Syd Labs has successfully developed human and monkey monoclonal antibodies using antigen specific single B cell-based antibody generation technologies. After detection and isolation of antigen specific memory B cells by flow cytometry-based single B cell sorting, the antibody variable regions (VH and VL) from single or clonal B cells are cloned and sequenced. Recombinant rabbit monoclonal antibodies are produced, purified, and tested for various applications, such as drug development and diagnostics.
Fast turnaround: 8-10 weeks once the polyclonal sera, PBMC, and antigen is available.BP001687-C011: Recombinant Human CD40 Ligand (CD40L) Protein
Source: E. coli-derived.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 IEU/ug).
BP000325-CYT-472: Recombinant Mouse Soluble CD-40 Ligand/TRAP
Source: E. coli-derived.
A non-glycosylated, Polypeptide chain containing 149 amino acids and having a molecular mass of 16409 Dalton.
The ED50 as determined by its ability to induce MIP-1 alpha & TNF-alpha from mouse splenocytes was found to be 0.1 ?/ml corresponding to a Specific Activity of 10 MIU/mg.
Purity > 98%, by SDS-PAGE and RP-HPLC.BP000325-CYT-670: Recombinant Human soluble CD40 Ligand/TRAP, His Tag
Source: E. coli-derived.
A single, non-glycosylated, polypeptide chain containing 169 amino acids (113-261 a.a.) and having a molecular mass of 18.3 kDa.
Purity > 95%, by SDS-PAGE.BP000326-CYT-245: Recombinant Human Soluble CD-40 Ligand/TRAP
Source: E. coli-derived.
A non-glycosylated, Polypeptide chain containing 149 amino acids and having a molecular mass of 16308 Dalton.
The ED50 as determined by the dose-dependant stimulation of IL-12 induction & IL-8 production by human PBMC was found to be 5-10 ug/ml.
Purity > 97%, by SDS-PAGE and RP-HPLC.