BP005646: Biotinylated Human IL-1R3/IL-1 RAcP Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human IL-1R3/IL-1 RAcP Protein, (amino acids Ser21-Glu359) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Biotinylated Human IL-1R3 protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for the recombinant Anti-IL-1R3 Antibody protein with the C-hFc tag with the EC50 of 2.4 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP003172: Human IL-1R1 Protein with No Tag
Source: The recombinant Human IL-1R1 Protein, (amino acids Leu18-Thr332) fused with No Tag, was produced in HEK 293 cells.
Activity: The affinity constant of 11.37 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP004482: Human IL-1R1 Protein with the C-His Tag
Source: The recombinant Human IL-1R1 Protein, (amino acids Leu18-Thr332) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human IL-1R1 protein with the C-His tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human IL-1 Beta protein with the C-His tag with the EC50 of 0.13 ug/ml determined by ELISA (QC Test). The affinity constant of 0.51 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005501: Human IL-1R3/IL-1 RAcP Protein with the C-His-Avi Tag
Source: The recombinant Human IL-1R3/IL-1 RAcP Protein, (amino acids Ser21-Glu359) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human IL-1R3 protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Anti-IL-1R3 Antibody protein with the C-hFc tag with the EC50 of 17.4 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP004828: Human IL-1R3/IL-1 RAcP Protein with the C-hFc Tag
Source: The recombinant Human IL-1R3/IL-1 RAcP Protein, (amino acids Ser21-Glu359) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human IL-1R3 protein with the C-hFc tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Anti-IL-1R3 Antibody protein with the C-hFc tag with the EC50 of 18.6 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.PA004000-ANT-238: IL-1 Receptor Antagonist Monoclonal Antibody
Mouse Monoclonal Antibody;
Applications: WB, IP, ELISA;
Reactivity: Human;
Clone: NYR-hIL1RA;
Isotype: Mouse IgG2b.BP000144-C033: Recombinant Human Interleukin-1 Receptor Antagonist Protein (IL-1RA)
Source: E. coli-derived.
The ED50 as determined by the dose-dependant inhibition of IL-1 stimulation of D10S cells was found to be 0.5 ug/ml, corresponding to a Specific Activity of 2.0 x 106 IU/mg.
Purity: > 95% by RP-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 EU/ug).BP000144-CYT-203: Recombinant Human Interleukin-1 Receptor Antagonist Protein (IL-1RA)
Source: E. coli-derived.
The ED50 as determined by the dose-dependant inhibition of IL-1 stimulation of D10S cells was found to be 0.5 ug/ml corresponding to a Specific Activity of 2,000,000 IU/mg.
Purity: > 98% by SDS-PAGE and RP-HPLC.BP000144-CYT-481: Recombinant Human Interleukin-1 Receptor Antagonist Protein (IL-1RA), His Tag
Source: E. coli-derived.
Purity: > 95% by SDS-PAGE and RP-HPLC.BP000144-CYT-658: Recombinant Mouse Interleukin-1 Receptor Antagonist Protein (IL-1RA) Protein
Source: E. coli-derived.
The mouse IL-1Ra is capable of inhibiting IL-1-alpha activity in helper T cell line D10.G4.1.
Purity: > 98% by SDS-PAGE and RP-HPLC.BP000144-CYT-136: Recombinant Mouse Interleukin-1 Receptor Antagonist Protein (IL-1RA), His Tag
Source: E. coli-derived.
Purity: > 95% by SDS-PAGEBP000144-CYT-152: Recombinant Rat Interleukin-1 Receptor Antagonist Protein (IL-1RA)
Source: E. coli-derived.
Fully biologically active when compared to standard. Measured by its ability to inhibit IL1-dependent proliferation in D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 30-150 ng/ml (corresponding to a specific activity of 6,667-33,334 units/mg ) in the presence of 50 pg/ml of rrIL1.
Purity: > 95.0% by SDS-PAGE.