BP005447: Human PD-1/PDCD1 Protein with the C-His Tag
Source: The recombinant Human PD-1/PDCD1 Protein, (amino acids Leu25-Gln167) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human PD-1 protein with the C-His tag at 1 ug/ml (100 ul/Well). Dose response curve for the recombinant Anti-PD-1 Antibody protein with the C-hFc tag with the EC50 of 6.9 ng/ml determined by ELISA (QC Test). The affinity constant of 2.20 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005354: Human PD-1/PDCD1 Protein with the C-hFc Tag
Source: The recombinant Human PD-1/PDCD1 Protein, (amino acids Leu25-Gln167) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human PD-L1 protein with the C-hFc tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human PD-1 protein with the C-hFc tag with the EC50 of 90.5 ng/ml determined by ELISA (QC Test). The affinity constant of 0.344 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005376: Human PD-1/PDCD1 Protein with the C-mFc Tag
Source: The recombinant Human PD-1/PDCD1 Protein, (amino acids Leu25-Gln167) fused with a mFc tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human PD-1 protein with the C-mFc tag at 0.5 ug/ml (100 ul/Well). Dose response curve for the recombinant Biotinylated Anti-PD-1 Ab protein with the C-hFc tag with the EC50 of 68 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005136: Mouse PD-1/PDCD1 Protein with the C-hFc Tag
Source: The recombinant Mouse PD-1/PDCD1 Protein, (amino acids Leu25-Gln167) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Activity: The affinity constant of 0.62 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.