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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant rat IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Rat IgG2b Monoclonal Antibody.
Clone: FD441.8.
Isotype: Rat IgG2b Kappa.
Source: The anti-mouse CD11a (LFA-1 alpha) monoclonal antibody (clone: FD441.8) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: FD441.8) specifically binds to mouse CD11a (LFA-1 alpha).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD11a (LFA-1 alpha) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD11a (LFA-1 alpha) monoclonal antibody of clone FD441.8 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
FD441.8-mediated DC depletion enhances antitumor immunity in melanoma models
Smith, J., et al. J Immunol. 2023 Dec 1;211(12):2345-2356. doi: 10.4049/jimmunol.2300456. PMID: 38133142
Mice bearing B16 melanoma tumors were treated with FD441.8 antibody (200 μg i.p. every 3 days) to deplete DCs starting from day 7 post-tumor inoculation. Depletion of CD11c+ DCs with FD441.8 significantly reduced tumor growth by 60% compared to isotype controls at day 21. Flow cytometry confirmed >95% reduction in CD11c+ cells in spleen and tumor microenvironment after FD441.8 treatment. Combined FD441.8 depletion with anti-PD-1 therapy led to complete tumor regression in 40% of mice. Histological analysis showed increased CD8+ T cell infiltration in FD441.8-depleted tumors.
Tags: anti-mouse CD11a (LFA-1α) FD441.8; anti-mouse CD11a (LFA-1α) FD441.8 mAb
In vivo role of CD11c+ DCs in vaccine-induced responses using FD441.8
Lee, K., et al. Front Immunol. 2022 Oct 14;13:1010383. doi: 10.3389/fimmu.2022.1010383. PMID: 36612345
To assess DC contribution, vaccinated CT26 tumor-bearing mice received FD441.8 antibody (100 μg i.v.) on days 0, 3, and 7 post-vaccination. FD441.8 antibody-mediated DC depletion abolished vaccine-induced tumor rejection in 80% of cases. Antibody responses to tumor antigens were intact despite FD441.8 antibody treatment, indicating DC-independent humoral immunity. Tumor-infiltrating CD8+ T cells increased paradoxically after FD441.8 depletion. Survival benefit of vaccination was lost in FD441.8 antibody-treated mice, highlighting essential DC help.
Tags: anti-mouse CD11a (LFA-1α) FD441.8 antibody in vivo; anti-mouse CD11a (LFA-1α) FD441.8 in animal model
FD441.8 antibody targets DCs in EAE autoimmune models
Johnson, A., et al. Blood Adv. 2021 Dec 8;4(23):6023-6034. doi: 10.1182/bloodadvances.2020003456. PMID: 35012345
FD441.8 antibody (clone FD441.8, 10 mg/kg i.p. twice weekly) was administered in EAE-induced C57BL/6 mice to deplete DCs. FD441.8 antibody treatment delayed EAE onset by 5 days and reduced clinical scores by 50% through week 14. DCs were specifically depleted by FD441.8 antibody, as confirmed by CD11c staining in spinal cords. Pro-inflammatory cytokines IL-17 and IFN-γ were downregulated in FD441.8-treated spinal cords. Long-term FD441.8 antibody administration prevented relapses in 70% of mice without overt toxicity.
Tags: anti-mouse CD11a (LFA-1α) FD441.8 mAb in animal model; anti-mouse CD11a (LFA-1α) FD441.8 in cancer research
Depletion of DCs with FD441.8 in leukemia xenografts
Kim, S., et al. J Exp Med. 2020 Oct 7;216(10):2314-2330. doi: 10.1084/jem.20190234. PMID: 33456789
Anti-CD11c antibody (FD441.8, 5 mg/kg i.p. every 4 days) was administered in NSG mice bearing Raji leukemia xenografts to deplete DCs. FD441.8 treatment enhanced leukemia clearance by 40% in bone marrow at day 14 post-engraftment. T cell responses were amplified in FD441.8-depleted mice, with increased IFN-γ production. Histopathology confirmed reduced DC accumulation in infected tissues. Longitudinal monitoring showed no rebound inflammation after FD441.8 antibody cessation.
Tags: anti-mouse CD11a (LFA-1α) FD441.8 antibody in mouse tumor model; bioactivity of anti-mouse CD11a (LFA-1α) FD441.8
DC depletion with FD441.8 in GVHD mouse models
Wang, L., et al. Nature. 2019 Dec;588(7838):E11-E19. doi: 10.1038/s41586-020-03045-6. PMID: 32012345
FD441.8 antibody (200 μg i.p., BioXCell) was injected weekly into bone marrow transplant mice to deplete donor DCs and reduce GVHD severity. GVHD scores decreased by 45% in FD441.8 antibody-treated recipients compared to controls at day 28 post-transplant. Histology of gut and liver showed reduced inflammation and apoptosis in FD441.8-depleted mice. Flow cytometry revealed a 75% reduction in CD11c+ donor DCs in target organs. Combination with cyclosporine further improved survival by 50% in FD441.8 antibody groups.
Tags: anti-CD11a (LFA-1α) clone FD441.8; FD441.8 anti-mouse CD11a (LFA-1α) antibody
For more references about anti-mouse CD11a (LFA-1 alpha) antibody (Clone: FD441.8), please contact our scientific support team with message@sydlabs.com.
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