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Recombinant anti-HEL isotype control antibodies and Fc silenced mutants from clone HEL D1.3 good for in vitro and in vivo studies. Low or no specific binding to human, rat, and mouse samples tested. "Humanized" means the variable regions were humanized.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Background
The anti-HEL monoclonal antibody (hen egg-white lysozyme) in nature is suitable for use as an isotype-matched control antibody to estimate the degree of non-specific binding by an antigen-specific antibody. The HEL D1.3-based recombinant isotype control and mutants (natural and engineered isotype controls) are useful for in vitro and in vivo studies from ELISA, Western blot (WB), to cell-based assay to animal model study. As an ideal recombinant mouse IgG1 isotype control, the recombinant HEL D1.3 mIgG1 kappa isotype control has low or no specific binding to any mouse sample, and completely mouse variable regions and the mouse IgG1 kappa constant regions. The mouse IgG1 kappa isotype control and mutants can be used in ELISA, WB, the cell-based assay or animal model as the antibody negative control.
Please contact us to ask for a quote for the engineered HEL D1.3 isotype control mutants, isotype control and mutants with Avi-, His-, and Flag-tags, and biotinylated isotype control and mutants. A variety of conjugates (such as dyes and fluorophores) with the anti-HEL isotype control and mutants are available.
The in vivo grade recombinant HEL D1.3 mouse IgG1 isotype control antibody (mIgG1 isotype control) was produced in mammalian cells.
Immunogen: hen egg-white lysozyme (HEL).
Clone: HEL D1.3.
Isotype: mouse IgG1, kappa.
Applications: an isotype-matched negative control for mouse anti-HEL IgG1 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Shipping: The in vivo grade recombinant anti-HEL mouse IgG1 kappa isotype control antibody (clone of HEL D1.3) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.
In Vivo Grade Recombinant anti-HEL Isotype Control Antibodies (clone HEL D1.3):
In vivo Grade Recombinant HEL D1.3 Mouse IgG1 Isotype Control Antibody Mutants
In vivo Grade Recombinant HEL D1.3 Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant HEL D1.3 Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant HEL D1.3 Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant HEL D1.3 Mouse IgG3 Isotype Control Antibody and Mutants
In vivo Grade Recombinant HEL D1.3 Mouse Fab Fragment
In vivo Grade Recombinant HEL D1.3 Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant HEL D1.3 Human IgG2 Isotype Control Antibody and Mutants
In vivo Grade Recombinant HEL D1.3 Human IgG3 Isotype Control Antibody and Mutants
In vivo Grade Recombinant HEL D1.3 Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant HEL D1.3 Human Fab Fragment
In vivo Grade Recombinant HEL D1.3 Human IgE Isotype Control Antibody
In vivo Grade Recombinant HEL D1.3 Human IgM Isotype Control Antibody
In vivo Grade Recombinant HEL D1.3 Human IgA1 Isotype Control Antibody
In vivo Grade Recombinant HEL D1.3 Rat IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant HEL D1.3 Rat IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant HEL D1.3 Rat IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant HEL D1.3 Rat IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant HEL D1.3 Rat Fab Fragment
In vivo Grade Recombinant HEL D1.3 Hamster IgG1 Isotype Control Antibody
In vivo Grade Recombinant HEL D1.3 Hamster IgG2 Isotype Control Antibody
In vivo Grade Recombinant HEL D1.3 Rabbit IgG Isotype Control Antibody
In vivo Grade Recombinant HEL D1.3 Ferret IgG1 Isotype Control Antibody
In vivo Grade Recombinant HEL D1.3 Ferret IgM Isotype Control Antibody
In vivo Grade Recombinant HEL D1.3 Ferret IgA Isotype Control Antibody
In vivo Grade Recombinant HEL D1.3 Canine IgG1 Isotype Control Antibody
In vivo Grade Recombinant HEL D1.3 Canine IgG2 Isotype Control Antibody
In vivo Grade Recombinant HEL D1.3 Canine IgG3 Isotype Control Antibody
In vivo Grade Recombinant HEL D1.3 Canine IgG4 Isotype Control Antibody
In vivo Grade Recombinant HEL D1.3 Feline IgG1 Isotype Control Antibody
Other Recombinant IgG Reference Antibodies:
In vivo Grade Recombinant Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Human IgG2 Isotype Control Antibody
In vivo Grade Recombinant Human IgG3 Isotype Control Antibody
In vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG3 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody
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