Our adenovirus production services for toxic genes include: 1) construction of an adenovirus vector for expression of a cytotoxic gene; 2) generation of adenoviruses carrying cytotoxic genes; 3) amplification and purification of adenoviruses carrying cytotoxic genes.
Our adenovirus production team has constructed, amplified, and purified thousands of adenoviruses for worldwide clients in more than a decade. An improved technology is used to simplify and accelerate construction of adenoviral vectors for researchers studying gene expression or gene silencing in mammalian systems.
Fast turnaround: 2 weeks.- Any gene;
- Any host cell, including primary cell;
- Highly efficient and precise.
Fast turnaround:
Knock-out cell line generation: 16-19 weeks.
Knock-in cell line generation: 20-24 weeks.Our Ad5-RGD and Ad5/35 adenovirus production services include: 1) construction of an Ad5-RGD and Ad5/35 adenovirus vector for expression or silencing of a gene; 2) generation of Ad5-RGD and Ad5/35 adenovirus; 3) amplification and purification of Ad5-RGD and Ad5/35 adenovirus.
Our adenovirus production team has constructed, amplified, and purified thousands of adenoviruses for worldwide clients in more than a decade. An improved technology is used to simplify and accelerate construction of adenoviral vectors for researchers studying gene expression or gene silencing in mammalian systems.
Fast turnaround: 2 weeks.Our retrovirus production services include: 1) construction of a retrovirus plasmid vector for expression or silencing of a gene; 2) retrovirus packaging; 3) retrovirus purification; 4) retrovirus titration.
We have always optimized our retrovirus production systems, including retrovirus plasmid construction and low endotoxin plasmid preparation, host cells for retrovirus packaging, cell culture medium and retrovirus packaging process, small and large scale retrovirus purification process.
Fast turnaround: 2-3 weeks.Our complete custom Adeno-associated Virus (AAV) production services include: 1) AAV plasmid vector construction; 2) AAV packaging; 3) AAV purification by iodixanol or column chromatography; 4) AAV titering by qPCR.
We have always optimized our AAV production systems, including AAV plasmid vector design and construction and low endotoxin plasmid preparation, host cells for AAV packaging, cell culture medium and AAV packaging process, small and large scale AAV purification process.
Fast turnaround: 2-3 weeks.Our complete custom lentivirus production services include: 1) lentivirus vector construction for expression or silencing of a gene; 2) lentivirus packaging; 3) lentivirus purification; 4) lentivirus titering.
We have always optimized our lentivirus production systems, including lentivirus plasmid construction and low endotoxin plasmid preparation, host cells for lentivirus packaging, cell culture medium and lentivirus packaging process, small and large scale lentivirus purification process.
Fast turnaround: 2-3 weeks.Experienced scientists working on antibody and cell line development services for decades. >90% success rate of stable cell line generation.
Stable cell lines developed with our proprietary recombinant antibody expression system with multiple selectable markers is very productive in expressing target proteins.
Fast turnaround:
Basic service: 7-8 weeks.
Premium service: 12-14 weeks.
Pool cells: 6 weeks.Experienced scientists working on antigen and antibody production, antibody engineering, lentivirus, and cell line development services for decades. >90% success rate of stable cell line generation.
Fast turnaround:
Antigen (peptide or protein) production: 3-6 weeks.
Mouse monoclonal antibody production: 20-24 weeks.
Screening phage or yeast libraries for fully human antibodies: 8-12 weeks.
Sequencing hybrioma antibody: 1-3 weeks.
Chimeric antibody production: 3-4 weeks.
Antibody humanization: 8-10 weeks.
Construction of CAR cassette, lentivirus packaging and production: 4-5 weeks.
T-cell activation, transduction, and CAR T-cell expansion: 4-5 weeks.
Confirmation of CAR expression, IFN-gamma ELISA, and cytotoxicity assay of transduced and expanded T-cells: 2 weeks.We provide large selection of pre-made adenovirus and complete custom adenovirus production services from small crude scale to large purified scale, including: 1) construction of an adenovirus vector for expression or silencing of a gene; 2) generation of adenovirus; 3) amplification and purification of adenovirus.
Our adenovirus production team has constructed, packaged, amplified, and purified thousands of adenoviruses for worldwide clients in more than a decade. An optimized technology is used to simplify and accelerate adenoviral vector construction for researchers studying gene expression or gene silencing in mammalian systems.
Fast turnaround: 2-3 weeks.- Any gene;
- Any host cell, including primary cell;
- Significantly improved efficiency of changing protein expression level compared to non-viral DNA transfection methods.
Fast turnaround:
Clonal stable cell line generation: 12-14 weeks.
Cell pool generation: 8-10 weeks.