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Antibodies
Primary antibodies, monoclonal and polyclonal antibodies, and secondary antibodies for Western blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF), and Flow Cytometry (FC or Flow).
- IA000032-1008: Biotin-Capture Beads
The Biotin-Capture Beads contain modified Avidin that is immobilized on rigid, highly cross-linked beaded agarose possessing high chemical Stability. The modified Avidin provides a substantial improvement over native Avidin. The high affinity to biotin is maintained, while background problems are minimized due to chemical modification on the protein. The Biotin-Capture Beads has proven to be particularly useful in the isolation of antigens and nucleic acids by employing biotinylated antibodies, or nucleic acid probes respectively. Biotin-Capture Beads can be used in numerous additional affinity chromatography applications. - IA000033-1009
The Mono-Avidin Beads enables mild affinity purification of biotinylated proteins, peptides and other ligands. The mono-avidin molecules immobilized on the beads have a much lower biotin-binding affinity than native avidin thus enabling dissociation of biotinylated molecules using free biotin molecules or low pH buffer. In addition, the Mono-Avidin Beads can be regenerated at least 10 times with no significant loss in binding capacity. - IA000034-1011: Imino-Biotin Beads
The Imino-Biotin Beads enables mild affinity purification of various proteins belonging to the Avidins family. The Imino-Biotin molecules immobilized on the beads are capable of binding Avidins at neutral and basic pH and eluting in under acidic conditions. The Imino-Biotin Beads can be regenerated at least 10 times with no significant loss in binding capacity. - IA000035-1031
Goat Anti-Rabbit IgG (whole molecule) was affinity purified on Rabbit IgG affinity column using IgG isolated from pooled normal Rabbit sera. Eluted antibodies were further purified by Protein G column in order to separate IgGs from other classes of antibodies. Purified Antibodies were coupled to Sepharose Beads using TargetLock Chemistry. - IA000036-1032
Goat Anti-Mouse IgG (whole molecule) was affinity purified on Mouse IgG affinity column using IgG isolated from pooled normal Mouse sera. Eluted antibodies were further purified by Protein G column in order to separate IgGs from other classes of antibodies. Purified Antibodies were coupled to Sepharose Beads using TargetLock Chemistry. - IA000037-1029
Polyacrylamide gel electrophoresis (PAGE) has proven to be a useful tool for the separation of protein subunits and the determination of their molecular masses. Biotinylated Protein Size-Markers enables detection of size markers through biotin. For example HRP-Streptavidin may be used for the detection of the marker bands on blot or on film. The proteins included in Biotinylated Protein Size-Markers provide a suitable molecular mass range for most applications. Staining markers with Brilliant Blue R stain is possible. Protein bands are approximately equal in color intensity. - IA000039-A-9002-001
HRP-IgG conjugates in just over 4 hr
Includes columns to easily deliver purified conjugates
Gentle chemistry: Leaves antibodies intact, no reducing reagents
Easy-to-use protocols and calculators on flash drive
Compatible with Western, ELISA and IHC applications
This is the only kit on the market that can guarantee 100% conversion of antibody to conjugate, a purified conjugate free of any unlabeled antibody or enzyme and a high final yield of > 50% - IA000040-M-1002-010
The high surface area and lower non-specific binding of NanoLink Streptavidin Magnetic Beads are ideal for immobilizations and Co-IP applications. These microspheres are particularly suited for high throughput robotic applications where high biotin loads must be removed or immobilized without the presence of an iron leachate.
IA000040-M-1003-010
The high surface area and lower non-specific binding of MagnaLink Streptavidin Magnetic Beads are ideal for immobilizations and Co-IP applications. These microspheres are particularly suited for high throughput robotic applications where high biotin loads must be removed or immobilized without the presence of an iron leachate. - IA000041-M-1002-010
The high surface area and lower non-specific binding of NanoLink? Streptavidin Magnetic Beads are ideal for immobilizations and Co-IP applications. These microspheres are particularly suited for high throughput robotic applications where high biotin loads must be removed or immobilized without the presence of an iron leachate. - IA000042-A-9105-001
AP-IgG conjugates in just over 4 hr
Includes columns to easily deliver purified conjugates
Gentle chemistry: Leaves antibodies intact ? No reducing reagents
Easy-to-use protocols and calculators on flash drive
Compatible with Western, ELISA and IHC applications
This is the only kit on the market that can guarantee 100% conversion of antibody to conjugate, a purified conjugate free of any unlabeled antibody or enzyme and a high final yield of > 50%
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