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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant Mouse IgG1 isotype controls and Recombinant Human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG1 Monoclonal Antibody.
Clone: N418.
Isotype: Mouse IgG1 Kappa.
Source: The anti-mouse CD11c monoclonal antibody (clone: N418) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant N418 antibody specifically binds to mouse CD11c.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: N418.
Isotype: Human IgG1 kappa.
Source: The anti-mouse CD11c monoclonal antibody (clone: N418) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant N418 antibody specifically binds to mouse CD11c.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
Clone: N418.
Isotype: Human IgG1 Fc Silent Kappa.
Source: The site-directed biotinylated anti-mouse CD11c monoclonal antibody (clone: N418) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant N418 antibody specifically binds to mouse CD11c.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The N418 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
1、Rapid monoclonal antibody generation via dendritic cell targeting in vivo
Licea, et al. Hybrid Hybridomics. 2003 Feb;22(1):23-31. doi: 10.1089/153685903321538053. PMID: 12713687
To address this question we immunized mice with IgG-peptide conjugates prepared with the hamster anti-murine CD11c MAb N418. ...Synthetic peptides corresponding to two different exposed regions of DC-specific ICAM-3 grabbing nonintegrin (DC-SIGN), a human C-type lectin, were conjugated to N418 using thiol-based chemistry. ...The N418 MAb served as the targeting molecule and synthetic peptides as the Ag (MAb-Ag). ...A rapid and peptide specific serum IgG response was produced by Day 7 when the synthetic peptides were linked to the N418 MAb, compared to peptide co-delivered with the N418 without linkage. ...To address this question we immunized mice with IgG-peptide conjugates prepared with the hamster anti-murine CD11c MAb N418.
Tags: activity of N418 antibody; function of N418 antibody
2、Rapid antibody responses by low-dose, single-step, dendritic cell-targeted immunization
Wang, H., et al. Proc Natl Acad Sci U S A. 2000 Jan 18;97(2):847-52. doi: 10.1073/pnas.97.2.847. PMID: 10639168
Although the exact cellular role of CD11c is unclear, binding of mAb N418 has been demonstrated not to interfere with the antigen presentation process of dendritic cells (15) and has been reported to induce an antibody response to itself after a single inoculation. ...Thus, mAb N418 is an excellent candidate for antigen delivery. ...The reporter protein antigen we chose is a polyclonal goat anti-hamster IgG antibody, which binds well to mAb N418 and is a foreign protein to the mouse. ...To determine whether antibody responses can be enhanced by targeting dendritic cells with the hamster mAb N418, immunizations were performed with goat anti-hamster IgG antibody as the model antigen. ...The specificity and potential efficacy of this approach were evaluated by comparing separate groups of mice immunized with either a mixture of mAb N418 and goat anti-hamster IgG antibody or a control immunogen.
Tags: N418 mAb for mouse tumor model; N418 monoclonal antibody
For more references about anti-mouse CD11c antibody for flow cytometry (Clone: N418) please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following in vivo grade recombinant anti-mouse CD11a/CD11b/CD11c monoclonal antibodies:
Recombinant Anti-mouse CD11c monoclonal antibody (Clone: N418)
Recombinant Anti-mouse CD11b monoclonal antibody (Clone: M1/70.15.11.5.HL)
Recombinant Anti-mouse CD11b monoclonal antibody (Clone: 5C6)
Recombinant Anti-mouse CD11a (LFA-1α) monoclonal antibody (Clone: M17/4.4.11.9)
Recombinant Anti-mouse CD11a (LFA-1α) monoclonal antibody (Clone: FD441.8)
Syd Labs provides the following recombinant anti-mouse CD11a/CD11b/CD11c monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD11c monoclonal antibody (Clone: N418) for flow cytometry
Syd Labs provides the following in vivo grade recombinant anti-human CD11a/CD11b/CD11c monoclonal antibodies:
Recombinant Anti-human CD11a (LFA-1α) monoclonal antibody (Clone: TS1/22.1.1.13)
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