Anti-human CD19 Monoclonal Antibodies for Flow Cytometry

PA007423.m2a: Non-conjugated Recombinant Anti-human CD19 Monoclonal Antibody (Clone: B43), Mouse IgG2a Kappa

Recombinant Mouse IgG2a Monoclonal Antibody. 
Clone: B43.
Isotype:  Mouse IgG2a kappa.
Source: The recombinant anti-human CD19 monoclonal antibody (clone: B43) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant B43 antibody specifically binds to the human CD19 protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007423.h1Fs: Non-conjugated Recombinant Anti-human CD19 Monoclonal Antibody (Clone: B43), Human IgG1 Fc Silent Kappa

Recombinant human IgG1 monoclonal antibody with Fc silent mutation.
Clone: B43.
Isotype: Human IgG1 kappa.
Source: The recombinant anti-human CD19 monoclonal antibody (clone: B43) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant B43 antibody specifically binds to the human CD19 protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The B43 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.

Syd Labs provides the following recombinant anti-human CD19 monoclonal antibodies for flow cytometry:

Non-conjugated Recombinant Anti-human CD19 Monoclonal Antibody (Clone: B43), Mouse IgG2a Kappa
Non-conjugated Recombinant Anti-human CD19 Monoclonal Antibody (Clone: B43), Human IgG1 Fc Silent Kappa

Background:

The B43 antibody binds to the human CD19 protein, a transmembrane protein expressed in all B lineage cells including human plasma cells. CD19 plays two major roles in human B cells: it acts as an adaptor protein to recruit cytoplasmic signaling proteins to the membrane, and works within the CD19/CD21 complex to decrease the threshold for B cell receptor signaling pathways. CD19 is a biomarker for B lymphocyte development, lymphoma diagnosis and can be utilized as a target for leukemia immunotherapies.

References of anti-human CD19 antibody (B43):

Fluorescent labeling in semi-solid medium for selection of mammalian cells secreting high-levels of recombinant proteins
Caron, A. W., et al. (2009). BMC Biotechnol. 2009 May;9(42):42. doi: 10.1186/1472-6750-9-42. PMID: 19432976
The light chain of the B43 antibody (anti-human CD19) was replaced in the pMPGB43k expression vector, which carries an enhanced CMV enhancer/promoter and a hygromycin resistance gene, to construct a new vector for recombinant protein expression. The B43 antibody (anti-human CD19 mAb) coding sequence was modified via PCR and cloned into the PmeI site of pMPGB34k, with a BamHI site added for further modifications. The study also isolated the IGF-1 coding sequence from murine cDNA for constructing pMPG-IGF-E5.
Tags: activity of B43 antibody; function of B43 antibody

Downregulation of major histocompatibility complex class I molecules by Kaposi's sarcoma-associated herpesvirus K3 and K5 proteins
Ishido, S., et al. (2000). J Virol. 2000 Jun;74(11):5300-9. doi: 10.1128/jvi.74.11.5300-5309.2000. PMID: 10799607
The B43 antibody (anti-human CD19) was used alongside W6/32 (MHC class I), RPA-T8 (CD8), M-A712 (transferrin receptor), and TU39 (MHC class II) for fluorescence-activated cell sorting and confocal immunofluorescence analyses. K3 and K5 proteins specifically downregulated MHC class I without affecting MHC class II, immunoglobulin M, or CD19 surface levels, as confirmed by these antibodies.
Tags: B43 antibody; B43 antibody for animal model

Large scale manufacturing of B43(anti-CD19)-genistein for clinical trials in leukemia and lymphoma
Myers, D. E., et al. (1998). Leuk Lymphoma. 1998 Apr;29(3-4):329-38. doi: 10.3109/10428199809068569. PMID: 9684930
The B43(anti-human CD19 antibody)-genistein immunoconjugate was manufactured at large scale for clinical trials in leukemia and lymphoma. The B43 antibody was purified from ascites fluid using protein A affinity chromatography, and its conjugation to genistein was optimized for high yield. Flow cytometry confirmed effective binding of B43-genistein to CD19+ leukemic cells, with cytotoxicity tested against leukemia cell lines.
Tags: B43 antibody for flow cytometry; B43 antibody for mouse tumor model

Pharmacokinetic features, immunogenicity, and toxicity of B43(anti-CD19)-pokeweed antiviral protein immunotoxin in cynomolgus monkeys
Kreitman, R. J., et al. (1997). Clin Cancer Res. 1997 Mar;3(3):325-37. doi: 10.1158/1078-0432.CCR-97-0137. PMID: 9815689
The B43(anti-human CD19)-pokeweed antiviral protein (PAP) immunotoxin was evaluated in cynomolgus monkeys for pharmacokinetics, immunogenicity, and toxicity. B43-PAP, constructed by linking the anti-CD19 monoclonal antibody B43 to PAP, killed >99.9% of primary leukemic progenitor cells from B-lineage ALL patients. The study revealed dose-limiting renal and cardiac toxicity in mice and severe renal tubular necrosis in monkeys.
Tags: B43 mAb for flow cytometry; B43 mAb for mouse tumor model

Membrane-associated CD19-LYN complex is an endogenous p53-independent and Bc1-2-independent regulator of apoptosis in human B-lineage lymphoma cells
Myers, D. E., et al. (1995). Proc Natl Acad Sci U S A. 1995 Oct;92(21):9575-9. doi: 10.1073/pnas.92.21.9575. PMID: 7568175
The B43 monoclonal antibody (anti-human CD19 mAb) was used to cross-link membrane-associated CD19, activating the LYN kinase signaling pathway and inducing apoptosis in B-lineage lymphoma cells in a p53- and Bcl-2-independent manner. The CD19-LYN complex was immunoprecipitated using B43 antibody, and flow cytometry confirmed high-level CD19 expression on lymphoma cells.
Tags: B43 mAb for cancer research; B43 monoclonal antibody

For more references about anti-human CD19 antibody (B43), please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following recombinant anti-mouse CD19 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD19 monoclonal antibody (Clone: 1D3) for flow cytometry

Syd Labs provides the following recombinant anti-human CD19 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD19 monoclonal antibody (Clone: SJ25C1) for flow cytometry
Recombinant Anti-human CD19 monoclonal antibody (Clone: FMC63) for flow cytometry
Recombinant Anti-human CD19 monoclonal antibody (Clone: B43) for flow cytometry

Syd Labs provides the following in vivo grade recombinant anti-mouse CD19 monoclonal antibodies:
Recombinant Anti-mouse CD19 monoclonal antibody (Clone: 1D3)

Syd Labs provides the following in vivo grade recombinant anti-human CD19 monoclonal antibodies:
Recombinant Anti-human CD19 monoclonal antibody (Clone: SJ25C1)
Recombinant Anti-human CD19 monoclonal antibody (Clone: B43)
Recombinant Anti-human CD19 monoclonal antibody (Clone: FMC63)