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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Conditions of optimal HSP60 antibody performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Background
Heat shock 60KD protein (HSP60) is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNFsecretion by CD4-positive and CD8-positive T cells. HSP60 enhances Treg activity via TLR2, leading to activation of an intracellular signaling cascade that included p38, as well as inhibition of ERK phosphorylation. Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10, and it leads to downregulation of ERK, NFKB, and TBET expression. The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through TLR2 signaling.
PA000051-MA1049: HSP60 Monoclonal Antibody
Mouse Monoclonal Antibody.
Specificity/Sensitivity: Human, chicken, rat. No cross reactivity with other proteins.
Applications: Western blot: At 2-4?g/ml with the appropriate system to detect HSP60 in cells and tissues. Immunohistochemistry(P): At 4-8?g/ml to detect HSP60 in formalin fixed and paraffin embedded tissues.
Reactivity: Human, Rat, Chicken.
Immunogen: Recombinant human heat shock protein 60 (HSP60)1
Purification: Purified by the goat anti-mouse IgG affinity chromatography.
Form of Antibody: Lyophilized from 1.2% sodium acetate, with 2 mg BSA and 0.01 mg NaN3 as preservative.
Clone: SJ-60.
Isotype: Mouse IgG1.
Storage/Stability: Stable for 1 year at -20°C and 3 months at 4°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.
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