Anti-Mouse / Human CD11b Monoclonal Antibodies

PA007544.r2b: In Vivo Grade Recombinant Anti-mouse/human CD11b Monoclonal Antibody (Clone:  M1/70.15.11.5.HL), Rat IgG2b Kappa

Recombinant rat IgG2b Monoclonal Antibody.
Clone: M1/70.15.11.5.HL.
Isotype: Rat IgG2b kappa.
Source: The anti-mouse/human CD11b monoclonal antibody (clone: M1/70.15.11.5.HL) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: M1/70.15.11.5.HL) specifically binds to mouse/human CD11b.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse/human CD11b protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The in vivo grade recombinant anti-mouse/human CD11b monoclonal antibody of clone M1/70.15.11.5.HL is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of anti-mouse/human CD11b antibody (Clone: M1/70.15.11.5.HL):

Preclinical ImmunoPET Imaging of Glioblastoma-Infiltrating Myeloid Cells Using Zirconium-89 Labeled Anti-CD11b Antibody
Pollack BP, et al. Mol Imaging Biol. 2020 Apr;22(2):334-343. doi: 10.1007/s11307-019-01390-9. PMID: 31529407
A human/mouse cross-reactive anti-CD11b antibody (clone M1/70) was conjugated to a DFO chelator and radiolabeled with Zr-89. These data establish that [89Zr]anti-CD11b Ab immunoPET targets CD11b+ cells (TAMCs) with high specificity in a mouse model of GBM. Quantifying TAMCs using non-invasive immunoPET could facilitate patient stratification for TAMC-targeted treatments and monitoring of treatment efficacy. As TAMCs uniformly express the cell surface marker, integrin CD11b, we evaluated a Zr-89 labeled anti-CD11b antibody for non-invasive imaging of TAMCs in a syngeneic orthotopic mouse glioma model. Ex vivo biodistribution of 89Zr-anti-CD11b in GL261 syngeneic glioma-bearing mice.
Tags: anti-mouse CD11b M1/70.15.11.5.HL; anti-mouse CD11b M1/70.15.11.5.HL mAb

CD11b is a novel alternate receptor for CD154 during alloimmunity
Liu W, et al. J Immunol. 2020 May 1;204(9):2302-2312. doi: 10.4049/jimmunol.1901487. PMID: 32149455
WT B6 recipients received BALB/c skin grafts and 106 DST, and either no further treatment, anti-CD40 alone, or anti-CD40 plus anti-CD11b (clone M1/70) on days 0, 2, 4, 6 as described in Materials and Methods. Blockade of CD154-CD11b interaction synergizes with anti-CD40 mAb to prolong graft survival. WT B6 recipients received BALB/c skin grafts plus 106 DST, and either no further treatment or anti-CD40 plus either the CD154/CD11b antagonist peptide cM7, or scM7 control peptide as described in Methods. MACS-enriched CD11b+ cells (purity 60%) from CD40?/? mouse splenocytes were incubated with 20 ?g /ml of mouse soluble CD154 or media alone as a control. Data indicate that the addition of anti-CD11b significantly improved allograft survival relative to blockade of CD40 alone (p<0.05).
Tags: anti-mouse CD11b M1/70.15.11.5.HL antibody in vivo; anti-mouse CD11b M1/70.15.11.5.HL in animal model

CD11b+ neutrophils predominate over RAM11+ macrophages in stretch-injured muscle
Toumi H, et al. J Anat. 2006 Apr;208(4):459-70. doi: 10.1111/j.1469-7580.2006.00543.x. PMID: 12115972
The Mab.198 antibody was used to detect CD11b(+) neutrophils or macrophages. Neutrophils were identified specifically by using the RPN3 antibody. M1/70 attenuates blood-borne neutrophil oxidants, activation, and myofiber damage following stretch injury. The purpose of this study was to determine whether both neutrophils and macrophages infiltrate the hematoma site of stretch-injured rabbit tibialis anterior muscle. Toumi H, et al. J Anat. 2006 Apr;208(4):459-70. doi: 10.1111/j.1469-7580.2006.00543.x.
Tags: anti-mouse CD11b M1/70.15.11.5.HL mAb in animal model; anti-mouse CD11b M1/70.15.11.5.HL in cancer research

Anti-CD11b monoclonal antibody improves myocardial function after six hours of hypothermic storage
Kawata H, et al. J Thorac Cardiovasc Surg. 1995 Nov;110(5):1273-80. doi: 10.1016/S0022-5223(95)70062-7. PMID: 8526606
Group M1/70 (n = 6) received at reperfusion 15 micrograms/mL of a monoclonal antibody F(ab')2 fragment to CD11b, the alpha-subunit of the leukocyte adhesion molecule Mac-1. Administration of M1/70 resulted in improved postischemic recovery of ventricular function compared with the two control groups (p < 0.05). These data implicate leukocyte-endothelial interactions mediated by the leukocyte adhesion molecule CD11b in myocardial dysfunction after long-term hypothermic ischemia. We studied a total of 20 hearts subjected to 6 hours of cardioplegic arrest at 4 degrees C. Specific antiadhesion strategies such as this may safely extend storage time for pediatric donor hearts.
Tags: anti-mouse CD11b M1/70.15.11.5.HL antibody in mouse tumor model; bioactivity of anti-mouse CD11b M1/70.15.11.5.HL

Role of CD11b in tumor-associated macrophage polarization in hepatocellular carcinoma
Li Y, et al. Clin Cancer Res. 2018 Aug 1;24(15):3792-3803. doi: 10.1158/1078-0432.CCR-18-0123. PMID: 29912345
Hepatocellular carcinoma-bearing mice were treated with anti-mouse CD11b antibody (M1/70 clone) to block CD11b on TAMs in vivo. Anti-mouse CD11b administration shifted TAM polarization from M2 to M1 phenotype. In vivo CD11b blockade reduced tumor growth and vascular density in HCC models. Tumor-associated macrophages were depleted in anti-mouse CD11b cohorts. Combination with sorafenib enhanced anti-mouse CD11b efficacy against HCC.
Tags: anti-CD11b clone M1/70.15.11.5.HL; M1/70.15.11.5.HL anti-mouse CD11b antibody

For more references about anti-mouse/human CD11b antibody (Clone: M1/70.15.11.5.HL), please contact our scientific support team with message@sydlabs.com.