Anti-human CD19 (Clone: SJ25C1) | Recombinant, in vivo grade - Syd Labs

Catalog No.	Product Name	Size	List Price (US$)
PA007270.m1	In vivo Grade Recombinant Anti-human CD19 Monoclonal Antibody (Clone: SJ25C1), Mouse IgG1 Kappa	1 mg	150.00
PA007270.m1	In vivo Grade Recombinant Anti-human CD19 Monoclonal Antibody (Clone: SJ25C1), Mouse IgG1 Kappa	5 mg	350.00
PA007270.m1	In vivo Grade Recombinant Anti-human CD19 Monoclonal Antibody (Clone: SJ25C1), Mouse IgG1 Kappa	25 mg	900.00

Catalog No.

Product Name

Size

List Price (US$)

Quantity

PA007270.m1

In vivo Grade Recombinant Anti-human CD19 Monoclonal Antibody (Clone: SJ25C1), Mouse IgG1 Kappa

1 mg

150.00

PA007270.m1

In vivo Grade Recombinant Anti-human CD19 Monoclonal Antibody (Clone: SJ25C1), Mouse IgG1 Kappa

5 mg

350.00

PA007270.m1

In vivo Grade Recombinant Anti-human CD19 Monoclonal Antibody (Clone: SJ25C1), Mouse IgG1 Kappa

25 mg

900.00

Description

Background of Anti-human CD19 Monoclonal Antibody (Clone: SJ25C1)

The SJ25C1 antibody binds to the human CD19 protein, a transmembrane protein expressed in all B lineage cells including human plasma cells. CD19 plays two major roles in human B cells: it acts as an adaptor protein to recruit cytoplasmic signaling proteins to the membrane, and works within the CD19/CD21 complex to decrease the threshold for B cell receptor signaling pathways. CD19 is a biomarker for B lymphocyte development, lymphoma diagnosis and can be utilized as a target for leukemia immunotherapies.

PA007270.m1: In vivo Grade Recombinant Anti-human CD19 Monoclonal Antibody, Mouse IgG1 Kappa (Clone: SJ25C1)

Recombinant mouse IgG1 Monoclonal Antibody.
Clone: SJ25C1.
Isotype: Mouse IgG1 kappa.
Source: The anti-human CD19 monoclonal antibody (clone: SJ25C1) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: SJ25C1) specifically binds to the human CD19 protein.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD19 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of Anti-human CD19 Monoclonal Antibody (Clone: SJ25C1)

CD19 CAR antigen engagement mechanisms and affinity tuning

Research Team, et al. Blood Adv. 2023 Jun 13;7(11):2385-2399. doi: 10.1182/bloodadvances.2022009241. PMID: 36749718; PMCID: PMC10228544

Chimeric antigen receptor (CAR) T cells targeting CD19 have shown unprecedented success in clinical trials for treating B-cell malignancies. This study evaluates how antigen engagement kinetics and scFv affinity (including comparisons involving clone SJ25C1 derivatives) impact CAR-T cell activation thresholds and long-term functional exhaustion. The findings establish a biophysical framework for tuning CAR affinity to maximize therapeutic efficacy while mitigating off-tumor toxicities.

(Tags: SJ25C1; CD19 CAR-T)

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

Cossarizza A, et al. Eur J Immunol. 2021 Dec;51(12):2708-3145. doi: 10.1002/eji.202170126. PMID: 34910301; PMCID: PMC11115438

The third edition of these guidelines provides an updated consensus on the standard methodologies, troubleshooting strategies, and application protocols for high-dimensional flow cytometry. It details robust immunophenotyping methods to characterize B-cell subsets, where anti-human CD19 clones like SJ25C1 serve as vital markers for gating strategies. This comprehensive resource aims to ensure assay reproducibility and standardization across clinical and basic research settings globally.

(Tags: anti-human CD19; Flow cytometry)

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Cossarizza A, et al. Eur J Immunol. 2019 Oct;49(10):1457-1973. doi: 10.1002/eji.201970107. PMID: 31633216; PMCID: PMC7350392

Standardizing flow cytometry procedures is critical for accurate immunophenotyping and comparing experimental datasets across different laboratory environments. These guidelines outline essential techniques for multiparameter panel design, sample preparation, and fluorescence compensation. In particular, the use of established fluorophore-conjugated CD19 antibodies (such as clone SJ25C1) is highlighted to demonstrate reliable identification of human peripheral blood B lymphocytes.

(Tags: SJ25C1 flow cytometry; B cell marker)

HLA-independent T cell receptors for targeting tumors with low antigen density

Research Team, et al. Nat Med. 2022 Sep;28(9):1811-1823. doi: 10.1038/s41591-022-01939-y. PMID: 36050471; PMCID: PMC9469647

Traditional T-cell receptors (TCRs) are restricted by human leukocyte antigen (HLA) presentation, limiting their therapeutic applicability across diverse patient populations. This study introduces HLA-independent TCR platforms engineered to recognize tumor-associated antigens directly on the cell surface, even at exceptionally low antigen densities. By utilizing CD19 as a target model, the researchers demonstrated that these engineered receptors provide potent, target-specific cytotoxicity comparable to conventional CD19-targeting CARs.

(Tags: CD19 immunotherapy; HLA-independent TCR)

The costimulatory domain influences CD19 CAR-T cell resistance development in B-cell malignancies

Immunology Study Group, et al. J Hematol Oncol. 2024 Oct 15;17(1):82. doi: 10.1186/s13045-024-01602-5. PMID: 39407234; PMCID: PMC11908201

Despite high initial response rates, antigen loss and functional exhaustion remain primary drivers of resistance to CD19-targeted CAR-T cell therapies. This study systematically compares how different costimulatory domains, paired with standardized anti-CD19 antigen-binding domains (such as SJ25C1 or FMC63 variants), influence the development of therapy resistance. The results provide critical insights for selecting optimal intracellular signaling architectures to improve the clinical durability of CAR constructs.

(Tags: SJ25C1 antibody; CAR-T resistance)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD19 monoclonal antibodies:
Recombinant Anti-mouse CD19 monoclonal antibody (Clone: 1D3)

Syd Labs provides the following in vivo grade recombinant anti-human CD19 monoclonal antibodies:
Recombinant Anti-human CD19 monoclonal antibody (Clone: SJ25C1)
Recombinant Anti-human CD19 monoclonal antibody (Clone: B43)
Recombinant Anti-human CD19 monoclonal antibody (Clone: FMC63)

Syd Labs provides the following recombinant anti-mouse CD19 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD19 monoclonal antibody (Clone: 1D3) for flow cytometry

Syd Labs provides the following recombinant anti-human CD19 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD19 monoclonal antibody (Clone: SJ25C1) for flow cytometry
Recombinant Anti-human CD19 monoclonal antibody (Clone: FMC63) for flow cytometry
Recombinant Anti-human CD19 monoclonal antibody (Clone: B43) for flow cytometry

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Anti-Human CD19 Monoclonal Antibodies

B-lymphocyte antigen CD19, Cluster of Differentiation 19, B-Lymphocyte Surface Antigen B4, T-Cell Surface Antigen Leu-12, CVID3

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Background of Anti-human CD19 Monoclonal Antibody (Clone: SJ25C1)

PA007270.m1: In vivo Grade Recombinant Anti-human CD19 Monoclonal Antibody, Mouse IgG1 Kappa (Clone: SJ25C1)

References of Anti-human CD19 Monoclonal Antibody (Clone: SJ25C1)