PA007611.r2b: In Vivo Grade Recombinant Anti-mouse CD11b Monoclonal Antibody (Clone: 5C6), Rat IgG2b Kappa
Recombinant Rat IgG2b Monoclonal Antibody.
Clone: 5C6.
Isotype: Rat IgG2b Kappa.
Source: The anti-mouse CD11b monoclonal antibody (clone: 5C6) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: 5C6) specifically binds to mouse CD11b.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD11b protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD11b monoclonal antibody of clone 5C6 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
References of anti-mouse CD11b antibody (Clone: 5C6):
Microglial CD11b Knockout Contributes to Axonal Debris Clearance and Axonal Degradation Attenuation via IGF-1 After Acute Optic Nerve Injury
Li, Y., et al. Invest Ophthalmol Vis Sci. 2023 Apr 3;64(4):6. doi: 10.1167/iovs.64.4.6. PMID: 37096094
The primary antibodies used in this work are anti-Iba1(Novus, NB100-1028), anti-CD11b (Bio-Rad, Hercules, CA, USA; MCA711), anti-CD68 (Bio-Rad, MCA1957), CD206 (Cell Signaling Technology, Danvers, MA, USA; 24595), anti–IGF-1 (Abcam, ab9572), anti-Arginase 1 (ABclonal Science, Inc, Woburn, MA, USA; A4923), and anti-16/32 (Novis Biologicals, Centennial, CO, USA, NBP2-52644). To investigate the role of CD11b in optic nerve injury, we first examined the protein expression levels of CD11b around the crush site in a mouse ONC model. The WB results (Figs. 1A and B) suggest CD11b was significantly elevated on day 5 after injury, peaked on day 7, and remained at a high level on day 14. Western blot and immunofluorescence were used to detect CD11b expression in the mouse optic nerve crush (ONC) model. Bioinformatics analysis predicted the possible role of CD11b.
Tags: anti-mouse CD11b 5C6; anti-mouse CD11b 5C6 antibody
Impact of S1P Mimetics on Mesenteric Ischemia/Reperfusion Injury
Potì, F., et al. Pharmaceuticals (Basel). 2020 Oct 9;13(10):298. doi: 10.3390/ph13100298. PMID: 33050161
Rat anti Mouse CD11b antibody, clone 5C6 (MCA711) used to demonstrate microglia in mouse brain by immunohistochemistry on cryostat sections. Mesenteric ischemia/reperfusion (I/R) injury is a life-threatening condition associated with high morbidity and mortality. Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid whose plasma levels are increased during I/R. We evaluated the effect of S1P on human microvascular endothelial cells (HMEC-1) and the therapeutic potential of the S1P1 receptor agonist, fingolimod (FTY720), in a mouse model of mesenteric I/R. FTY720 reduced I/R-induced intestinal injury, neutrophil infiltration and oxidative stress.
Tags: anti-mouse CD11b 5C6 in vivo; anti-mouse CD11b 5C6 antibody in vivo
Decreased atherosclerosis in CX3CR1-/- mice reveals a role for fractalkine in atherogenesis
Lesnik, P., et al. J Clin Invest. 2003 Feb;111(3):333-40. doi: 10.1172/JCI15589. PMID: 12569158
Tissue sections were incubated with primary antibodies against Mac-1 (CD11b, clone 5C6, Serotec), followed by biotinylated rabbit anti-rat IgG. Fractalkine is a chemokine expressed on activated endothelial cells that mediates leukocyte adhesion and migration via the CX3CR1 receptor. We examined the role of fractalkine in atherosclerosis by studying CX3CR1-deficient (CX3CR1-/-) mice. CX3CR1-/- mice showed a 60% decrease in atherosclerotic lesion size compared with wild-type mice. The reduction in lesion size was accompanied by a decrease in Mac-1-positive monocytes in the lesions.
Tags: anti-mouse CD11b 5C6 in animal model; anti-mouse CD11b 5C6 antibody in animal model
Locally up-regulated lymphotoxin alpha, not systemic tumor necrosis factor alpha, is the principle mediator of murine cerebral malaria
Engwerda, C. R., et al. J Exp Med. 2002 May 20;195(10):1263-9. doi: 10.1084/jem.20020683. PMID: 12021316
Sections were stained with anti-CD11b (clone 5C6, Serotec) or anti-F4/80 (clone F4/80, Serotec) to identify macrophages. Cerebral malaria is a serious complication of Plasmodium falciparum infection. We have examined the roles of TNF-alpha and lymphotoxin alpha (LT alpha) in a murine model of cerebral malaria. LT alpha-/- mice were completely resistant to cerebral malaria despite high levels of systemic TNF-alpha. Local up-regulation of LT alpha in the brain was responsible for cerebral malaria pathogenesis.
Tags: anti-mouse CD11b 5C6 antibody in cancer research; anti-mouse CD11b 5C6 in mouse tumor model
Monoclonal antibody to the murine type 3 complement receptor inhibits adhesion of myelomonocytic cells in vitro and inflammatory cell recruitment in vivo and can thereby potentiate infection
Rosen, H., et al. J Exp Med. 1987 Dec 1;166(6):1685-701. doi: 10.1084/jem.166.6.1685. PMID: 2445894
Monoclonal antibody 5C6, which recognizes the murine type 3 complement receptor (CR3), was used to inhibit myelomonocytic cell adhesion in vitro and inflammatory cell recruitment in vivo. The murine type 3 complement receptor (CR3) mediates both adhesion of myelomonocytic cells to endothelium and phagocytosis of C3bi-coated particles. In vivo, 5C6 mAb treatment inhibited inflammatory cell recruitment in a thioglycollate-induced peritonitis model. This inhibition led to potentiation of Listeria monocytogenes infection by preventing focusing of phagocytes at infection sites. These findings demonstrate the critical role of CR3 in inflammatory responses and host defense.
Tags: anti-mouse CD11b antibody 5C6; clone 5C6 of anti-mouse CD11b antibody
For more references about anti-mouse CD11b antibody (Clone: 5C6), please contact our scientific support team with message@sydlabs.com.