Fast turnaround: 3-6 months with our proprietary optimization algorithm.
Antibody affinity can be improved by about 10 folds after each round of antibody affinity maturation.
20000 cells per hybridoma clone are sufficient for cloning and sequencing the monoclonal antibody variable regions (both VH and VL) and full length. Welcome to challenge us with smaller number of clonal B cells or non-viable cells for hybridoma rescue.
Both Sanger sequencing-based and nanopore sequencing-based methods are available for antibody sequencing from hybridoma and clonal B cells. Sanger sequencing is labor intensive and time consuming so that it is difficult to reduce the cost for a large number of samples. However, nanopore sequencing can be used to reduce the cost signicantly, especially for a large number of samples.
De novo antibody sequencing to obtain the intact amino acid sequence of variable and/or constant regions of monoclonal antibodies by LC-MS/MS.
Sample requirement: 3-5 mg at 1 mg/ml preferred (minimum: 2 mg at 1 mg/mL).
Fast turnaround time: 2-3 months per antibody, but may require more time depending on the complexity of analysis.Syd Labs generates scFv phage libraries from immunized or naive donors from several species. Additionally, various mutagenesis strategies can be used to design scFv phage libraries for affinity maturation of a given antibody or other optimization purposes. Our scFv phage library is generated with a goal of up to 1010 clones and quality controlled by sequencing 96 clones at random.
The antibody variable regions of both heavy chain (VH) and light chain (VL) are analyzed for liabilities including, but not limited to N-linked glycosylation, deamidation, isomerization, pyroglutamate formation, and oxidation.
Fast turnaround time: 1 week per antibody, but may require more time depending on the complexity of analysis.Single B cell antibody sequencing has become popular for antibody discovery, especially for those difficult to obtain. After antigen specific single B cell sorting, the single B cells can be directly used for antibody seqencing or cultured for 2 weeks before antibody sequencing. Single B cell sequencing by next generation sequencing (NGS) including nanopore sequencing has been successfully applied to single B cell antibody technologies.