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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant His-Tag monoclonal antibodies (clone 3D5) with various isotypes; low prices for bulk order. Conditions of optimal His-Tag antibody (3D5) performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
PA007282.m2b: Recombinant Anti-His-Tag Monoclonal Antibody (clone 3D5), Mouse IgG2b Kappa
The recombinant Anti-His-Tag mouse IgG2b monoclonal antibody (clone 3D5) is produced in mammalian cells. It consists of rat variable regions and mouse IgG2b kappa constant regions.
Clone: 3D5.
Isotype: mouse IgG2b kappa.
Specificity/Sensitivity: His tagged proteins and His-tag expression cells. The 3D5 antibody recognizes the C-terminal carboxylate group of the hexahistidine peptide, the main chain of the last four His residues, and the last three imidazole side-chains.
Applications: ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation, electron microscopy, supershift assays, protein purification if conjugated with agarose or magnetic beads, and isotype controls.
Reactivity: Human, Mouse, Rat, Others.
Purification: The His-Tag antibody was affinity-purified from supernatant of mammalian cells cultured in a chemically defined medium by affinity-chromatography using protein A.
Form of Antibody: 1 mg/ml in 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The recombinant Anti-His-Tag mouse IgG2b monoclonal antibody (clone 3D5) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap.
1 month from date of receipt, 2 to 8°C as supplied.
12 months from date of receipt, -20°C to -70°C as supplied.
Other Tag Antibodies:
DYKDDDDK-tag Monoclonal Antibody (Clone M2.1)
HA-Tag Monoclonal Antibody (Clone 12CA5)
Myc-Tag Monoclonal Antibody (Clone 9E10)
His-tag Monoclonal Antibody (Clone HIS.H8)
GFP-tag Monoclonal Antibody
RFP-tag Monoclonal Antibody
His-Tag Monoclonal Antibody (Clone 26D11)
V5-tag Monoclonal Antibody (Clone V5.E3)
Loading Control Antibodies:
GAPDH Monoclonal Antibody
beta-Actin Monoclonal Antibody
beta-Tubulin Monoclonal Antibody
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