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Recombinant SARS-CoV-2 Spike RBD

COVID-19 Virus Spike RBD, 2019-nCoV S1 RBD, Receptor-Binding Domain, S Protein RBD

Catalog No. Product Name Size List Price (US$) Quantity
BP003050 Recombinant SARS-CoV-2 Spike RBD-Fc-Avi 100 ug 350.00
BP003050 Recombinant SARS-CoV-2 Spike RBD-Fc-Avi 1 mg 2200.00
Description

BP003050: Recombinant SARS-CoV-2 Spike RBD-Fc-Avi (S protein RBD)

Source: Recombinant SARS-CoV-2 Spike RBD (S protein RBD, Arg319-Asn532) was expressed in mammalian cells with a Fc tag and Avi at the C-terminus.
Accession: QHD43416.1
Predicted molecular mass: 51.7 kDa. Due to glycosylation, the recombinant SARS-CoV-2 S protein RBD migrates to 60-62 kDa based on the Bis-Tris PAGE result.
Endotoxin: Less than 1 EU per ug by the LAL method.
Formulation: The recombinant SARS-CoV-2 Spike RBD-Fc-Avi protein was lyophilized from 0.22 um filtered solution in 20 mM PB (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Purity: > 95% by PAGE under reduced condition, and SEC-HPLC.

Shipping: The recombinant SARS-CoV-2 Spike RBD-Fc-Avi protein is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.

Background

The S protein (spike) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on the S protein mediates this interaction. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

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