BP005275: Mouse PD-L1/B7-H1 Protein with the C-His Tag
Source: The recombinant mouse PD-L1/B7-H1 protein, the PD-L1 ECD (amino acids Phe19-Thr238) fused with a His-tag at the C-terminus, was produced in HEK 293 cells.
Activity: The recombinant mouse PD-L1 with the C-His Tag captured on CM5 Chip via the anti-His antibody can bind to the recombinant mouse PD-1 protein with the C-hFc Tag with an affinity constant of 0.62 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
BP005274: Mouse PD-L1/B7-H1 Protein with the C-hFc Tag
Source: The recombinant mouse PD-L1/B7-H1 protein, the PD-L1 ECD (amino acids Phe19-Arg237) fused with a hFc at the C-terminus, was produced in HEK 293 cells.
Activity: The recombinant human PD-1 with the C-His Tag captured on CM5 Chip via the anti-His antibody can bind to the recombinant mouse PD-L1 protein with the C-hFc Tag with an affinity constant of 92.49 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005535: Biotinylated Human PD-L1/B7-H1 Protein with the C-His Tag
Source: The recombinant human PD-L1/B7-H1 protein, the PD-L1 ECD (amino acids Phe19-Arg238) fused with a His-tag at the C-terminus, was produced in HEK 293 cells.
Activity: The EC50 of the biotinylated human PD-L1/B7-H1 protein with the His tag to the immobilized anti-human PD-L1 antibody with the hFc tag at 0.5 ug/ml (100 ul/Well) was 9.4 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.