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Conditions of optimal FCGR3A Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
Receptors for the Fc region of immunoglobin G (Fc? R), also known as CD16 or Fc? R?, is divided into three classes and CD16 (Fc? RIII) is a multifunctional, low/intermediate affinity receptor. In humans, CD16 is expressed as two distinct forms (CD16a and CD16b) encoded by two different highly homologous genes in a cell type-specific manner. CD16b is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas CD16a is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed by a subset of T lymphocytes, natural killer (NK) cells, monocytes, and macrophages.The CD16a receptor is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), mast cell degranulation, and clearance of immune complexes. CD16a has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain and delivers an activation signal in the immune responses. Aberrant expression or mutations in this gene is implicated in susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia. In humans, it is a 50 -70 kDa type I transmembrane activating receptor. The Fc? RIIIa cDNA encodes 254 aa including a 16aa signal sequence, 191 aa ECD with two C2-type Ig-like domains and five potenti N-glycosylation sites, a 22 aa transmembrane (TM) sequence and a 25 aa cytoplasmic domain.
BP001445-C441: Recombinant Human Fc?RIIIA (FCGR3A) Protein
Source: HEK 293-derived.
Recombinant Human Fc?RIIIA (FCGR3A) Protein is a secreted protein with sequence (Gly17-Gln208) of Human FCGR3A with a polyhistidine tag at the C-terminus.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 IEU/ug)
Formulation: Lyophilized from a 0.2 um filtered solution of 20 mM PBS, 150 mM NaCl, pH 7.2.
Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
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