Recombinant Rat IgG2b Monoclonal Antibody.
Clone: 5C6.
Isotype: Rat IgG2b Kappa.
Source: The anti-mouse CD11b monoclonal antibody (clone: 5C6) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: 5C6) specifically binds to mouse CD11b.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD11b protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD11b monoclonal antibody of clone 5C6 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Odobasic, D., et al.
Blood. 2013 May 16;121(20):4195-204. doi: 10.1182/blood-2012-09-456483.
PMID: 23509155
Murine neutrophils were identified as CD11bhiCD11c−MHC-IIneg side-scatterhi cells that were Ly-6G+ or Gr-1hi using anti-CD11b/APC-Cy7 (BD biosciences), anti-CD11c/PE, anti-MHC-II/APC, anti-Ly-6G/PE (1A8; BD biosciences), and anti-Gr-1/Alexa647 (RB6-8C5; DNAX). ...6G+CD11bhiCD11c−MHC-IIneg side-scatterhi cells; flow cytometry) in draining LNs of normal (n = 6) and subcutaneously OVA/LPS-injected mice (n = 6/time point). ...Although it is possible that some of these cells are immature granulocytic myeloid-derived suppressor cells (because they are also CD11b+Ly-6G+)29 which may, therefore, contribute to MPO-mediated effects on DCs, they displayed standard features of mature neutrophils, including high Ly-6G expression and granularity (side-scatterhi). ...To examine whether DC Mac-1 is involved in MPO-mediated regulation of DC activation, we tested the effect of MPO on DCs in the presence or absence of anti-Mac-1 antibody (5C6). ...5C6 blocks leukocyte adhesion via Mac-121 but affects DC activation in a similar manner as anti-Mac-1 (M1/70), which ligates/activates DC Mac-125 (supplemental Figure 5).
(Tags: anti-mouse CD11b 5C6; anti-mouse CD11b 5C6 antibody)
Stokes, R. W., et al.
Infect Immun. 2004 Oct;72(10):5676-86. doi: 10.1128/IAI.72.10.5676-5686.2004.
PMID: 15385466
In some experiments, 10 μg of the monoclonal antibody 5C6 (ATCC; anti-murine CD11b) was added to the macrophages along with the first 250 μl of binding medium. ...The binding of syringed or sonicated M. tuberculosis to CD11b+/+ and CD11b−/− peritoneal macrophages in the absence of serum was assessed in five separate experiments. ...In an alternative approach, BALB/c mouse resident peritoneal macrophages were incubated with syringed or sonicated M. tuberculosis in the presence or absence of the CD11b-specific monoclonal antibody 5C6. ...As expected (46), 5C6 inhibited the binding of syringed M. tuberculosis to peritoneal macrophages (93.5% reduction in peritoneal macrophages binding more than 10 bacteria; P < 0.01) but had no effect on the increased binding of sonicated M. ...Following the addition of the bacteria, the different macrophage monolayers were incubated for 3 h, gently washed three times with binding medium at 4 or 37°C as appropriate, fixed for 10 min in 10% formaldehyde in ethanol, mounted cell side up on glass slides, stained with Kinyoun's carbol fuschin and malachite green, and enumerated as previously described.
(Tags: anti-mouse CD11b 5C6 in vivo; anti-mouse CD11b 5C6 antibody in vivo)
Henderson, R. B., et al.
Blood. 2003 Jul 1;102(1):328-35. doi: 10.1182/blood-2002-10-3228.
PMID: 12623845
To enter inflammatory sites, murine neutrophils use the 2 main β2 integrins, lymphocyte function-associated antigen-1 (LFA-1) (CD11a/CD18) and Mac-1 (CD11b/CD18) together with α4β1 integrin. ...The following rat mAbs were used in the in vivo experiments: PS2/3 (α4 integrin, CD49d; immunoglobulin G2b [IgG2b]),22 5C6 (Mac-1, CD11b; IgG2b),9 PyLT-1 (control, antipolyoma large T antigen; IgG2b), and YB238 (control, anti-Ras; IgG2a). ...In addition, the Gr-1int population expressed low levels of CD11c, DEC205, major histocompatibility complex (MHC) class II, CD40, B7-1, and B7-2 (data not shown) and expressed high levels of CD11b, making it unlikely that these cells represent a subset of dendritic cells or plasmacytoid dendritic cells, the latter of which are CD11b-. ...Significant inhibition of monocyte recruitment at 4 hours occurred with mAb PS2/3 (α4) but not with mAb 5C6 (Mac-1, CD11b) when compared with the PBS control. ...In a previous study using the same peritonitis model, mAb 5C6 contributed to the blocking of neutrophil recruitment,5 demonstrating that this Mac-1 mAb is a functional blocker within the context of this model.
(Tags: anti-mouse CD11b 5C6 antibody in cancer research; anti-mouse CD11b 5C6 in mouse tumor model)