PA007128: In Vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody (hIgG4 Isotype Control)

The recombinant human IgG4-S228P isotype control antibody (hIgG4 S228P isotype control) was produced in CHO cells.
Applications: an isotype-matched negative control for human IgG4 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method. Endotoxin ≤0.05 EU/mg for PA007128.05, in vivo grade recombinant human IgG4-S228P isotype control antibody, premium grade (endotoxin ≤0.05 EU/mg).

PA007131: In Vivo Grade Recombinant Human IgG4-S228P L235E P329G Isotype Control Antibody (hIgG4 SPLEPG Isotype Control Mutant)

The recombinant human IgG4-S228P L235E P329G isotype control antibody (hIgG4 SPLEPG isotype control mutant, Fc silenced) was produced in mammalian cells.
Applications: an isotype-matched negative control for human IgG4 SPLEPG kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007331.m1: Non-conjugated Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8), Mouse IgG1 kappa

Recombinant  mouse IgG1 Monoclonal Antibody. 
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant mouse monoclonal antibody (clone: 3G8) specifically binds to human and non-human primate CD16.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 

PA007331.h1Fs: Non-conjugated Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8), Human IgG1 Fc Silent Kappa

Recombinant  human IgG1 Monoclonal Antibody with Fc silent mutation. 
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 3G8 antibody specifically binds to human and non-human primate CD16.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 

PA007332.h1Fs-b: Biotinylated Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8), Human IgG1 Fc Silent Kappa

Recombinant  human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain. 
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant 3G8 antibody specifically binds to human and non-human primate CD16.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.

PA007277.m1: In vivo Grade Recombinant Anti-human CD16 Monoclonal Antibody, Mouse IgG1 Kappa (Clone: 3G8)

Recombinant mouse IgG1 Monoclonal Antibody. 
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: 3G8) specifically binds to human and non-human primate CD16.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD16 protein.

BP003178: Human Fc gamma RIIIA/CD16a (V176) Domain 2 Protein with the C-mFc Tag

Source: The recombinant Human Fc gamma RIIIA/CD16a (V176) Domain 2 Protein, (amino acids Gly107-Thr189) fused with a mFc tag at the C-terminus, was produced in HEK 293 cells.
Activity:
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

PA007125: In Vivo Grade Recombinant Human IgG1 kappa Isotype Control Antibody

The in vivo grade recombinant human IgG1 isotype control antibody was produced in CHO cells.
Applications: an isotype-matched negative control for human IgG1 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method. Endotoxin ≤0.05 EU/mg for PA007125.05, in vivo grade recombinant human IgG1 isotype control antibody, premium grade (endotoxin ≤0.05 EU/mg).

PA007134: In Vivo Grade Recombinant Human IgG1-L234A L235A P329G (hIgG1 LALAPG) kappa Isotype Control Antibody

The in vivo grade recombinant human IgG1-L234A L235A P329G (hIgG1 LALAPG, Fc silenced) kappa isotype control mutant was produced in CHO cells.
Applications: an isotype-matched negative control for human IgG1 LALAPG kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method. Endotoxin ≤0.05 EU/mg for PA007134.05, in vivo grade recombinant human IgG1-L234A L235A P329G (hIgG1 LALAPG) isotype control antibody, premium grade (endotoxin ≤0.05 EU/mg).

PA007133: In Vivo Grade Recombinant Human IgG1-N297A kappa Isotype Control Antibody

The in vivo grade recombinant human IgG1-N297A kappa isotype control mutant was produced in mammalian cells.
Applications: an isotype-matched negative control for human IgG1 N297A kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007132: In Vivo Grade Recombinant Human IgG1-D265A kappa Isotype Control Antibody

The in vivo grade recombinant human IgG1-D265A kappa isotype control mutant was produced in mammalian cells.
Applications: an isotype-matched negative control for human IgG1 D265A kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007139: In Vivo Grade Recombinant Human IgG1-D265A N297A (hIgG1 DANA) kappa Isotype Control Antibody

The in vivo grade recombinant human IgG1-D265A N297A (hIgG1 DANA) kappa isotype control mutant was produced in mammalian cells.
Applications: an isotype-matched negative control for human IgG1 DANA kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007140: In Vivo Grade Recombinant Human IgG1-S267E L328F (hIgG1 SELF) kappa Isotype Control Antibody

The in vivo grade recombinant human IgG1-S267E L328F (hIgG1 SELF, Fc activated) kappa isotype control mutant was produced in mammalian cells.
Applications: an isotype-matched negative control for human IgG1 SELF kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

BP001445-C441: Recombinant Human Fc?RIIIA (FCGR3A) Protein

Source: HEK 293-derived.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 IEU/ug).

BP4099: Recombinant human Fc gamma RIIIA (V158) protein

Source: HEK 293 cell-derived.
Purity: >95% by SDS-PAGE under reducing conditions.
Activity: Recombinant human Fc gamma RIIIA (V158) protein binds to human IgG as a low affinity receptor.
Endotoxin Level: Less than 1 EU/ug of protein as determined by LAL method.

BP4098: Recombinant human Fc gamma RIIIA (F158) protein

Source: HEK 293 cell-derived.
Purity: >95% by SDS-PAGE under reducing conditions.
Activity: Recombinant human Fc gamma RIIIA (F158) protein binds to human IgG as a low affinity receptor.
Endotoxin Level: Less than 1 EU/ug of protein as determined by LAL method.

BP005444: Human Fc gamma RIIIA/CD16a (F176) Protein with the C-His-Avi Tag

Source: The recombinant Human Fc gamma RIIIA/CD16a (F176) Protein, (amino acids Gly17-Gln208(F176)) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human Fc gamma RIIIA (F176) protein with the C-His-Avi tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-Fc gamma RIIIA Antibody protein with the C-hFc tag with the EC50 of 4.4 ng/ml determined by ELISA (QC Test). The affinity constant of 0.92 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005710: Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein with the C-His-Avi Tag

Source: The recombinant Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein, (amino acids Gly17-Gln208(F176)) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Anti-Fc gamma RIIIA Antibody protein with the C-His-Avi tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Biotinylated Human Fc gamma RIIIA (F176) protein with the C-His-Avi tag with the EC50 of 35.7 ng/ml determined by ELISA (QC Test). The affinity constant of 0.14 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.