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Home > Antibodies > A-Z Primary Antibodies > DYKDDDDK-Tag Antibodies for Flow Cytometry

DYKDDDDK-Tag Antibodies for Flow Cytometry

the same epitope detected by the Sigma-Aldrich 'Anti-FLAG' M2 Antibody

Catalog No. Product Name Size List Price (US$) Quantity
Description

PA006833.m1: HRP Conjugated Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (clone L1.1), Mouse IgG1 Kappa

The recombinant anti-DYKDDDDK-Tag mouse IgG1 monoclonal antibody (clone L1.1) is produced in mammalian cells. It consists of mouse variable regions and mouse IgG1 kappa constant regions. 
Clone: L1.1.
Isotype: mouse IgG1 kappa.
Conjugation: HRP-conjugated.
Specificity/Sensitivity: detection and purification of DYKDDDDK fusion proteins no matter if the DYKDDDDK sequence is present at the N-terminus, Met N-terminus, C-terminus, or an internal site of the fusion proteins. The antibody recognizes the same epitope as the 'Anti-FLAG' M2 antibody detects.
Applications: ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation, electron microscopy, supershift assays, protein purification if conjugated with agarose or magnetic beads, and isotype controls. 
Reactivity: Human, Mouse, Rat, Others.
Purification: The DYKDDDDK-tag antibody was affinity-purified from supernatant of mammalian cells cultured in a chemically defined medium by affinity-chromatography using protein G.
Form of Antibody: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.

PA006834.m1: FITC Conjugated Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (clone L1.1), Mouse IgG1 Kappa

The recombinant anti-DYKDDDDK-Tag mouse IgG1 monoclonal antibody (clone L1.1) is produced in mammalian cells. It consists of mouse variable regions and mouse IgG1 kappa constant regions. 
Clone: L1.1.
Isotype: mouse IgG1 kappa.
Conjugation: FITC-conjugated.
Specificity/Sensitivity: detection and purification of DYKDDDDK fusion proteins no matter if the DYKDDDDK sequence is present at the N-terminus, Met N-terminus, C-terminus, or an internal site of the fusion proteins. The antibody recognizes the same epitope as the 'Anti-FLAG' M2 antibody detects.
Applications: ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation, electron microscopy, supershift assays, protein purification if conjugated with agarose or magnetic beads, and isotype controls. 
Reactivity: Human, Mouse, Rat, Others.
Purification: The DYKDDDDK-tag antibody was affinity-purified from supernatant of mammalian cells cultured in a chemically defined medium by affinity-chromatography using protein G.
Form of Antibody: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.

PA006835.m1: IFluor 488 Conjugated Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (clone L1.1), Mouse IgG1 Kappa

The recombinant anti-DYKDDDDK-Tag mouse IgG1 monoclonal antibody (clone L1.1) is produced in mammalian cells. It consists of mouse variable regions and mouse IgG1 kappa constant regions. 
Clone: L1.1.
Isotype: mouse IgG1 kappa.
Conjugation: iFluor 488-conjugated.
Specificity/Sensitivity: detection and purification of DYKDDDDK fusion proteins no matter if the DYKDDDDK sequence is present at the N-terminus, Met N-terminus, C-terminus, or an internal site of the fusion proteins. The antibody recognizes the same epitope as the 'Anti-FLAG' M2 antibody detects.
Applications: ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation, electron microscopy, supershift assays, protein purification if conjugated with agarose or magnetic beads, and isotype controls. 
Reactivity: Human, Mouse, Rat, Others.
Purification: The DYKDDDDK-tag antibody was affinity-purified from supernatant of mammalian cells cultured in a chemically defined medium by affinity-chromatography using protein G.
Form of Antibody: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.

PA006836.m1: APC Conjugated Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (clone L1.1), Mouse IgG1 Kappa

The recombinant anti-DYKDDDDK-Tag mouse IgG1 monoclonal antibody (clone L1.1) is produced in mammalian cells. It consists of mouse variable regions and mouse IgG1 kappa constant regions. 
Clone: L1.1.
Isotype: mouse IgG1 kappa.
Conjugation: iFluor 488-conjugated.
Specificity/Sensitivity: detection and purification of DYKDDDDK fusion proteins no matter if the DYKDDDDK sequence is present at the N-terminus, Met N-terminus, C-terminus, or an internal site of the fusion proteins. The antibody recognizes the same epitope as the 'Anti-FLAG' M2 antibody detects.
Applications: ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation, electron microscopy, supershift assays, protein purification if conjugated with agarose or magnetic beads, and isotype controls. 
Reactivity: Human, Mouse, Rat, Others.
Purification: The DYKDDDDK-tag antibody was affinity-purified from supernatant of mammalian cells cultured in a chemically defined medium by affinity-chromatography using protein G.
Form of Antibody: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.

PA006837.m1: PE Conjugated Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (clone L1.1), Mouse IgG1 Kappa

The recombinant anti-DYKDDDDK-Tag mouse IgG1 monoclonal antibody (clone L1.1) is produced in mammalian cells. It consists of mouse variable regions and mouse IgG1 kappa constant regions. 
Clone: L1.1.
Isotype: mouse IgG1 kappa.
Conjugation: PE-conjugated.
Specificity/Sensitivity: detection and purification of DYKDDDDK fusion proteins no matter if the DYKDDDDK sequence is present at the N-terminus, Met N-terminus, C-terminus, or an internal site of the fusion proteins. The antibody recognizes the same epitope as the 'Anti-FLAG' M2 antibody detects.
Applications: ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation, electron microscopy, supershift assays, protein purification if conjugated with agarose or magnetic beads, and isotype controls. 
Reactivity: Human, Mouse, Rat, Others.
Purification: The DYKDDDDK-tag antibody was affinity-purified from supernatant of mammalian cells cultured in a chemically defined medium by affinity-chromatography using protein G.
Form of Antibody: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The recombinant anti-DYKDDDDK-Tag monoclonal antibody (clone L1.1) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap.
1 month from date of receipt, 2 to 8°C as supplied.
12 months from date of receipt, -20°C to -70°C as supplied.

Other DYKDDDDK Antibodies:
Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (clone L1.1), Mouse IgG1 Kappa
Recombinant Anti-DYKDDDDK-Tag Mouse Fab Fragment (Clone L1.1)
Recombinant Anti-DYKDDDDK-Tag scFv His Tag (clone L1.1)
Recombinant Anti-DYKDDDDK-Tag Poly Mouse Fab (clone L1.1)
Recombinant Anti-DYKDDDDK-Tag Poly scFv (clone L1.1)
Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (Clone L1.1),Human lgG1 Kappa
Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (Clone L1.1), Rabbit IgG

Biotinylated DYKDDDDK Antibodies:
Biotinylated Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (Clone L1.1), Human IgG1 Fc Silent Kappa
Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (Clone L1.1),Human IgG1 Fc Silent Kappa-AviTag
Biotinylated Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (clone L1.1), Mouse IgG1 Kappa

DYKDDDDK Antibodies Conjugated with Agarose or Magnetic Microbead:
DYKDDDDK-tag Monoclonal Antibody (Magnetic Microbead-Conjugated)
DYKDDDDK-tag Monoclonal Antibody (Agarose-Conjugated)

DYKDDDDK Antibodies for Flow Cytometry:
HRP Conjugated Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (clone L1.1), Mouse IgG1 Kappa
FITC Conjugated Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (clone L1.1), Mouse IgG1 Kappa
IFluor 488 Conjugated Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (clone L1.1), Mouse IgG1 Kappa
APC Conjugated Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (clone L1.1), Mouse IgG1 Kappa
PE Conjugated Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (clone L1.1), Mouse IgG1 Kappa

DYKDDDDK Peptide:
DYKDDDDK Peptide

Other Tag Antibodies:
HA-Tag Monoclonal Antibody (Clone 12CA5)
His-Tag Monoclonal Antibody (Clone 3D5)
Myc-Tag Monoclonal Antibody (Clone 9E10)
His-tag Monoclonal Antibody (Clone HIS.H8)
GFP-tag Monoclonal Antibody
RFP-tag Monoclonal Antibody
His-Tag Monoclonal Antibody (Clone 26D11)
V5-tag Monoclonal Antibody (Clone V5.E3)

Loading Control Antibodies:
GAPDH Monoclonal Antibody
beta-Actin Monoclonal Antibody
beta-Tubulin Monoclonal Antibody

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