18.4 Rabbit IgG Isotype Control Antibody
In vivo Grade Recombinant C1.18.4 Ferret IgG1 Isotype Control Antibody
In vivo Grade Recombinant C1.18.4 Ferret IgM Isotype Control Antibody
In vivo Grade Recombinant C1.18.4 Ferret IgA Isotype Control Antibody
In vivo Grade Recombinant C1.18.4 Canine IgG1 Isotype Control Antibody
In vivo Grade Recombinant C1.18.4 Canine IgG2 Isotype Control Antibody
In vivo Grade Recombinant C1.18.4 Canine IgG3 Isotype Control Antibody
In vivo Grade Recombinant C1.18.4 Canine IgG4 Isotype Control Antibody
In vivo Grade Recombinant C1.18.4 Feline IgG1 Isotype Control Antibody

Other Recombinant IgG Reference Antibodies:
In vivo Grade Recombinant Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Human IgG2 Isotype Control Antibody
In vivo Grade Recombinant Human IgG3 Isotype Control Antibody
In vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG3 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody