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SARS-CoV-2 Spike RBD Monoclonal Antibody

COVID-19 Virus Spike Antibody, 2019-nCoV S1 Antibody, SARS-CoV-2 RBD Antibody

Catalog No. Product Name Size List Price (US$) Quantity
PA007140 SARS-CoV-2 Spike RBD Monoclonal Antibody 200 ug 360.00
Description

PA007140: SARS-CoV-2 Spike RBD Monoclonal Antibody (Clone # CR3022)

Human IgG Monoclonal Antibody.
The monoclonal SARS-CoV-2 (2019-nCoV) spike antibody (S protein antibody, spike S1 antibody) was produced in mammalian cells.
Specificity/Sensitivity: The monoclonal SARS-CoV-2 (2019-nCoV) spike RBD antibody binds to SARS-CoV-2 and SARS-CoV spike RBD, S protein, and S1 subunit.
Applications: ELISA 1:10000, Flow Cytometry 1:500. It might be used as the human IgG antibody positive control for serological assays to detect SARS-CoV and SARS-CoV-2 infection.
Form of Antibody: 1 mg/ml in PBS, pH 7.4.
Clone: CR3022.
Isotype: hIgG1, H+L.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The monoclonal SARS-CoV-2 spike RBD antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

Please ask for a quote if you need the recombinant spike RBD monoclonal antibody as the human IgM antibody positive control for serological assays. To check more Syd Labs SARS-CoV-2 (2019-nCoV) Antigen and Antibody Reagents and Discovery Services

Background

The receptor binding domain (RBD) in the S1 subunit of the SARS-CoV-2 (2019-nCoV) spike (S) glycoprotein plays the most important roles in viral attachment, fusion and entry. The SARS-CoV and SARS-CoV-2 S proteins mediate viral entry into host cells by binding to a host receptor, angiotensin-converting enzyme 2 (ACE2), through the receptor-binding domain (RBD) in the S1 subunit, and then fusing the viral and host membranes through the S2 subunit. The ACE2-binding affinity of the Spike RBD of SARS-CoV-2 is 10- to 20-fold higher than that of SARS-CoV.

The SARS-CoV-2 S protein, especially its S1 subunit and RBD, has become a popular target of antibodies for diagnostics test, and proteins and antibodies for therapeutic drugs. The amino-acid sequence identity is around 74% between the RBDs in the SARS-CoV and SARS-CoV-2 S proteins, similar to the 77% identity between the whole S proteins of SARS-CoV and SARS-CoV-2. Such a high degree of sequence similarity raises the possibility that cross-reactive epitopes may exist. Although the SARS-CoV RBD polyclonal antibodies can recognize both viruses, only one out of a few published RBD monoclonal antibodies neutralizing SARS-CoV binds to SARS-CoV-2 spike RBD. Out of the 28 residues in the epitope of the S1 RBD monoclonal antibody, only 4 residues (14%) in SARS-CoV-2 S1 RBD are different from those in SARS-CoV S1 RBD. However, the S1 RBD mAb binds to SARS-CoV spike RBD (Kd = 1 nM) with a much higher affinity than to SARS-CoV-2 spike RBD (Kd = 115 nM, or 6.3 nM measured by another lab). It remains unreported that the S1 RBD neutralizing monoclonal antibody from a recovered SARS patient also behaves as a neutralizing antibody of SARS-CoV-2.

Syd Labs also provide custom antibody discovery services to screen more and better antibodies for therapeutic drugs, diagnostic immunoassays, and research reagents:

SARS-CoV-2-specific human neutralizing monoclonal antibody development service: If a client or collaborator provides PBMC from recovered COVID-19 patients or people with SARS-CoV-2 vaccines, Syd Labs can do single B cell sorting and culturing. We can send out about 70 ul supernatant per well (clonal single B cell) for quick screening. Then you tell us which single B cell clone you like. We sequence the antibody VH/VL and produce the recombinant human monoclonal antibody for your confirmation. The efficiency is much higher than antibody cloning and sequencing immediately after single B cell sorting; of course, the total cost is much lower. A few groups have used the single B cell method without culturing after sorting to identify some neutralizing monoclonal antibodies against the SARS-CoV-2 spike protein, especially its S1 RBD. It remains unknown how good they are to become therapeutic drugs. Using the strategy without culturing, one may screen antibodies from hundreds of single B cells after a lot of efforts; in contrast, using the strategy with culturing, one may easily screen antibodies from thousands of single B cells.

SARS-CoV-2 Spike S1 rabbit monoclonal antibody development service: We immunized rabbits with SARS-CoV-2 S protein S1 subunit, then did single B cell sorting and culturing and identified a number of anti-S1 or RBD-specific rabbit monoclonal antibodies. We measured affinity and pairing possibility of some recombinant rabbit mAbs, which can be used for clinical diagnostics to detect the SARS-CoV-2 antigen. Some rabbit mAbs even have the neutralization function to block the RBD-ACE2 interaction, which is critical for antibody discovery for therapeutics.

SARS-CoV-2 Nucleocapsid rabbit monoclonal antibody development service: Syd Labs immunized rabbits with SARS-CoV-2 Nucleocapsid protein (N protein), then did single B cell sorting and culturing. and identified a number of anti-Nucleocapsid rabbit monoclonal antibodies. High sensitivity: the affinity to the SARS-CoV-2 Nucleocapsid protein is as low as 5 pM. Antibody pairing: the recombinant rabbit mAbs bind to various epitopes of the SARS-CoV-2 Nucleocapsid protein.

SARS-CoV-2 spike S1 and Nucleocapsid VHH nanobody development service: Syd Labs immunized alpaca with SARS-CoV-2 S protein S1 subunit and Nucleocapsid protein, then used two strategies to screen alpaca nanobodies: single B cell sorting and culturing, and VHH phage display. The spike nanobodies may be good candidates for therapeutic drugs alone or by combination with other proteins or antibodies; the nucleocapsid nanobodies may be used for diagnostic test kits.

To know more about our human, rabbit, and alpaca (camelid) monoclonal antibody development services, please visit:

Human monoclonal antibody production services
Rabbit monoclonal antibody development services
Single B cell antibody cloning and sequencing services
Camelid monoclonal antibody production services

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