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Home > Molecular Biology
Reagents and equipment for molecular biology, including synthesis, detection, identification, separation, purification, production, quantification, and modification of oligonucleotides, RNA, DNA, peptides, and proteins.
    70 Molecular Biology Products
  • MB127-5G: 5G RT Supermix for qPCR with gDNA Elimination Function

    * The 5G RT supermix for qPCR with gDNA elimination function is suitable for two-step RT-qPCR detection, and compatible with dye-based and probe-based qPCR, enabling high-performance gene expression analysis.
    * Simple and fast operation: one-step genome clearance and reverse transcription.
    * Higher reverse transcription efficiency and higher cDNA yields from all regions of RNA transcripts: based on the more efficient 5G Reverse Transcriptase.
    * Flexible choice of primers: Different types of reverse transcription primers can be used flexibly for different experimental designs.
    * Excellent cDNA stability: complete deactivation of thermal DNase and long-term storage of cDNA.
     

    MB127-4G: 4G RT Supermix for qPCR with gDNA Elimination Function

    * The 4G RT supermix for qPCR with gDNA elimination function is suitable for two-step RT-qPCR detection, and compatible with dye-based and probe-based qPCR, enabling high-performance gene expression analysis.
    * Based on the highly efficient 4G Reverse Transcriptase, the super temperature tolerance ensures that the complex secondary structure of RNA can be opened under high temperature conditions to obtain longer cDNA.
    * Quick and complete removal of genome contamination by the 4 × gDNA Removing Buffer: to ensure more reliable follow-up results, and simplify the design of qPCR primers, without the need to design primers across introns.
    * Different primers are selected for different downstream applications.

  • MB079: Recombinant Murine RNase Inhibitor (RI)

    * High purity;
    * Neither DNases nor RNases present;
    * Compatible with RT-PCR and RT-qPCR.

  • MB036-5G: 5G Reverse Transcriptase

    * Extensive template compatibility: compatible with various templates such as animals, plants, and viruses.
    * Super impurity tolerance: tolerant to common impurities (ethanol, isopropanol, water balance phenol, guanidine isothiocyanate, humic acid).
    * Higher reverse transcription efficiency than 4G reverse transcriptase.

     

    MB036-4G: 4G Reverse Transcriptase

    * Extensive template compatibility: compatible with various templates such as animals, plants, and viruses.
    * Super impurity resistance: resistant to common impurities (ethanol, isopropanol, water balance phenol, guanidine isothiocyanate, humic acid).
    * Long-fragment cDNA amplification (as long as 20 kb).

     

    MB036-MM: M-MLV(H-) Reverse Transcriptasee

    * Point mutations eliminate RNase H activity to obtain long cDNA products ≤5 kb.
    * Stable and reliable reverse transcription performance for RNA templates above 100 ng.
    * For 5’-RACE reaction and cDNA library construction.

  • MB049-EQ2G: 2x Rapid PCR Master Mix, with green dye

    * The PCR master mix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination and pipetting errors.
    * The tracking dye and precipitant have been added into the PreMix so that the PCR product can be directly loaded for electrophoresis.
    * Fast amplification speed: normally ~15 sec/kb; ~1 sec/kb if the amplification length is <1 kb.
    * High stability: repeated freezing and thawing for 50 times without significant decrease in activity.
    * Low cost: $400 for 50 ml 2x rapid PCR PreMix with dye. The cheapest PCR master mix for genotyping in the market.

  • MB067-EQ2G / MB067-EQ2B: 2x PCR PreMix, with dye (green, or blue)

    * The PCR master mix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination and pipetting errors.
    * The tracking dye and precipitant have been added into the PreMix so that the PCR product can be directly loaded for electrophoresis.

    Features of the Taq DNA polymerases contained in the PCR master mix:
    * High efficiency. The extension time of the Taq DNA polymerase is shorter than 30 seconds per kb DNA. Easily amplify DNA fragments up to 5 kb.
    * High purity. >98% homogeneous of the Taq DNA polymerase by SDS gel electrophoresis. No contamination detected in standard PCR test reactions.
    * Reproducible results. No visible activity change after storage of the Taq DNA polymerase at room temperature for 3 months to amplify a single-copy gene from human genome with high efficiency.
    * Terminal transferase activity of adding a single nucleotide (adenosine) at 3'-end of the extension product, facilitating TA cloning of PCR products.

  • MB040: GC rich PCR Enhancer

    The GC rich PCR enhancer can improve the efficiency of PCR amplification of GC rich DNA templates, increase the specificity of PCR products, and reduce non-specific and undesirable PCR products.

  • MB087: Hot Start Taq DNA Polymerase

    * High efficiency. The extension time of the Taq DNA polymerase is shorter than 30 seconds per kb DNA. Easily amplify DNA fragments up to 5 kb.
    * High specificity.
    * Reproducible results.
    * Terminal transferase activity of adding a single nucleotide (adenosine) at 3' end of the extension product, facilitating TA cloning of PCR products.
    * Low cost: $0.19+ per unit. The cheapest hot start Taq enzyme in the market.

     

    MB117: Hot Start Taq DNA Polymerase for Probe-based qPCR

    * High amplification specificity and sensitivity for detection of low-copy genes paired with the most suitable buffer optimized for qPCR and PCR.
    * For probe-based qPCR, excellent amplification curve within a wide quantitative range, and accurately quantify and detect target genes.
    * Compatibility in terms of template type, template GC content, and primer Tm values.
    * Good tolerance to impurities and is suitable for use in a variety of testing scenarios.
    * Low cost: $0.19+ per unit. The cheapest hot start Taq enzyme in the market.

     

  • MB118: Hot-Start High-Fidelity DNA Polymerase

    * Long fragment amplification: up to 40 kb from simple templates such as λDNA and plasmids, up to 20 kb from complex templates such as genomic DNA, and up to 10 kb from cDNA templates.
    * Fast amplification speed: normally ~30 sec/kb; ~0.5 sec/kb if the amplification length is <1 kb; 4-150 times that of conventional Taq DNA polymerase.
    * High specificity: Its amplification mismatch rate is 1/53 that of ordinary Taq polymerase and 1/6 that of Pfu polymerase.
    * Reproducible results: even with the GC-rich fragments and in the presence of PCR inhibitors.
    * Blunt-ended amplified products.
    * Low cost: $0.8 per unit. The cheapest hot start high fidelity Taq enzyme in the market.

  • MB016-HYT: High Yield Taq DNA Polymerase

    * Robust processivity. Up to 10 kb human genomic and 15 kb lamda DNA fragments have been tested for amplification.
    * High purity. >98% homogeneous of the Taq DNA polymerase by SDS gel electrophoresis. No contamination detected in standard PCR test reactions.
    * Reproducible results. No visible activity change after storage of the high yield Taq DNA polymerase at room temperature for 3 months to amplify a single-copy gene from human genome with high efficiency.
    * Proofreading with 5′ exonuclease activity.

  • MB040-HY2: 2x HY PCR Master Mix, with blue dye

    * The PCR master mix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination and pipetting errors.
    * The high yield PCR Master Mix has increased amplification robustness, fidelity, yield, and fragment length, as well as the ability to handle difficult or “dirty” templates.
    * The tracking dye and precipitant have been added into the PCR PreMix so that the PCR product can be directly loaded for electrophoresis.

    Features of the blend of Taq enzyme and a proofreading enzyme contained in the high yield PCR master mix:
    * Robust processivity. Up to 10 kb human genomic and 15 kb lamda DNA fragments have been tested for amplification.
    * High purity. >98% homogeneous of the Taq DNA polymerase by SDS gel electrophoresis. No contamination detected in standard PCR test reactions.
    * Reproducible results. No visible activity change after storage of the Taq DNA polymerase at room temperature for 3 months to amplify a single-copy gene from human genome with high efficiency.
    * Proofreading with 5′ exonuclease activity.

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