BP005345: Human PD-L1/B7-H1 Protein with the C-His Tag
Source: The recombinant human PD-L1/B7-H1 protein, the PD-L1 ECD (amino acids Phe19-Arg238) fused with a His-tag at the C-terminus, was produced in HEK 293 cells.
Activity: The EC50 of the anti-human PD-L1 antibody with the hFc tag to the immobilized human PD-L1/B7-H1 protein with the His tag at 0.5 ug/ml (100 ul/Well) was 6.4 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
BP003570: Human PD-L1/B7-H1 Protein with the C-His Tag
Source: The recombinant human PD-L1/B7-H1 protein, the PD-L1 ECD (amino acids Phe19-Ile226) fused with a His-tag at the C-terminus, was produced in HEK 293 cells.
Activity: The EC50 of the anti-human PD-L1 antibody with the hFc tag to the immobilized human PD-L1/B7-H1 protein with the His tag at 0.5 ug/ml (100 ul/Well) was 4.3 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
BP004986: Human PD-L1/B7-H1 Protein with the C-hFc Tag
Source: The recombinant human PD-L1/B7-H1 protein, the PD-L1 ECD (amino acids Phe19-Arg238) fused with a hFc at the C-terminus, was produced in HEK 293 cells.
Activity: The EC50 of the recombinant human PD-L1 protein with the hFc tag to the immobilized human PD-1/PDCD1 with the C-mFc tag at 1 ug/ml (100 ul/Well) was 0.17 ug/ml determined by ELISA. The recombinant human PD-L1 with the C-hFc Tag captured on CM5 Chip via Protein A can bind to the recombinant human PD-1 with the C-His Tag with an affinity constant of 2.20 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
BP005315: Human PD-L1/B7-H1 Protein with the C-mFc Tag
Source: The recombinant human PD-L1/B7-H1 protein, the PD-L1 ECD (amino acids Phe19-Arg238) fused with a mFc at the C-terminus, was produced in HEK 293 cells.
Activity: The EC50 of the anti-human PD-L1 antibody with the hFc tag to the immobilized human PD-L1/B7-H1 protein with the C-mFc tag at 0.5 ug/ml (100 ul/Well) was 6.1 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP004753: Biotinylated Cynomolgus/Rhesus macaque PD-L1/B7-H1 Protein with the C-His Tag
Source: The recombinant cynomolgus/rhesus macaque PD-L1/B7-H1 protein, the PD-L1 ECD (amino acids Phe19-Arg238) fused with a His-tag at the C-terminus, was produced in HEK 293 cells.
Activity: The EC50 of the anti-cynomolgus PD-L1 antibody with the hFc tag to the immobilized biotinylated cynomolgus/rhesus macaque PD-L1/B7-H1 protein with the His tag at 1 ug/ml (100 ul/Well) was 4.0 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.