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Conditions of optimal recombinant SARS-CoV-2 S protein RBD-Fc-Avi performance should be determined experimentally by the investigator. Recombinant SARS-CoV-2 S protein RBD-His, biotinylated His-tag and Fc-fused S protein RBD, S protein RBD antibody and immunoassay kits, custom antibody and kit development services are available.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Source: Recombinant SARS-CoV-2 Spike RBD (S protein RBD, Arg319-Asn532) was expressed in mammalian cells with a Fc tag and Avi at the C-terminus.
Accession: QHD43416.1
Predicted molecular mass: 51.7 kDa. Due to glycosylation, the recombinant SARS-CoV-2 S protein RBD migrates to 60-62 kDa based on the Bis-Tris PAGE result.
Endotoxin: Less than 1 EU per ug by the LAL method.
Formulation: The recombinant SARS-CoV-2 Spike RBD-Fc-Avi protein was lyophilized from 0.22 um filtered solution in 20 mM PB (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Purity: > 95% by PAGE under reduced condition, and SEC-HPLC.
Shipping: The recombinant SARS-CoV-2 Spike RBD-Fc-Avi protein is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
Background
The S protein (spike) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on the S protein mediates this interaction. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
To check more Syd Labs SARS-CoV-2 (2019-nCoV) Antigen and Antibody Reagents and Discovery Services
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