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Recombinant rat IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Rat IgG2b Monoclonal Antibody.
Clone: FD441.8.
Isotype: Rat IgG2b Kappa.
Source: The anti-mouse CD11a (LFA-1 alpha) monoclonal antibody (clone: FD441.8) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: FD441.8) specifically binds to mouse CD11a (LFA-1 alpha).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD11a (LFA-1 alpha) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD11a (LFA-1 alpha) monoclonal antibody of clone FD441.8 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Pancreatic islet xenograft survival in mice is extended by a combination of alpha-1-antitrypsin and single-dose anti-CD4/CD8 therapy.
Efrat Ashkenazi. , et al. PLoS One. 2013 May 22;8(5):e63625. doi: 10.1371/journal.pone.0063625. PMID: 23717456
Subtherapeutic co-stimulation blockade included an equal mixture of anti-LFA-1 and anti-CD154 monoclonal antibodies (MR-1 and FD441.8, respectively, BioXCell, West Lebanon, NH, USA), each at 25µl/injection at the concentration of 1.25 mg/ml, one day before transplantation and every three days thereafter. ...Explanted kidneys carrying implants were fixed in 10% formalin (Sigma) for 24 h and transferred into 70% ethanol. ...Rat pancreatic islets (50/well in 48-well plates in triplicate) were cultured with medium alone or with recombinant IL-1β (10 ng/ml, R&D Systems), in the presence or absence of a 1 h pretreatment with hAAT (0.5 mg/ml). ...In all experiments, the hAAT group is comprised of recipient mice that are transgenic for lung-derived hAAT. ...In order to achieve a robust immune response and improve detection of changes in DLN, as well as to encourage responses with low variability, skin xenotransplantation was performed.
Tags: anti-mouse CD11a (LFA-1α) FD441.8 antibody in mouse tumor model; bioactivity of anti-mouse CD11a (LFA-1α) FD441.8
Antibody-mediated B-cell depletion before adoptive immunotherapy with T cells expressing CD20-specific chimeric T-cell receptors facilitates eradication of leukemia in immunocompetent mice.
James, S. E., et al. Blood. 2009 Dec 24;114(27):5454-63. doi: 10.1182/blood-2009-08-232967. PMID: 19880489
The blocking anti–LFA-1 (lymphocyte function–associated antigen–1) antibody FD441.8 was obtained from Bio X Cell. ...In LFA-1–blocking experiments, T cells were labeled at 2 × 107 cells/mL in PBS 2.5mM EDTA containing 50 μg/mL FD441.8 at 4°C for 30 minutes, followed by washing twice in PBS. ...For analysis of T-cell/B-cell conjugates, T cells were labeled with 1μM DDAO-SE (Invitrogen) and B cells or BM185-mCD20 was labeled with 0.1μM CFSE (Invitrogen). ...The percentage of T cells in conjugates was calculated by dividing the percentage of CFSE+DDAO+ conjugates by the total percentage of DDAO+ events. ...Luciferase activity was analyzed in mice anesthetized with isoflurane 10 minutes after intraperitoneal injection of d-luciferin potassium salt (Caliper Life Sciences) at 150 mg/kg.
Tags: anti-CD11a (LFA-1α) clone FD441.8; FD441.8 anti-mouse CD11a (LFA-1α) antibody
For more references about Anti-mouse CD11a (LFA-1 alpha) Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.
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