Name: Anti-Human CD19 Monoclonal Antibodies
Brand: Syd Labs
SKU: PA007272.m2a
Price: 200.00 USD
Availability: InStock
Rating: 5 (10 reviews)

Catalog No.

Product Name

Size

List Price (US$)

Quantity

PA007272.m2a

In vivo Grade Recombinant Anti-human CD19 Monoclonal Antibody (Clone: B43), Mouse IgG2a Kappa

1 mg

200.00

PA007272.m2a

In vivo Grade Recombinant Anti-human CD19 Monoclonal Antibody (Clone: B43), Mouse IgG2a Kappa

5 mg

500.00

PA007272.m2a

In vivo Grade Recombinant Anti-human CD19 Monoclonal Antibody (Clone: B43), Mouse IgG2a Kappa

25 mg

1250.00

Description

PA007272.m2a: In vivo Grade Recombinant Anti-human CD19 Monoclonal Antibody, Mouse IgG2a Kappa (Clone: B43)

Recombinant mouse IgG2a Monoclonal Antibody.
Clone: B43.
Isotype: Mouse IgG2a kappa.
Source: The anti-human CD19 monoclonal antibody (clone: B43) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: B43) specifically binds to the human CD19 protein.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD19 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

Background

The B43 antibody binds to the human CD19 protein, a transmembrane protein expressed in all B lineage cells including human plasma cells. CD19 plays two major roles in human B cells: it acts as an adaptor protein to recruit cytoplasmic signaling proteins to the membrane, and works within the CD19/CD21 complex to decrease the threshold for B cell receptor signaling pathways. CD19 is a biomarker for B lymphocyte development, lymphoma diagnosis and can be utilized as a target for leukemia immunotherapies.

References of anti-human CD19 antibody (B43):

Anti-CD19 immunotoxin enhances the activity of chemotherapy in severe combined immunodeficient mice with human pre-B acute lymphoblastic leukemia
Herrera, L., et al. (2006). Leuk Lymphoma. 2006 Nov;47(11):2380-7. doi: 10.1080/10428190600822015. PMID: 17107913
The anti-CD19 immunotoxin B43-PAP, administered at 5 μg/mouse, was combined with chemotherapy in SCID mice with human pre-B acute lymphoblastic leukemia (ALL). The B43-PAP (anti-human CD19 mAb) targeted CD19+ cells in vivo, enhancing chemotherapy activity and improving survival in treated mice compared to controls.
Tags: anti-human CD19 B43; anti-human CD19 B43 mAb

Pharmacokinetics and biodistribution of radioimmunoconjugates of anti-CD19 antibody and single-chain Fv for treatment of human B-cell malignancy
Ghetie, V., et al. (1999). Cancer Immunol Immunother. 1999 May;48(2-3):111-9. doi: 10.1007/s002620050512. PMID: 10381531
The anti-CD19 B43 monoclonal antibody (anti-human CD19 mAb) and its derived single-chain Fv (FVS191) were evaluated in athymic nude mice bearing CD19-positive human lymphomas. The B43 mAb showed T1/2α and T1/2β phases of 0.72 h and 57 h, respectively, with peak uptake of 5.7% at 12 h, compared to 2.45% at 1 h for FVS191. The optimal radioimmunoconjugate uptake was observed at 6 h for B43, indicating its potential for radioimmunotherapy and immunotoxin therapy due to early tumor-specific uptake and favorable tissue-to-blood ratios.
Tags: anti-human CD19 B43 antibody in vivo; anti-human CD19 B43 in animal model

In vivo toxicity and pharmacokinetic features of B43 (anti-CD19)-genistein immunoconjugate in mice
Myers, D. E., et al. (1998). Leuk Lymphoma. 1998 Feb;28(3-4):329-40. doi: 10.3109/10428199809187032. PMID: 9516966
The B43-genistein immunoconjugate (anti-human CD19 mAb) was tested in vivo for toxicity and pharmacokinetics in monkeys, showing a plasma half-life of 10-23 h. The B43 antibody targeted genistein to CD19+ cells, but plasma samples from treated monkeys elicited anti-mouse IgG antibodies, indicating immunogenicity. Toxicity evaluations in mice confirmed the conjugate’s effects on CD19+ cells.
Tags: anti-human CD19 B43 mAb in animal model; anti-human CD19 B43 in cancer research

Immunotoxins for the treatment of B-cell lymphomas
Ghetie, M. A., et al. (1997). Mol Med. 1997 Jul;3(7):420-7. PMID: 9260154
Immunotoxins like B43-PAP (anti-human CD19 antibody) were shown to be effective for B-cell lymphomas in vivo. The B43 antibody was conjugated to toxins for treatment, demonstrating efficacy in targeting CD19+ cells. The study discusses clinical trials of B43-PAP, highlighting its potential for B-cell malignancy therapy.
Tags: anti-human CD19 B43 mAb in cancer research; anti-human CD19 B43 mAb in human tumor model

Membrane-associated CD19-LYN complex is an endogenous p53-independent and Bcl-2-independent regulator of apoptosis in human B-lineage lymphoma cells
Myers, D. E., et al. (1995). Proc Natl Acad Sci U S A. 1995 Oct 10;92(21):9575-9. doi: 10.1073/pnas.92.21.9575. PMID: 7568175
The B43 monoclonal antibody (anti-human CD19 mAb) was used to cross-link membrane-associated CD19, activating the LYN kinase signaling pathway and inducing apoptosis in B-lineage lymphoma cells in a p53- and Bcl-2-independent manner. The CD19-LYN complex was immunoprecipitated using B43 antibody, and flow cytometry confirmed high-level CD19 expression on lymphoma cells.
Tags: bioactivity of anti-human CD19 B43; anti-human CD19 B43 of low endotoxin

For more references about anti-human CD19 antibody (B43), please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following in vivo grade recombinant anti-mouse CD19 monoclonal antibodies:
Recombinant Anti-mouse CD19 monoclonal antibody (Clone: 1D3)

Syd Labs provides the following in vivo grade recombinant anti-human CD19 monoclonal antibodies:
Recombinant Anti-human CD19 monoclonal antibody (Clone: SJ25C1)
Recombinant Anti-human CD19 monoclonal antibody (Clone: B43)
Recombinant Anti-human CD19 monoclonal antibody (Clone: FMC63)

Syd Labs provides the following recombinant anti-mouse CD19 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD19 monoclonal antibody (Clone: 1D3) for flow cytometry

Syd Labs provides the following recombinant anti-human CD19 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD19 monoclonal antibody (Clone: SJ25C1) for flow cytometry
Recombinant Anti-human CD19 monoclonal antibody (Clone: FMC63) for flow cytometry
Recombinant Anti-human CD19 monoclonal antibody (Clone: B43) for flow cytometry

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Anti-Human CD19 Monoclonal Antibodies

B-lymphocyte antigen CD19, Cluster of Differentiation 19, B-Lymphocyte Surface Antigen B4, T-Cell Surface Antigen Leu-12, CVID3

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PA007272.m2a: In vivo Grade Recombinant Anti-human CD19 Monoclonal Antibody, Mouse IgG2a Kappa (Clone: B43)

References of anti-human CD19 antibody (B43):