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Antibodies
Primary antibodies, monoclonal and polyclonal antibodies, and secondary antibodies for Western blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF), and Flow Cytometry (FC or Flow).
- IA000043-P-9002-002
R_PE_antibody bioconjugation technology is superior to the maleimido/thiol_based method as it is:
More efficient: Greater than 95% of antibody is converted to conjugate.
More easily purified: In most cases, the percent conversion of free antibody to conjugate is 100%, leaving no unconjugated antibody. Therefore it is only necessary to purify out the excess R_PE to obtain a purified conjugate. In many applications even, purification is not necessary.
Controllable: The level of polymerization, and therefore the brightness of the conjugate, can be controlled by adjusting the level of HyNic_modification on the antibody. In flow cytometry applications, heterodimer product is required and preparation of this construct can be easily optimized using this technology.
Gentle on Antibodies: Other conjugation methods expose thiols on antibodies by DTT reduction of disulfide bonds, which cleaves the antibody into a variety of species. Our technology, however, gently incorporates HyNic moieties on the intact antibody. - IA000046-S-9002-1
The S-HyNic Conjugation Kit can be used to covalently crosslink any two amine containing biomolecules - IA000049-S-9003-1
Low price, high quality - IA000050-S-9001-1
SHNH (HyNic) is a heterobifunctional linker that reacts with primary amines on biomolecules and converts them to 4-HyNic linker groups - IA000051-S-3034
6-Fmoc-Lysine-(6-Boc-HyNic) is used to incorporate HyNic (6-hydrazinonicotinamide) moieties on the C-terminus and internal positions during solid or solution phase peptide synthesis - IA000052-HL-1002
Used for incorporation of HyNic linkers on silica/glass surfaces - IA000054-S-1005-250
Direct incorporation of 4FB (4-formylbenzamide) moieties on oligonucleotides during solid phase synthesis - IA000058-HL-1004-025
Used for incorporation of 4FB linkers on silica/glass surfaces - IA000060-S-1001-010
SHNH (Succinimidyl 6-hydraziniumnicotinate hydrochloride) is used to incorporate HyNic (hydrazinonicotinamide) moieties on proteins, peptides and other amine containing molecules for technetium-99M labeling. - IA000061-S-1009-010
MHPH is used to convert thiol moieties on biomolecules and surfaces to HyNic (6-hydrazinonicotinamide) moieties in a single step. Its advantages include reaction specificity, UV-traceability, and the unique control it brings to the entire conjugation process
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