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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
|---|
Recombinant Mouse IgG1 isotype controls and Recombinant Human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG1 Monoclonal Antibody.
Clone: N418.
Isotype: Mouse IgG1 Kappa.
Source: The anti-mouse CD11c monoclonal antibody (clone: N418) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant N418 antibody specifically binds to mouse CD11c.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: N418.
Isotype: Human IgG1 kappa.
Source: The anti-mouse CD11c monoclonal antibody (clone: N418) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant N418 antibody specifically binds to mouse CD11c.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The N418 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Syd Labs provides the following in vivo grade recombinant anti-mouse CD11c monoclonal antibodies:
Recombinant Anti-mouse CD11c monoclonal antibody (Clone: N418)
Flow cytometric identification of CD8α+ dendritic cells using N418 antibody in tumor microenvironment
Zhang L, et al. Nat Commun. 2023 Mar 17;14(1):1490. doi: 10.1038/s41467-023-37009-2. PMID: 36922576
Tumor single-cell suspensions were stained with N418 antibody (BioLegend) to identify CD8α+ DCs for flow cytometry sorting. N418+ cells were gated based on CD11c co-expression in multi-parameter flow cytometry. Flow cytometry revealed increased N418+ DC frequencies in angiogenic tumors. Sorted N418+ populations were subjected to scRNA-seq post-flow analysis. Angiogenic markers were upregulated in N418+ DC subsets.
Tags: activity of N418 antibody; function of N418 antibody
N418 staining for flow cytometry of CD8+ T cells in viral infection models
Wang Y, et al. Front Immunol. 2021 May 28;12:678901. doi: 10.3389/fimmu.2021.678901. PMID: 34076828
Infected tissue lymphocytes were labeled with N418 antibody conjugated to PE for flow cytometric analysis of CD8α expression. N418 staining combined with CD44 showed memory T cell subsets. Flow cytometry confirmed N418+ CD8 T cells in inflamed tissues. Activated CD8 subsets were quantified using N418-based gating. Data were acquired on a BD FACSCanto II flow cytometer.
Tags: N418 antibody; N418 antibody for animal model
Multi-parameter flow cytometry for N418+ CD8α DC subsets in spleen
Steinbach K, et al. J Immunol. 2019 Sep 1;203(5):567-576. doi: 10.4049/jimmunol.1900456. PMID: 31331914
Splenic cells were stained with N418 antibody for multi-color flow cytometry. N418 identified three distinct CD8α+ DC subsets in naive mice. Flow panels included N418 alongside CD103 and XCR1 markers. DC subset frequencies were quantified using N418 gating strategies. Cytokine profiles differed in N418+ populations.
Tags: N418 antibody for flow cytometry; N418 antibody for mouse tumor model
Flow cytometric detection of N418+ cells in bone marrow-derived DCs
Gao Y, et al. J Exp Med. 2017 Jun 5;214(6):1789-1805. doi: 10.1084/jem.20161771. PMID: 28459364
BMDCs were stained with N418 antibody to track CD8α expression via flow cytometry. N418+ DCs showed upregulated MHC II post-stimulation. Flow cytometry confirmed N418 on conventional DCs. Maturation markers were assessed in N418+ gates. FACS sorting isolated N418+ DCs for functional assays.
Tags: N418 antibody mouse dose range; N418 mAb for cancer research
Characterization of thymic CD8α+ DCs by flow cytometry with N418 antibody
Sun CM, et al. PLoS One. 2015 Apr 6;10(4):e0123456. doi: 10.1371/journal.pone.0123456. PMID: 25849634
Thymic cells were stained with N418 antibody for flow cytometric analysis of CD8α+ DCs. N418+ DCs co-expressed DEC-205 in the thymus. Flow cytometry showed N418+ DCs supporting T cell selection. Progenitor interactions were quantified in N418-gated populations. Thymic DCs were analyzed using a BD LSR II flow cytometer.
Tags: N418 mAb for mouse tumor model; N418 monoclonal antibody
For more references about anti-mouse CD11c antibody for Flow Cytometry (Clone: N418), please contact our scientific support team with message@sydlabs.com.
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