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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant rat IgG2a isotype controls and recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Our Recombinant Anti-Mouse CD8a Monoclonal Antibody (Clone: 53-6.72) represents a sequence-defined, mammalian-expressed technological upgrade specifically engineered to deliver unmatched signal clarity in flow cytometry (FC/FACS) and immunohistochemistry (IHC-F). As a definitive cell-surface lineage marker, the mouse CD8a protein (T-cell surface glycoprotein CD8 alpha chain) serves as a critical orchestrator for identifying and gating cytotoxic T lymphocyte (CTL) populations within complex murine models of oncology, autoimmunity, and infectious diseases. By leveraging advanced recombinant engineering, our Anti-Mouse CD8a Clone 53-6.72 retains the exact high-fidelity variable regions of the classic 53-6.7 clone while completely overcoming the biological vulnerabilities of legacy hybridoma-derived reagents. Traditional hybridomas are notoriously prone to genetic drift, unexpected mutations, and contaminating light chains that degrade data quality; in contrast, this premium recombinant anti-mouse CD8a antibody guarantees absolute batch-to-batch consistency and a purified, ultra-pure signal profile, ensuring flawless long-term FACS gating stability.
To maximize analytical performance in multiplex panels and eliminate frustrating background noise during cell surface staining, our platform offers this premium clone in three application-targeted configurations. The classic Rat IgG2a Kappa format provides a trusted, non-conjugated purified framework that aligns perfectly with historical literature controls, making it ideal for customized secondary detection. For sophisticated profiling of myeloid-rich single-cell suspensions, our innovative Human IgG1 Fc Silent™ format features specific mutations engineered to completely abolish non-specific binding to endogenous cellular Fc receptors (FcγR), successfully eliminating tedious Fc-blocking steps and slashing background fluorescence. Furthermore, for ultra-sensitive multi-color panels requiring signal amplification, our site-directed Biotinylated Avi-Tag format introduces a precise biotin-conjugated modification at the C-terminus. Unlike random chemical labeling that frequently blocks the antibody-binding pocket and compromises affinity, our precision Avi-tag technology ensures 100% antigen-binding retention, providing an optimized platform for streptavidin-dye amplification, streptavidin agarose beads, or magnetic bead applications.
Recombinant Rat IgG2a Kappa Monoclonal Antibody.
Clone: 53-6.72.
Isotype: Rat IgG2a Kappa.
Source: The anti-mouse CD8a monoclonal antibody (clone: 53-6.72) was produced in mammalian cells.
Conjugation: non-conjugated.
Specificity/Sensitivity: The recombinant 53-6.72 antibody specifically binds to mouse CD8a.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: 53-6.72.
Isotype: Human IgG1 Fc Silent Kappa.
Source: The anti-mouse CD8a monoclonal antibody (clone: 53-6.72) was produced in mammalian cells.
Conjugation: non-conjugated.
Specificity/Sensitivity: The recombinant 53-6.72 antibody specifically binds to mouse CD8a.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
Clone: 53-6.72.
Isotype: Human IgG1 Fc Silent Kappa.
Source: The site-directed biotinylated anti-mouse CD8a monoclonal antibody (clone: 53-6.72) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant 53-6.72 antibody specifically binds to mouse CD8a.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The recombinant anti-mouse CD8a monoclonal antibody of clone 53-6.72 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Yes. Our recombinant anti-mouse CD8a flow cytometry antibody is strictly optimized and validated for high-resolution single-cell surface immunophenotyping via flow cytometry (FC/FACS). Additionally, it is fully verified for Immunohistochemistry on Frozen sections (IHC-F), allowing researchers to successfully map, gate, and track cytotoxic CD8+ T cell architectures within diverse murine tissue frameworks.
When profiling myeloid-dense samples (such as spleen or tumor-infiltrating lymphocytes), standard antibodies frequently bind non-specifically to cellular Fc receptors (FcγR), creating false positives. Our Human IgG1 Fc Silent™ Kappa format features targeted engineered mutations that completely abolish FcγR binding interaction. This slashes background fluorescence noise and saves valuable hands-on lab time by eliminating the requirement for complex, tedious Fc-blocking steps during cell surface staining.
Traditional chemical biotinylation randomly attaches biotin molecules to lysine residues, which frequently compromises or blocks the antibody's antigen-binding pocket. Our premium biotin-conjugated variant utilizes precision Avi-tag technology to enforce site-directed biotinylation exclusively at the C-terminus of the heavy chain. This elegant engineering guarantees 100% antigen-binding affinity retention, delivering supreme sensitivity and reproducibility when paired with streptavidin-fluorophore dyes or magnetic beads.
Legacy hybridoma cell lines are highly vulnerable to genetic drift, unexpected line mutations, or contamination from endogenous, non-specific myeloma light chains over time. Syd Labs' mammalian-expressed recombinant platform is entirely sequence-defined. Purified to >95% via stringent SDS-PAGE and HPLC assessments, this recombinant mouse CD8a antibody delivers an exceptionally clean signal profile with flawless batch-to-batch consistency, ensuring reproducible FACS gating across multi-year screening cohorts.
Yes, absolutely. Both our non-conjugated Rat IgG2a Kappa and Human IgG1 Fc Silent formats are formulated as pristine, preservative-free solutions, making them perfectly suited for customized primary staining panels. They can be effortlessly targeted by host-specific fluorescent secondary antibodies, granting researchers maximum flexibility when building complex, multi-color spectral flow cytometry matrices.
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