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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant mouse IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant mouse IgG1 Monoclonal Antibody.
Clone: SJ25C1.
Isotype: Mouse IgG1 kappa.
Source: The anti-human CD19 monoclonal antibody (clone: SJ25C1) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: SJ25C1) specifically binds to the human CD19 protein.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD19 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Background
The SJ25C1 antibody binds to the human CD19 protein, a transmembrane protein expressed in all B lineage cells including human plasma cells. CD19 plays two major roles in human B cells: it acts as an adaptor protein to recruit cytoplasmic signaling proteins to the membrane, and works within the CD19/CD21 complex to decrease the threshold for B cell receptor signaling pathways. CD19 is a biomarker for B lymphocyte development, lymphoma diagnosis and can be utilized as a target for leukemia immunotherapies.
Safety and efficacy of a novel anti-CD19 chimeric antigen receptor T cell product targeting a membrane-proximal domain of CD19 with fast on- and off-rates against non-Hodgkin lymphoma: a first-in-human study
Zhang, Y., et al. (2023). Mol Cancer. 2023 Dec 9;22(1):200. doi: 10.1186/s12943-023-01886-9. PMID: 38066564
The SJ25C1 antibody (anti-human CD19) was used as a reference in flow cytometry to evaluate a novel anti-CD19 CAR targeting a membrane-proximal CD19 epitope. Compared to FMC63, the novel CAR showed improved efficacy against CD19 mutants (L174V, R163L) in lymphomas, with reduced activation-induced cell death and enhanced expansion, as assessed by BD FACSCanto II and FlowJo analysis.
Tags: anti-human CD19 SJ25C1; anti-human CD19 SJ25C1 mAb
Selective B cell depletion upon intravenous infusion of replication-incompetent anti-CD19 CAR lentivirus
Rive, C. M., et al. (2022). Mol Ther Methods Clin Dev. 2022 Sep 8;26:205-218. doi: 10.1016/j.omtm.2022.05.006. PMID: 35755944
The SJ25C1 antibody (anti-human CD19) identified CD19+ B cells in C57BL/6 mice after anti-CD19 CAR lentivirus infusion. Flow cytometry quantified selective B cell depletion in spleen and bone marrow, confirming no off-target effects, with SJ25C1 used to verify CD45R+ and CD19+ populations.
Tags: anti-human CD19 SJ25C1 in animal model; anti-human CD19 SJ25C1 antibody in animal model
Development and functional characterization of novel fully human anti-CD19 chimeric antigen receptors for T-cell therapy
Li, T., et al. (2021). J Cell Physiol. 2021 Aug;236(8):5838-5851. doi: 10.1002/jcp.30267. PMID: 33294237
The SJ25C1 antibody (anti-human CD19) served as a control in flow cytometry to compare binding affinity of novel humanized anti-CD19 CARs to FMC63. SJ25C1 confirmed comparable binding to CD19+ Raji cells, with flow cytometry and cytotoxicity assays validating CAR functionality for clinical use.
Tags: anti-human CD19 SJ25C1 in cancer research; anti-human CD19 SJ25C1 antibody in vivo
Efficacy and safety of CD19 CAR T constructed with a new anti-CD19 chimeric antigen receptor in relapsed or refractory acute lymphoblastic leukemia
Wang, Y., et al. (2020). J Hematol Oncol. 2020 Aug 17;13(1):111. doi: 10.1186/s13045-020-00953-8. PMID: 32799678
The SJ25C1 antibody (anti-human CD19) was used in flow cytometry to compare the binding affinity of a novel anti-CD19 CAR to FMC63. The CAR showed superior antitumor activity in xenograft models of B-ALL, with SJ25C1 confirming high CD19 expression on target cells and manageable toxicity in clinical settings.
Tags: anti-human CD19 SJ25C1 mAb in cancer research; anti-human CD19 SJ25C1 antibody
Fully human CD19-specific chimeric antigen receptors for T-cell therapy
Sommermeyer, D., et al. (2017). Leukemia. 2017 Oct;31(10):2191-2199. doi: 10.1038/leu.2017.57. PMID: 28202953
The SJ25C1 antibody (anti-human CD19) was used to develop humanized CD19-specific CARs, with its scFv compared to FMC63 in flow cytometry and cytotoxicity assays. SJ25C1-targeted epitopes overlapped with FMC63, and CAR-T cells showed comparable efficacy to murine-based CARs in redirecting T-cell function.
Tags: anti-human CD19 SJ25C1 antibody of low endotoxin; anti-human CD19 antibody SJ25C1
For more references about anti-human CD19 antibody (SJ25C1), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following in vivo grade recombinant anti-mouse CD19 monoclonal antibodies:
Recombinant Anti-mouse CD19 monoclonal antibody (Clone: 1D3)
Syd Labs provides the following in vivo grade recombinant anti-human CD19 monoclonal antibodies:
Recombinant Anti-human CD19 monoclonal antibody (Clone: SJ25C1)
Recombinant Anti-human CD19 monoclonal antibody (Clone: B43)
Recombinant Anti-human CD19 monoclonal antibody (Clone: FMC63)
Syd Labs provides the following recombinant anti-mouse CD19 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD19 monoclonal antibody (Clone: 1D3) for flow cytometry
Syd Labs provides the following recombinant anti-human CD19 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD19 monoclonal antibody (Clone: SJ25C1) for flow cytometry
Recombinant Anti-human CD19 monoclonal antibody (Clone: FMC63) for flow cytometry
Recombinant Anti-human CD19 monoclonal antibody (Clone: B43) for flow cytometry
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