BP005447: Human PD-1/PDCD1 Protein with the C-His Tag
Source: The recombinant human PD-1/PDCD1 protein, the PD1 ECD (amino acids Leu25-Gln167) fused with a His-tag at the C-terminus, was produced in HEK 293 cells.
Activity: The recombinant human PD-L1 with the C-hFc Tag captured on CM5 Chip via Protein A can bind to the recombinant human PD-1 with the C-His Tag with an affinity constant of 2.2 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
BP005354: Human PD-1/PDCD1 Protein with the C-hFc Tag
Source: The recombinant human PD-1/PDCD1 protein, the PD1 ECD (amino acids Leu25-Gln167) fused with a hFc at the C-terminus, was produced in HEK 293 cells.
Activity: The EC50 of the recombinant human PD-L1 protein with the mFc tag to the immobilized human PD-1/PDCD1 with the C-hFc tag at 1 ug/ml (100 ul/Well) was 0.1 ug/ml determined by ELISA. The recombinant human PD-1 with the C-hFc Tag captured on CM5 Chip via Protein A can bind to the biotinylated recombinant human PD-L2 with the C-His-Avi Tag with an affinity constant of 0.344 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
BP005376: Human PD-1/PDCD1 Protein with the C-mFc Tag
Source: The recombinant human PD-1/PDCD1 protein, the PD1 ECD (amino acids Leu25-Gln167) fused with a mFc at the C-terminus, was produced in HEK 293 cells.
Activity: The EC50 of the biotinylated anti-human PD-1 antibody with the hFc tag to the immobilized human PD-1/PDCD1 protein with the mFc tag at 0.5 ug/ml (100 ul/Well) was 68 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.