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Antibodies
Primary antibodies, monoclonal and polyclonal antibodies, and secondary antibodies for Western blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF), and Flow Cytometry (FC or Flow).
- IA000037-1029
Polyacrylamide gel electrophoresis (PAGE) has proven to be a useful tool for the separation of protein subunits and the determination of their molecular masses. Biotinylated Protein Size-Markers enables detection of size markers through biotin. For example HRP-Streptavidin may be used for the detection of the marker bands on blot or on film. The proteins included in Biotinylated Protein Size-Markers provide a suitable molecular mass range for most applications. Staining markers with Brilliant Blue R stain is possible. Protein bands are approximately equal in color intensity. - IA000039-A-9002-001
HRP-IgG conjugates in just over 4 hr
Includes columns to easily deliver purified conjugates
Gentle chemistry: Leaves antibodies intact, no reducing reagents
Easy-to-use protocols and calculators on flash drive
Compatible with Western, ELISA and IHC applications
This is the only kit on the market that can guarantee 100% conversion of antibody to conjugate, a purified conjugate free of any unlabeled antibody or enzyme and a high final yield of > 50% - IA000040-M-1002-010
The high surface area and lower non-specific binding of NanoLink Streptavidin Magnetic Beads are ideal for immobilizations and Co-IP applications. These microspheres are particularly suited for high throughput robotic applications where high biotin loads must be removed or immobilized without the presence of an iron leachate.
IA000040-M-1003-010
The high surface area and lower non-specific binding of MagnaLink Streptavidin Magnetic Beads are ideal for immobilizations and Co-IP applications. These microspheres are particularly suited for high throughput robotic applications where high biotin loads must be removed or immobilized without the presence of an iron leachate. - IA000041-M-1002-010
The high surface area and lower non-specific binding of NanoLink? Streptavidin Magnetic Beads are ideal for immobilizations and Co-IP applications. These microspheres are particularly suited for high throughput robotic applications where high biotin loads must be removed or immobilized without the presence of an iron leachate. - IA000042-A-9105-001
AP-IgG conjugates in just over 4 hr
Includes columns to easily deliver purified conjugates
Gentle chemistry: Leaves antibodies intact ? No reducing reagents
Easy-to-use protocols and calculators on flash drive
Compatible with Western, ELISA and IHC applications
This is the only kit on the market that can guarantee 100% conversion of antibody to conjugate, a purified conjugate free of any unlabeled antibody or enzyme and a high final yield of > 50% - IA000043-P-9002-002
R_PE_antibody bioconjugation technology is superior to the maleimido/thiol_based method as it is:
More efficient: Greater than 95% of antibody is converted to conjugate.
More easily purified: In most cases, the percent conversion of free antibody to conjugate is 100%, leaving no unconjugated antibody. Therefore it is only necessary to purify out the excess R_PE to obtain a purified conjugate. In many applications even, purification is not necessary.
Controllable: The level of polymerization, and therefore the brightness of the conjugate, can be controlled by adjusting the level of HyNic_modification on the antibody. In flow cytometry applications, heterodimer product is required and preparation of this construct can be easily optimized using this technology.
Gentle on Antibodies: Other conjugation methods expose thiols on antibodies by DTT reduction of disulfide bonds, which cleaves the antibody into a variety of species. Our technology, however, gently incorporates HyNic moieties on the intact antibody. - IA000046-S-9002-1
The S-HyNic Conjugation Kit can be used to covalently crosslink any two amine containing biomolecules - IA000049-S-9003-1
Low price, high quality - IA000050-S-9001-1
SHNH (HyNic) is a heterobifunctional linker that reacts with primary amines on biomolecules and converts them to 4-HyNic linker groups - IA000051-S-3034
6-Fmoc-Lysine-(6-Boc-HyNic) is used to incorporate HyNic (6-hydrazinonicotinamide) moieties on the C-terminus and internal positions during solid or solution phase peptide synthesis
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