Background of Recombinant Anti-Digoxin Monoclonal Antibody (Clone: 26-10)
Our sequence-defined, mammalian-expressed recombinant anti-digoxin monoclonal antibody (Clone 26-10) is engineered to specifically recognize and bind to the cardiac glycoside digoxin. Unlike traditional antibody production methods, this recombinant framework guarantees exceptional batch-to-batch consistency and low endotoxin profiles. Recognized as an industry-standard clone, 26-10 binds digoxin with exceptionally high affinity, making it an indispensable capture and detection reagent for lateral flow immunoassays (LFIA), highly sensitive ELISAs, and therapeutic drug monitoring (TDM).
The digoxin/anti-digoxin detection system is widely utilized in advanced immunoassay signal amplification techniques. Because digoxin is entirely absent in natural mammalian biological samples, utilizing this highly specific anti-digoxin antibody clone 26-10 provides a pristine background with virtually zero cross-reactivity. This ensures a superior signal-to-noise ratio when detecting digoxin-conjugated targets attached to proteins, peptides, nucleic acids, or solid matrices.
To support diagnostic assay developers and industrial scale-up, Syd Labs offers comprehensive custom engineering for our anti-digoxin monoclonal antibody:
- Isotype & Fc Mutants: Custom formats including effector-silent mutants (such as Human IgG1 LALAPG) to completely eliminate non-specific binding in complex matrices.
- Affinity Tags: Recombinant insertion of Avi-tag, His-tag, or DYKDDDDK-tag for streamlined solid-phase immobilization.
- Direct Conjugates: Premium biotinylated anti-digoxin antibody variants, as well as direct labeling with an array of fluorophores, dyes, and enzymes (HRP/AP).
Please contact our scientific support team to request a quote for bulk orders, custom engineered mutants, or conjugate options.
PA007705.m1: Recombinant Anti-Digoxin Monoclonal Antibody (Clone: 26-10), Mouse IgG1 Kappa
Recombinant mouse IgG1 Monoclonal Antibody.
Clone: 26-10.
Isotype: Mouse IgG1 kappa.
Source: The recombinant anti-Digoxin monoclonal antibody (clone: 26-10) was produced in mammalian cells.
Specificity/Sensitivity: The recombinant anti-Digoxin monoclonal antibody (clone: 26-10) specifically binds to the cardiac glycosides digoxin, digitoxin, and digoxigenin with high-affinity.
Applications: Western Blot (WB), ELISA, Immunohistochemistry (IHC), Dot Blot and Immunocytochemistry.
Form of Antibody: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The recombinant anti-Digoxin monoclonal antibody (clone 26-10) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
References of Recombinant Anti-Digoxin Monoclonal Antibody (Clone: 26-10)
Key Application: Utilization of anti-digoxin antibodies in a rapid, portable lateral flow immunoassay (LFIA) for viral RNA detection.
Lin Chen, et al.
Microbiol Spectr. 2023 Sep 8;11(5):e0214423.
doi: 10.1128/spectrum.02144-23.
PMID: 37681992
The test line was prepared with anti-digoxin antibodies, while biotin was adopted to generate the control line. Subsequently, the reaction solution continues to flow along the strips to the test line, where it binds specifically to the anti-digoxin antibody covering this line. The detection of SFTSV RNA could be realized by generation of H1-H2 hybrid duplexes labeled with biotin and digoxin, which subsequently added to the LFIA test strips containing streptavidin conjugated with Alexa Fluor 647 as well as anti-digoxin antibodies. As a result, we found that all six probe sets and the corresponding target RNA sequences exhibited significantly higher fluorescence value compared to those without target RNA, suggesting each set of probes could successfully recognize and amplify the RNA target. Crucially, this method enabled stable detection of 500 copies/mL of SFTSV within 30 min using clinical serum samples.
(Tags: Lateral Flow Immunoassay; Anti-Digoxin Antibody; RNA Detection; LFIA Signal Amplification)
Key Application: Immobilization of anti-digoxin monoclonal antibodies on test lines for gold nanoparticle-based lateral flow biosensors.
Tingting Jiang, et al.
Antimicrob Agents Chemother. 2021 Feb 17;65(3):e01063-20.
doi: 10.1128/AAC.01063-20.
PMID: 33361302
Driven by capillary force, the PCR product-SA-AuNP composites migrate along the strip and aggregate on the T line through conjugation between T line-immobilized anti-digoxin monoclonal antibody (Ab) and the digoxin-label on the 5? end of common primers, which make the T line a golden red band due to the bathochromic effect of AuNPs. Briefly, biotin-BSA and anti-Dig Ab were preimmobilized via an XYZ3010 dispensing platform in a control line (C line) and a defined test line (T line), respectively, on a porous NC membrane. The duplex PCR amplicons with 5? terminal labeled with biotin and digoxin are recognized by streptavidin (SA)-AuNPs on the conjugate pad and then captured by anti-digoxin antibody through immunoreactions on the test line to produce a golden red line for detection. Meanwhile, the common antisense primers were modified with a 5? digoxin.
(Tags: Lateral Flow Biosensor; Anti-Digoxin Monoclonal Antibody; Gold Nanoparticle AuNP; LFIA Capture Reagent)
Key Application: Ratiometric fluorescence biosensing utilizing anti-Dig antibodies to trigger conformational switches in DNA tweezers.
Jia-Yang He, et al.
Anal Chem. 2021 Aug 24;93(33):11634-11640.
doi: 10.1021/acs.analchem.1c02444.
PMID: 34378382
Exploring the ratiometric fluorescence biosensing of DNA-templated biemissive silver nanoclusters (AgNCs) is significant in bioanalysis, yet the design of a stimuli-responsive DNA device is a challenge. A tweezer-like DNA architecture is assembled to populate fluorescent green- and red-AgNCs (g-AgNCs and r-AgNCs), aiming to produce a ratio signal via specific recognition of anti-Dig with two haptens (DigH). As such, the ratiometric fluorescence of r-AgNCs versus g-AgNCs is generated in response to anti-Dig, achieving reliable quantization with a limit of detection at the picomolar level. Based on the fast stimulated switch of unique DNA tweezers, our ratiometric strategy of dual-emitting AgNCs would provide a new avenue for a variety of bioassays. When two DigHs in proximity recognize and bind anti-Dig, the conformation switch of these tweezers resultantly occurs, taking g-AgNCs away from G18 for "green-off" and bringing r-AgNCs close to G18 for "red-on".
(Tags: Fluorescence Biosensing; Anti-Digoxin Antibody; DNA Tweezers; Biosensor Signal Amplification)
Key Application: Development of a nanoparticle-based antigen delivery system for the production of anti-digoxin antibodies.
Nazli Kashani Javid, et al.
Pharm Nanotechnol. 2023 May 22.
doi: 10.2174/2211738511666230522142410.
PMID: 37218193
Nanoparticles have received more and more attention in the vaccine and drug delivery systems field due to their specific properties. In particular, alginate and chitosan have been known as the most promising nano-carries. The present study aimed to develop alginate/chitosan nanoparticles as a carrier of Digoxin-KLH to promote the immune response by improving the hyper-immunization of animals. The synthesized nanoparticles of 52 nm in diameter, 0.19 in PDI, and -33mv in zeta potential were considerably unparalleled and characterized by SEM, FTIR, and DSC. The invitro conjugate release profile, release kinetics, and mechanism of conjugate release from the nanoparticles were studied under simulated physiological conditions for various incubation periods.
(Tags: Anti-Digoxin Monoclonal Antibody Production; Digoxin-KLH Conjugate; Antigen Delivery System)
For more references about recombinant anti-digoxin antibody please contact our scientific support team with
message@sydlabs.com.
Syd Labs provides the following recombinant anti-Biotin/Digoxin/DNP/Fluorescein monoclonal antibodies:
Recombinant Anti-Biotin Monoclonal Antibodies (Clone F1)
Recombinant Anti-Biotin Monoclonal Antibodies (Clone M33)
Recombinant Anti-Digoxin Monoclonal Antibodies (Clone 5A7)
Recombinant Anti-Digoxin Monoclonal Antibodies (Clone 26-10)
Recombinant Anti-DNP Monoclonal Antibodies (Clone DNP.m3)
Recombinant Anti-Fluorescein Monoclonal Antibodies (Clone 4-4-20)
Recombinant Anti-Fluorescein Monoclonal Antibodies (Clone 4M5.3)