MB038-4G: 4G One-step RT-PCR Kit
MB038-4Gb: 4G One-step RT-PCR Kit with blue dye
The kit is provided in a convenient Master Mix format. The 2 x One-step Mix contains optimized buffer system, dNTP and loading dye; One-step Enzyme Mix contains optimized ratio of 4G Reverse Transcriptase, Murine RNase inhibitor, and hot start Taq DNA polymerase.
* High efficiency. Integrating the superior performance of 4G Reverse Transcriptase and hot-start Taq DNA Polymerase, with an optimized buffer system, the detection sensitivity of the 4G One Step RT-PCR Kit can reach 1 pg total RNA and can be amplified Fragments up to 10 kb.
* High purity. >98% homogeneous of the Taq DNA polymerase by SDS gel electrophoresis. No contamination detected in standard PCR test reactions.
* Reproducible results.
* Reverse transcription and PCR reactions are completed in one tube, no additional tube opening/pipetting operations are required, which improves detection throughput and reduces the risk of contamination.MB127-5G: 5G RT Supermix for qPCR with gDNA Elimination Function
* The 5G RT supermix for qPCR with gDNA elimination function is suitable for two-step RT-qPCR detection, and compatible with dye-based and probe-based qPCR, enabling high-performance gene expression analysis.
* Simple and fast operation: one-step genome clearance and reverse transcription.
* Higher reverse transcription efficiency and higher cDNA yields from all regions of RNA transcripts: based on the more efficient 5G Reverse Transcriptase.
* Flexible choice of primers: Different types of reverse transcription primers can be used flexibly for different experimental designs.
* Excellent cDNA stability: complete deactivation of thermal DNase and long-term storage of cDNA.
MB127-4G: 4G RT Supermix for qPCR with gDNA Elimination Function
* The 4G RT supermix for qPCR with gDNA elimination function is suitable for two-step RT-qPCR detection, and compatible with dye-based and probe-based qPCR, enabling high-performance gene expression analysis.
* Based on the highly efficient 4G Reverse Transcriptase, the super temperature tolerance ensures that the complex secondary structure of RNA can be opened under high temperature conditions to obtain longer cDNA.
* Quick and complete removal of genome contamination by the 4 × gDNA Removing Buffer: to ensure more reliable follow-up results, and simplify the design of qPCR primers, without the need to design primers across introns.
* Different primers are selected for different downstream applications.