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Capillary Electrophoresis-SDS Services

CE-SDS
Catalog No. Product Name Size List Price (US$) Quantity
BS058A CE-SDS analysis, one sample, reducing conditions 1 sample 75.00
BS058B CE-SDS Analysis, Non-reducing Conditions 1 sample 75.00
BS058C Profiling of Heavy Chain Glycosylation, Reducing Conditions 1 sample 399.00
BS058D Profiling of Heavy Chain Glycosylation, Non-reducing Conditions 1 sample 399.00
Description

Microchip capillary electrophoresis-based separation (Microchip CE-SDS, PerkinElmer LabChip GXII system) provides information on approximate protein size, purity and homogeneity of a given protein sample. It is evaluated as high-throughput alternative to conventional CE-SDS and SDS-PAGE for monitoring monoclonal antibody protein quality with minimal sample preparation artifacts.

The principle is based on traditional gel electrophoresis that has been transferred to a chip format. Microchip CE-SDS based separation techniques offer several advantages over conventional electrophoretic separation methods, including high resolution/efficiency/speed, reproducibility and enhanced quantitation capabilities.approximate protein size, purity, and homogeneity of a given protein sample. It is evaluated as high-throughput alternative to conventional CE-SDS and SDS-PAGE for monitoring monoclonal antibody protein quality with minimal sample preparation artifacts.

Deliverable information:
- Report with approximate molecular weight, purity/impurity profiles of reduced or non-reduced protein samples.
- Ratio between HC and LC.
- Determination of glycosylated modification in heavy chain by comparisons of deglycosylated and non-treatment antibodies.

Sample treatment: Denaturation (reducing & non-reducing) followed by separation on a gel chip.

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