MB119-B: 2x hot-start high-fidelity PCR Master Mix, with blue dye
* Simple operation: The PCR master mix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination and pipetting errors. The tracking dye and precipitant have been added into the master mix so that the PCR product can be directly loaded for electrophoresis.
* Ultra-high fidelity: The fidelity is 53 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase. For every 300,000 bases amplified, there are fewer than 5 mismatches.
* Fast amplification: normally 4-5 sec/kb; ~0.5 sec/kb if the amplification length is <1 kb; 4-150 times that of conventional PCR master mix.
* Long fragment amplification: up to 40 kb from simple templates such as λDNA and plasmids, up to 20 kb from complex templates such as genomic DNA, and up to 10 kb from cDNA templates.
* Wide adaptability: It is suitable for the amplification of various GC content fragments, has super tolerance to PCR inhibitors, and can be used for direct PCR of a variety of samples.
* Features of the hot-start high fidelity DNA polymerase (catalog No. MB118) contained in the 2x hot-start high-fidelity PCR master mix.BP000472-PRO-407:Human Microglobulin Alpha-1 Protein (A1M)
Source: Purified from the urine of patients with chronic renal tubular proteinuria-derived.
Purity: > 96%.PA000633-C14262: ACBD6 Polyclonal Antibody
Rabbit Polyclonal Antibody;
Applications: WB, ELISA;
Reactivity: Human, Mouse, Rat.PA000633-R12-2434: ACBD6 Polyclonal Antibody
Rabbit Polyclonal Antibody;
Applications: WB, ELISA;
Reactivity: Human, Mouse, Rat.EK000081-EK0557: Human ACE ELISA Kit
Range: 156pg/ml-10,000pg/ml
Sensitivity < 5 pg/ml
Specificity: no detectable cross-reactivity with any other cytokine.
Application: for quantitive detection of human ACE in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: Four months at 4°C and eight months at -20°C.Our complete custom Adeno-associated Virus (AAV) production services include: 1) AAV plasmid vector construction; 2) AAV packaging; 3) AAV purification by iodixanol or column chromatography; 4) AAV titering by qPCR.
We have always optimized our AAV production systems, including AAV plasmid vector design and construction and low endotoxin plasmid preparation, host cells for AAV packaging, cell culture medium and AAV packaging process, small and large scale AAV purification process.
Fast turnaround: 2-3 weeks.Our Ad5-RGD and Ad5/35 adenovirus production services include: 1) construction of an Ad5-RGD and Ad5/35 adenovirus vector for expression or silencing of a gene; 2) generation of Ad5-RGD and Ad5/35 adenovirus; 3) amplification and purification of Ad5-RGD and Ad5/35 adenovirus.
Our adenovirus production team has constructed, amplified, and purified thousands of adenoviruses for worldwide clients in more than a decade. An improved technology is used to simplify and accelerate construction of adenoviral vectors for researchers studying gene expression or gene silencing in mammalian systems.
Fast turnaround: 2 weeks.BP002513-ENZ-595: Recombinant Human Alcohol Dehydrogenase 5 (ADH5) Enzyme
Source: E. coli-derived.
Purity: > 90% as determined by SDS-PAGE.BP000001-CYT-433: Recombinant Human Adiponectin (ADIPOQ), His tag Protein
Source: E. Coli-derived.
Purity: > 95% by SDS-PAGE.
Applications: WB.BP000001-CYT-434: Recombinant Human Adiponectin (ADIPOQ), HEK Protein
Source: HEK293 (Human Embryonic Kidney cell line)-derived.
In vitro gluconeogenesis assay in primary hepatocytes was performed, showing the Adiponectin human derived from mammalian cells can inhibit glucose production. The ED50 was ~6µg/ml.
Purity: > 98% by HPLC and SDS PAGE.
Applications: ELISA, WB, cell culture and/or animal studies.