My Cart [ 0 ]
Home > Antibodies > Anti-Human Antibodies (In Vivo) > Anti-Human CD19 Monoclonal Antibodies

Anti-Human CD19 Monoclonal Antibodies

B-lymphocyte antigen CD19, Cluster of Differentiation 19, B-Lymphocyte Surface Antigen B4, T-Cell Surface Antigen Leu-12, CVID3

Catalog No. Product Name Size List Price (US$) Quantity
PA007270.m1 In vivo Grade Recombinant Anti-human CD19 Monoclonal Antibody (Clone: SJ25C1), Mouse IgG1 Kappa 1 mg 150.00
PA007270.m1 In vivo Grade Recombinant Anti-human CD19 Monoclonal Antibody (Clone: SJ25C1), Mouse IgG1 Kappa 5 mg 350.00
PA007270.m1 In vivo Grade Recombinant Anti-human CD19 Monoclonal Antibody (Clone: SJ25C1), Mouse IgG1 Kappa 25 mg 900.00
Description

PA007270.m1: In vivo Grade Recombinant Anti-human CD19 Monoclonal Antibody, Mouse IgG1 Kappa (Clone: SJ25C1)

Recombinant mouse IgG1 Monoclonal Antibody. 
Clone: SJ25C1.
Isotype: Mouse IgG1 kappa.
Source: The anti-human CD19 monoclonal antibody (clone: SJ25C1) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: SJ25C1) specifically binds to the human CD19 protein.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD19 protein. 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

Background

The SJ25C1 antibody binds to the human CD19 protein, a transmembrane protein expressed in all B lineage cells including human plasma cells. CD19 plays two major roles in human B cells: it acts as an adaptor protein to recruit cytoplasmic signaling proteins to the membrane, and works within the CD19/CD21 complex to decrease the threshold for B cell receptor signaling pathways. CD19 is a biomarker for B lymphocyte development, lymphoma diagnosis and can be utilized as a target for leukemia immunotherapies.

References of anti-human CD19 antibody (SJ25C1):


Safety and efficacy of a novel anti-CD19 chimeric antigen receptor T cell product targeting a membrane-proximal domain of CD19 with fast on- and off-rates against non-Hodgkin lymphoma: a first-in-human study
Zhang, Y., et al. (2023). Mol Cancer. 2023 Dec 9;22(1):200. doi: 10.1186/s12943-023-01886-9. PMID: 38066564
The SJ25C1 antibody (anti-human CD19) was used as a reference in flow cytometry to evaluate a novel anti-CD19 CAR targeting a membrane-proximal CD19 epitope. Compared to FMC63, the novel CAR showed improved efficacy against CD19 mutants (L174V, R163L) in lymphomas, with reduced activation-induced cell death and enhanced expansion, as assessed by BD FACSCanto II and FlowJo analysis.
Tags: anti-human CD19 SJ25C1; anti-human CD19 SJ25C1 mAb

Selective B cell depletion upon intravenous infusion of replication-incompetent anti-CD19 CAR lentivirus
Rive, C. M., et al. (2022). Mol Ther Methods Clin Dev. 2022 Sep 8;26:205-218. doi: 10.1016/j.omtm.2022.05.006. PMID: 35755944
The SJ25C1 antibody (anti-human CD19) identified CD19+ B cells in C57BL/6 mice after anti-CD19 CAR lentivirus infusion. Flow cytometry quantified selective B cell depletion in spleen and bone marrow, confirming no off-target effects, with SJ25C1 used to verify CD45R+ and CD19+ populations.
Tags: anti-human CD19 SJ25C1 in animal model; anti-human CD19 SJ25C1 antibody in animal model

Development and functional characterization of novel fully human anti-CD19 chimeric antigen receptors for T-cell therapy
Li, T., et al. (2021). J Cell Physiol. 2021 Aug;236(8):5838-5851. doi: 10.1002/jcp.30267. PMID: 33294237
The SJ25C1 antibody (anti-human CD19) served as a control in flow cytometry to compare binding affinity of novel humanized anti-CD19 CARs to FMC63. SJ25C1 confirmed comparable binding to CD19+ Raji cells, with flow cytometry and cytotoxicity assays validating CAR functionality for clinical use.
Tags: anti-human CD19 SJ25C1 in cancer research; anti-human CD19 SJ25C1 antibody in vivo

Efficacy and safety of CD19 CAR T constructed with a new anti-CD19 chimeric antigen receptor in relapsed or refractory acute lymphoblastic leukemia
Wang, Y., et al. (2020). J Hematol Oncol. 2020 Aug 17;13(1):111. doi: 10.1186/s13045-020-00953-8. PMID: 32799678
The SJ25C1 antibody (anti-human CD19) was used in flow cytometry to compare the binding affinity of a novel anti-CD19 CAR to FMC63. The CAR showed superior antitumor activity in xenograft models of B-ALL, with SJ25C1 confirming high CD19 expression on target cells and manageable toxicity in clinical settings.
Tags: anti-human CD19 SJ25C1 mAb in cancer research; anti-human CD19 SJ25C1 antibody in human tumor model

Fully human CD19-specific chimeric antigen receptors for T-cell therapy
Sommermeyer, D., et al. (2017). Leukemia. 2017 Oct;31(10):2191-2199. doi: 10.1038/leu.2017.57. PMID: 28202953
The SJ25C1 antibody (anti-human CD19) was used to develop humanized CD19-specific CARs, with its scFv compared to FMC63 in flow cytometry and cytotoxicity assays. SJ25C1-targeted epitopes overlapped with FMC63, and CAR-T cells showed comparable efficacy to murine-based CARs in redirecting T-cell function.
Tags: anti-human CD19 SJ25C1 antibody of low endotoxin; anti-human CD19 antibody SJ25C1

For more references about anti-human CD19 antibody (SJ25C1), please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following in vivo grade recombinant anti-mouse CD19 monoclonal antibodies:
Recombinant Anti-mouse CD19 monoclonal antibody (Clone: 1D3)

Syd Labs provides the following in vivo grade recombinant anti-human CD19 monoclonal antibodies:
Recombinant Anti-human CD19 monoclonal antibody (Clone: SJ25C1)
Recombinant Anti-human CD19 monoclonal antibody (Clone: B43)
Recombinant Anti-human CD19 monoclonal antibody (Clone: FMC63)

Syd Labs provides the following recombinant anti-mouse CD19 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD19 monoclonal antibody (Clone: 1D3) for flow cytometry

Syd Labs provides the following recombinant anti-human CD19 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD19 monoclonal antibody (Clone: SJ25C1) for flow cytometry
Recombinant Anti-human CD19 monoclonal antibody (Clone: FMC63) for flow cytometry
Recombinant Anti-human CD19 monoclonal antibody (Clone: B43) for flow cytometry

Related Links

See our Privacy Policy