BP000092-C017: Recombinant Human Leukemia Inhibitory Factor (LIF) Protein
Source: E. coli-derived.
The ED50 was determined by the M1 cell differentiation assay is < 0.01 ug/ml, corresponding to a specific activity of 1.0 x 108 IU/mg.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 IEU/ug).BP000092-CYT-001: Recombinant Human Leukemia Inhibitory Factor, GST tag
Source: E. coli-derived.
Purity: > 90% by SDS-PAGE and RP-HPLC.BP000092-CYT-644: Recombinant Human Leukemia Inhibitory Factor (LIF) Protein
Source: E. coli-derived.
The ED50 was determined by the M1 cell differentiation assay is < 0.01 ug/ml, corresponding to a specific activity of 100 MIU/mg.
Purity: > 98% by SDS-PAGE and RP-HPLC.BP000092-CYT-645: Recombinant Mouse Leukemia Inhibitory Factor (LIF) Protein
Source: E. coli-derived.
The specific activity is 100 MIU/mg, where 50 U is defined as the amount of murine LIF required to induce differentiation in 50% of the M1 colonies per ml of agar culture.
Purity: > 95% by SDS-PAGE and RP-HPLC.BP000092-GPF-001: Recombinant Murine Leukemia Inhibitory Factor (LIF) Protein, GST tag
Source: E. coli-derived.
mLIF supplement is assessed both on mESCs and on murine M1 myeloid leukemic cells. A standard of 50 units is defined as the concentration of mLIF supplement in 1.0 ml of tissue culture medium that induces the differentiation of 50% of M1 colonies (Metcalf, 1988).
Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/ml.
Murine myeloid leukemic M1 Assay: Specific Activity >108 units/mg.
Purity: > 95% by SDS-PAGE.