IA123: NMab Protein G Resin
* Cost competitive, high binging capacity alkaline-stable protein G resins.
* High dynamic binding capacity: 55 - 65 mg/mL (human IgG, 5 minute residence time).
* Clean in Place: 6 M guanidine hydrochloride.
BP003700: Cynomolgus PD-1/PDCD1 Protein with the C-His Tag
Source: The recombinant cynomolgus PD-1/PDCD1 protein, the PD1 ECD (amino acids Pro21-Gln167) fused with a His-tag at the C-terminus, was produced in HEK 293 cells.
Activity: The EC50 of the biotinylated anti-cynomolgus PD-1 antibody with the hFc tag to the immobilized cynomolgus PD-1/PDCD1 protein with the His tag at 2 ug/ml (100 ul/Well) was 4.1 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
BP005346: Cynomolgus PD-1/PDCD1 Protein with the C-hFc Tag
Source: The recombinant cynomolgus PD-1/PDCD1 protein, the PD1 ECD (amino acids Pro21-Gln167) fused with a hFc at the C-terminus, was produced in HEK 293 cells.
Activity: The EC50 of the biotinylated anti-cynomolgus PD-1 antibody with the hFc tag to the immobilized cynomolgus PD-1/PDCD1 with the C-hFc tag at 0.5 ng/ml (100 ul/Well) was 29.5 ng/ml determined by ELISA. The recombinant cynomolgus PD-1 with the C-hFc Tag captured on CM5 Chip via Protein A can bind to the biotinylated recombinant cynomolgus PD-L1 with the C-His-Avi Tag with an affinity constant of 1.28 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP003202: Canine PD-1/PDCD1 Protein with the C-hFc Tag
Source: The recombinant canine PD-1/PDCD1 protein, the PD1 ECD (amino acids Leu25-Val170) fused with a hFc at the C-terminus, was produced in HEK 293 cells.
Activity: The EC50 of the recombinant human PD-L1 protein with the mFc tag to the immobilized canine PD-1/PDCD1 with the C-hFc tag at 1 ug/ml (100 ul/Well) was 30.7 ng/ml determined by ELISA. The recombinant canine PD-1 with the C-hFc Tag captured on CM5 Chip via Protein A can bind to the recombinant human PD-L1 with the C-His Tag with an affinity constant of 0.696 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.