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His-tagged recombinant human and cyno ACE2 protein (rhACE2-His and rcACE2-His), and the biotinylated ACE2 proteins are available. Please ask for a quote if you need ACE2 protein mutants or an ACE2 protein with different modification or components.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Source: recombinant human ACE2 protein (Gln18-Ser740) was expressed in mammalian cells with a Fc fusion and Avi at the C-terminus.
Accession: Q9BYF1
Predicted molecular mass: 111.2 kDa. Due to glycosylation, the rhACE2 protein migrates to 115-125 kDa based on the Bis-Tris PAGE result.
Endotoxin: Less than 0.5 EU per ug by the LAL method.
Activity: Immobilized biotinylated recombinant SARS-CoV-2 Spike S RBD with the His Tag at 1 ug/ml (100 ul/well). Dose response curve for rhACE2-Fc fusion protein with the EC50 of 81 ng/ml determined by ELISA. Conditions of optimal recombinant Human ACE2 protein (rhACE2-Fc) performance should be determined experimentally by the investigator.
Formulation: The recombinant human ACE2-Fc-Avi protein was lyophilized from 0.22 um filtered solution in 20 mM PB (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Purity: > 95% by PAGE under reduced condition, and SEC-HPLC.
Shipping: The recombinant human ACE2-Fc-Avi protein is shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
Questions and answers:
Question: Why did you put an Avi site at the C-terminus of your recombinant human ACE2-Fc-Avi protein (rhACE2-Fc)?
Answer: It is for site-specific biotinylation of the ACE2 protein, and the biotinylated ACE2 proteins are also available.
Question: Did you measure the biological activity of your recombinant human ACE2-Fc-Avi protein?
Answer: Yes. We immobilized biotinylated recombinant SARS-CoV-2 Spike S RBD with the His Tag at 1 ug/ml (100 ul/well), and measure dose response curve for rhACE2-Fc fusion protein with the EC50 of 81 ng/ml by ELISA. Conditions of optimal recombinant Human ACE2 protein performance should be determined experimentally by the investigator.
Question: Can your recombinant human ACE2-Fc-Avi protein be used to measure the function of an anti-S protein neutralizing antibody?
Answer: Yes, the rhACE2 protein binds to the RBD in the S protein S1 subunit.
Question: Do you have any antibody against the human ACE2 protein?
Answer: Yes, it is under development.
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Background
ACE2 (Angiotensin I Converting Enzyme 2) belongs to the angiotensin-converting enzyme family of dipeptidylcarboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. Diseases associated with ACE2 include Severe Acute Respiratory Syndrome (SARS) including Coronavirus Disease 2019 (COVID-19), and Neurogenic Hypertension. The SARS-CoV and SARS-CoV-2 (2019-nCoV) spike (S) proteins mediate viral entry into host cells by binding to the ACE2 receptor through the receptor-binding domain (RBD) of the S1 subunit, and then fusing the viral and host membranes through the S2 subunit. The ACE2-binding affinity of the Spike RBD of SARS-CoV-2 is 10- to 20-fold higher than that of SARS-CoV. Two trimeric SARS-CoV-2 spike proteins bind to an ACE2 dimer with the collectrin-like domain of ACE2 mediating homodimerization.
The binding of the S1 subunit to the extracellular peptidase domain of ACE2 on the surface of cells results in endocytosis and translocation of both the virus and the enzyme into endosomes located within cells. This entry process also requires priming of the S protein by the host serine protease TMPRSS2, which is a potential drug for inhibition. The recombinant human ACE2 (rhACE2) may also be a promising drug for those with intolerance to classic renin-angiotensin system inhibitors (RAS inhibitors) or in diseases where circulating angiotensin II is elevated. Infused rhACE2 has been evaluated in clinical trials for the treatment of SARS.
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